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| - | <h2 style='margin-top:0cm;margin-right:0cm;margin-bottom:7.2pt;margin-left:
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| - | 0cm; ;border:none;padding:0cm'><span class=mw-headline><span
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| - | style='font-size:16.0pt;font-family:"Calibri","sans-serif";font-weight:normal'>Before
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| - | we do</span></span></h2>
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| - | </div>
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| - |
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| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt; '><span style='font-family:"Calibri","sans-serif"'>Personel
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| - | safety is one of the top priorities in the lab. Before starting lab work, we
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| - | attended a lab safety training program provided by our teacher Mr.Bestepe. We
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| - | were familiarized with the operation of lab equipment and nature of chemicals
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| - | we would be using in the lab.</span></p>
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| - |
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| - | <p style='margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:15.75pt;
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| - | '><span style='font-family:"Calibri","sans-serif";0'>We used our team funds to buy disinfectant white medical uniforms.
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| - | Before we enter the lab, everyone must put on the uniform and go through the disinfectant
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| - | door to make sure that they are clean. Some chemicals may be harmful to human
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| - | skin, so everyone is required to wear safety gloves, to isolate the reagents
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| - | from our skin. When important and strict steps are required, we have to spray
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| - | the alcohol on our gloves to be sure that our gloves are cleaned. These are
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| - | universal precautionary measures.</span></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
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| - |
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| - | <p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
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| - | style='font-size:16.0pt; '>1. Would any of your project ideas
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| - | raise safety issues in terms of researcher safety, public safety, environmental
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| - | or ethical safety?</span></p>
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| - |
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| - | </div>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>a.<span
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| - | class=apple-converted-space> </span><b>Reseacher safety</b>: <br>
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| - | Everyone on the team obtained laboratory safety training through the Machinery
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| - | of Life/Foundations of Molecular Medicine and Bioengineering CAPS Class and had
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| - | experience with bacterial culture (in E.coli) and genetic transformation
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| - | (inserting pGLO into E.coli) prior to beginning the project.</span></p>
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| - |
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| - | <p class=MsoNormal><b><span style='font-size:12.0pt;line-height:115%'>During
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| - | the work, our team took the following safety precautions:</span></b></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Lab coats,
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| - | gloves and goggles were worn at all times.</span></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>All
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| - | equipment (both disposable and autoclaved) was sterile.</span></p>
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| - |
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| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:38.4pt;text-indent:
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| - | -18.0pt;line-height:14.65pt'><span style='font-size:12.0pt'>1.<span
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| - | style='font:7.0pt "Times New Roman"'> </span></span><span
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| - | style='font-size:12.0pt'>The bench and other work surfaces were sterilized with
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| - | water and bleach before and after lab work.</span></p>
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| - |
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| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:38.4pt;text-indent:
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| - | -18.0pt;line-height:14.65pt'><span style='font-size:12.0pt'>2.<span
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| - | style='font:7.0pt "Times New Roman"'> </span></span><span
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| - | style='font-size:12.0pt'>Because cyanobacteria can pose a risk if ingested,
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| - | there was no food or drink in the lab.</span></p>
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| - |
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| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;line-height:
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| - | 14.3pt'><span style='font-size:12.0pt'>There were no lit burners or other fire
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| - | during lab work.</span></p>
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| - |
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| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>b.<span
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| - | class=apple-converted-space> </span><b>Public Safety</b>: When released by
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| - | accident, our parts and materials actually cause no damage to the general
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| - | public. Due to the unability of E.coli strains, TOP10 and BL21 to survive out
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| - | of the lab, they cannot pose any risk to the safety and health of the general
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| - | public.</span></p>
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| - |
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| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>c.<span
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| - | class=apple-converted-space> </span><b>Environment</b>: E. coli strains TOP10
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| - | and BL21 have very limited ability to survive outside the laboratory; so that,
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| - | it would be unable to survive or disseminate. Therefore, there is no specific
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| - | environmental risk associated with the E. coli strains. All bacterial wastes
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| - | are kept in 10% bleaching solution for one day, and then, are autoclaved to be
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| - | sterilized. Yet, undesired GMOs may achieve ecologically harmful features.</span></p>
| |
| - |
| |
| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><b><span style='font-family:"Calibri","sans-serif"'>d.Ethical
| |
| - | Safety</span></b></p>
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| - |
| |
| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>We
| |
| - | recognize that our Lignin Peroksydase gene was isolated by Mr.Bestepe and that
| |
| - | we only get use it per his permission. Accordingly, we have kept him informed
| |
| - | on our work with it and the possibility of this gene becoming readily avaliable
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| - | in the Standard Registry of Biological Parts.</span></p>
| |
| - |
| |
| - | <div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
| |
| - | style='font-size:16.0pt; '>2. Do any of the new BioBrick parts
| |
| - | (or devices) that you made this year raise safety issues?</span></p>
| |
| - |
| |
| - | </div>
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| - |
| |
| - | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
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| - | 14.3pt'><span style='font-size:12.0pt; '>No, none of the
| |
| - | BioBricks that were made by us this year raise any safety issues. They are a
| |
| - | 100% safe for the environment, general public, and our team members handling
