Team:Acton-BoxboroughRHS

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<div id="template" style="text-align: center; font-weight: bold; font-size: large; /*color: #f6f6f6*/; padding: 5px;">
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        <title>Welcome to the ABRHS iGEM team</title>
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have the following information on your wiki:
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:Acton-BoxboroughRHS/Our_Pets</span>
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                <p style="font-family:Arial;font-size:45px">ABRHS iGEM Site</p>
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                <img height="220px" width="950px" src="https://c9.io/ab_igem/igem2014/workspace/copy.jpg" alt="ABRHS">  
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            <p style="font-family:arial">Note that this is a prototype site. For the real wiki, go <a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS">here</a>.</p>
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        <div id="navbar">
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        <ul>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS" id="onlink">Home</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Project">Project</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Notebook">Notebook</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Team">The Team</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Simulations">Simulations</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Safety">Safety</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Research">Research</a></li>
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            <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Labs">Labs</a></li>
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        <br></br>
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        <h style="font-size:40px;font-family:Arial">Home</h>
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        <br></br>
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        <div id="cnt1">
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        <h><b>Goal</b></h>
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        <p>The goal of ABRHS was<br> to create a safe alternative<br>to theproduction of<br>digested Kopi Luwak coffee<br>beans. For the iGEM<br>competition, we genetically<br>engineered the bacteria,<br>E. coli, to produce digestive<br>enzymes that would do the<br>same work.<br><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Project">(read more)</a><br></p>
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            function render(){
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                ctx.font="40px Arial";
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                ctx.fillText("Project E. coli Luwak:",10,50);
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                ctx.fillStyle="rgb("+(-50+time)%255+","+(-50+time*3)%255+","+(-50*time*5)%255+")";
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                ctx.fillText("Protecting the Civet,",10,100);
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        <h><b>The Team</b></h>
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        <p>Our team consists of <br>eleven students from <br>the Acton-Boxborough<br>Regional High School.<br> Together, we promote<br>respect for self,<br>others, and learning.<br><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Team">Meet the Team</a><br></p>
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        </div>
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        <img height="400px" width="446px" style="border-radius:10px" src="http://upload.wikimedia.org/wikipedia/commons/thumb/c/ce/Asian_Palm_Civet_Over_A_Tree.jpg/669px-Asian_Palm_Civet_Over_A_Tree.jpg">
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        <p>
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        <a href="http://en.wikipedia.org/wiki/File:Asian_Palm_Civet_Over_A_Tree.jpg">Asian Palm Civet</a>, photographed by Praveenp under Wikimedia Commons
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        </p>
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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            <p id="main_content">The Kopi Luwak</p>
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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        <h style="font-size:40px;font-family:Arial;border:1px solid rgb(0,0,0);border-radius:10px">Acknowlegements</h>
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|align="center"|[[Team:Acton-BoxboroughRHS | Team Acton-BoxboroughRHS]]
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        <img src="data:image/jpeg;base64,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">
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+
        <p><a href="https://www.ll.mit.edu/">MIT Lincoln Labs</a></p>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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        <br>
-
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Acton-BoxboroughRHS Official Team Profile]
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        <br>
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|}
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        <div style="background-color:rgb(10,10,10);">
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        <img src="https://www.neb.com/~/media/NebUs/Navigation/logo-NEB.png">
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==Team==
+
        </div>
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        <p><a href="https://www.neb.com/products/c2987-neb-5-alpha-competent-e-coli-high-efficiency">New England BioLabs</a></p>
-
Our team of sixteen students is from the Acton-Boxborough Regional High School, includes Akash Kapoor, Chris Kuffner, Mayank Mali, Benjamin Pervier, Madhuri Jois, Wynne Yao, Charles Taussig, Tara Jawahar, Rachel Tao, Katherine Liu, Barry Huang, Ben Stern, Justin Hong, William Huang, Victoria Chen, and Anusha Purakayastha, and is coached by Aaron Mathieu.
+
        <br>
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        <br>
-
The team profile can be found [https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Acton-BoxboroughRHS here].
