Team:Nanjing NFLS

From 2014hs.igem.org

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|align="center"|[[Team:Nanjing_NFLS | Team Nanjing_NFLS]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Nanjing_NFLS Official Team Profile]
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===Team===
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南京外国语学校坚持以“培育有中国灵魂、世界胸怀的现代人”为目标，倡导“求实、奋进、博学、谦逊”的校风和“严、新、细、活”的教风，致力于培养有外语特长、文理兼通、综合能力强的国际化、复合型人才。
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[http://www.nfls.com.cn/ 南京外国语学校]
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CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are often associated with cas genes which code for proteins that perform various functions related to CRISPRs. The CRISPR-Cas system is a novel technique of gene editing, including silencing, enhancing or changing specific genes. By inserting a plasmid containing cas genes and specifically designed CRISPRs, the organism's genome can be cut at any desired location.
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Based on the CRISPR-Cas system, the team plans to design a set of biological machine repair kit including tools to delete and/or replace biobricks in building complex synthetic biology devices and systems. With this repair kit, biological machines malfunction due to mutation and many other causes can be repaired by having one or a few certain biobricks replaced instead of re-building the entire machine.  
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The procedures include choosing the ideal promoter and terminator with universality for knock-in and designing the DNA sequences for a donor plasmid with homogenous arms and guide-RNA, which then are transfected into the host cells with Cas9. The proof of principle kits will be tested on basic PRCT protein generators with EGFP and RFP as reporters and applied to replace causative genes with non-causative alleles once with proven validity.
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===Notebook===
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