| |
| - | them. </span></p>
| |
| - |
| |
| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Lignin
| |
| - | Peroksydase</span><span class=apple-converted-space><span style='font-family:
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| - | "Calibri","sans-serif"'> </span></span><span style='font-family:"Calibri","sans-serif"'>is
| |
| - | a comletely safe and common enzyme. Being in many organisms and commonly used
| |
| - | in many applications, Lignin Peroksydase is still considered one of the safest
| |
| - | enzymes. The DegradEcolor is nothing but an mRNA sequence which works as a
| |
| - | simple DegradEcolors in organisms. The DegradEcolor won't even have the
| |
| - | slightest effect on any external being.</span></p>
| |
| - |
| |
| - | <div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
| |
| - | style='font-size:16.0pt; '>3. Is there a local biosafety group,
| |
| - | committee, or review board at your institution?</span></p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Our
| |
| - | institution has its own biosafety rules. Rules for laboratory use, general principles,
| |
| - | prevention from hazardous materials and application of emergency intervention
| |
| - | in case of accident are included. In Turgut Ozal University Medical School,
| |
| - | Laboratory and Patient-Employee Safety Committee is responsible for control as
| |
| - | well as biosafety of laboratories and safety of patients and employees. This
| |
| - | committee works under one of the vice medical director of Turgut Ozal
| |
| - | University Hospital, Prof. Dr. Mehmet Gunduz. Safety and security issues are
| |
| - | found sufficient enough that no change is considered as necessary.<span
| |
| - | class=apple-converted-space> </span><i>(Further questions can be directed
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| - | to Prof. Gunduz, tel: +90-312-203 5103, mgunduz@fatih.edu.tr)</i><span
| |
| - | class=apple-converted-space> </span>Our advisor provided us with biosafety
| |
| - | and lab training before starting our project. In the training, general safety
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| - | rules of laboratory use, prevention from hazardous chemicals and solutions as
| |
| - | well as emergency intervention in case of accident were included such as:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
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| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Eating, drinking, storing food and smoking are
| |
| - | absolutely not allowed.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
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| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Mouth pipetting is not allowed; modern pipettes are used
| |
| - | in lab.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
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| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Hazardous wastes and ordinary wastes are separated and
| |
| - | cautiously disposed.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
| |
| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Lab coats are obligatory to wear in the lab and during
| |
| - | on-going experiments.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
| |
| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>In electrophoresis room, lab coat, protective eyewear,
| |
| - | lab masks and gloves have to be worn. In the case of use of<span
| |
| - | class=apple-converted-space> </span><b>EtBr</b>; extra caution is
| |
| - | required.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
| |
| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Washing hands after any experiment and after touching
| |
| - | anything related with viable material is obliged.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
| |
| - | -18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
| |
| - | style='font:7.0pt "Times New Roman"'> </span></span><span
| |
| - | style='font-size:12.0pt'>Air conditioning is kept closed during on-going
| |
| - | experiments in order to avoid possible infections of spores and bacteria.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:1.2pt;line-height:14.3pt'><span
| |
| - | style='font-size:12.0pt'> </span></p>
| |
| - |
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| - | <div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
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| - |
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| - | <p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
| |
| - | style='font-size:16.0pt; '>4. Do you have any other ideas how to
| |
| - | deal with safety issues that could be useful for future iGEM competitions? How
| |
| - | could parts, devices and systems be made even safer through biosafety
| |
| - | engineering?</span></p>
| |
| - |
| |
| - | </div>
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| - |
| |
| - | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
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| - | normal'><span style='font-size:12.0pt; '><br>
| |
| - | To deal with safety issues, for future iGEM competition, all Biobrick part
| |
| - | should contian a potential knockout device. Also, Biobrick parts can be
| |
| - | screened for potentially dangerous pathogens through the use of a variety of
| |
| - | software that can be found at universities like Turgut Ozal UNİVERİTY Medical
| |
| - | School.</span></p>
| |
| - |
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| - | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
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| - | normal'><span style='font-size:12.0pt'> </span></p>
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| - |
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| - | <div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm;
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| - | '>
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| - |
| |
| - | <h2 style='margin-top:0cm;margin-right:0cm;margin-bottom:7.2pt;margin-left:
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| - | 0cm; ;border:none;padding:0cm'><span class=mw-headline><span
| |
| - | style='font-size:16.0pt;font-family:"Calibri","sans-serif";font-weight:normal'>Something
| |
| - | dangerous</span></span></h2>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt; '><span style='font-family:"Calibri","sans-serif"'>When
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| - | we approach the operating floor, especially doing the electrophoresis (to
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| - | separate the different part of the plasmid) and usually the gel we used was
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| - | made of carcinogen. Therefore, when students are doing this step, we mustn’t
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| - | touch the floor, even can’t get very close to it. Another harmful thing is the
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| - | ultraviolet rays. Because the rays which used by lab’s equipment have strong
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| - | radiation, so we can not obverse it directly, we have to use an idiosyncratic
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| - | board to see it, in order to reduce the harm for our eyes.</span></p>
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| - | <p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;line-height:
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| - | 14.3pt'><span style='font-size:12.0pt'> </span></p>
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| - |
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| - | <p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
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| - | 0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Turkey
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| - | has national biosafety regulations and the link is given as<i>(</i></span><span
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| - | style='font-family:"Calibri","sans-serif"'><a
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| - | href="http://www.tbbdm.gov.tr/en/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx"><i><span
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| - | style='color:windowtext'>http://www.tbbdm.gov.tr/en/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx</span></i></a></span><i><span
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| - | style='font-family:"Calibri","sans-serif"'>)</span></i><span style='font-family:
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| - | "Calibri","sans-serif"'>.</span></p>
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
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| - |
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| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
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| - |
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| - | </div>
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| - |
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| - | </body>
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| - | </head>
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| - | </html>
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