+
        <img src="http://api.ning.com/files/rf*hGVlTe0kDQp8m*YwHGE1xfUnJtFFlPgfB1DcAXbG1gypSxQNzunHtoTRsuFsN8hYq3NPTJ9fUNMLgaC03lH5-2*1eSOGZ/biobuildergreen.png">
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+
        <p><a href="http://biobuilder.org/">BioBuilder</a></p>
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==Project==
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        <br>
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Our team has finalized our large amalgamation of thoughts to a few possible projets:
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        <br>
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=====Fat/sugar/protein converter=====
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        <br></br>
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=====Genetically produced fuel/BioDeisel=====
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        <br></br>
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=====Kopi Luwak Coffee=====
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        <div style="background-color:#FF3333;font-family:Arial;font-size:10px;width:200px;border-radius:5px">
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:3-5 certain enzymes get rid of bitter proteins and carbohydrates, making coffee more sweet.
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        <p>End of page</p>
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:Electronic nodes can be used to detect changes in certain composition of beans.
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        </div>
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=====Bacteria that absorbs CO2/fossil fuel by product/catalytic converter=====
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=====Skin regenerating Bacteria=====
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=====Bacteria with memory/synthetic neurons=====
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        <!--
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=====Synthetic/modified mitochondria or chloroplast=====
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        <img src="Jellyfish.jpg">
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<br>
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        <img src="test.jpg">
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<br>
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        -->
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===Final Project Decision===
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-
:Kopi Luwak coffee is the rarest and most expensive type of coffee in the world. This notorious beverage
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-
is made from the feces of the Asian Palm Civet (Paradoxurus hermaphroditus) or Luwak, a cat-like omnivore that fills the niche of a racoon in Asia. The Asian Palm Civet eats raw coffee berries. As the berries are digested, enzymes in the animal’s digestive tract break down components of the coffee bean that are responsible for its bitter taste. However, the beans themselves are not digested. The civet only digests the fleshy outer layer, so when it defecates, it leaves clumps of coffee beans that have been processed by its enzymes. The beans are then cleaned, roasted, and brewed to make the Kopi Luwak coffee.
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:Due to the complexity of this process, Kopi Luwak is a very expensive item at $600 per pound.
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The outrageous price has made Kopi Luwak a novelty for the rich. Its allegedly excellent flavor is sadly something that most people are unable to afford. Another drawback of Kopi Luwak is that demand has driven businesses to animal cruelty in order to keep up production. A small civet farming industry has tens of thousands of civets living in battery cages being force fed coffee berries. Civets, being omnivores, are no more capable of surviving on coffee than humans. As a result their population is diminishing.
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        <!--this is a comment!!-->
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:We propose to make this process more humane, efficient, and sanitary by using bacteria instead of
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        <script type="text/javascript"></script>
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civets to process coffee berries into Kopi Luwak beans. We will accomplish this by inserting genes that code for proteins found in the civet’s digestive tract into a hardy bacteria that can withstand the pH levels required for the proteins to operate.  We intend to add genes for salivary amylase, pepsin, pancreatic amylase, trypsin, and chymotrypsin, sequenced from palm civets or closely-related species. Once the bacteria have been transformed, we will attempt to  simulate the digestive process of the civet on coffee berries and analyze our results.
+
    </body>
-
:Although this project seems unconventional, putting animal enzymes in bacteria has been done before
+
   
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to great effect.  Take rennet cheese for an example. In order to obtain the enzymes required to produce this cheese, a calf must be slaughtered; the material (rennet) is taken from the dead animal’s digestive tract. Now, due to growing demand for this cheese, bacteria containing the rennet enzymes are used instead. Today, the sale of these cheeses is a popular and profitable industry. By putting animal enzymes in bacteria, we create a digestive platform that is capable of processing more than just coffee berries.
+
</html>
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+
-
==Notebook==
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-
 
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-
===First Few Weeks===
+
-
In the first few weeks, we had introduced iGEM to students, taken sneek-peeks on projects done by past iGEM teams, and done small exercises. One example of a demonstration was modelling the process of producing a plasmid of recombinant DNA with restriction enzymes and ligase using pipe cleaners, candies, labels and scissors.
+
-
 
+
-
===Up to February 26 2014===
+
-
Our team has done some moderate research on final topics. The final project will be picked soon.
+
-
 
+
-
These are a list of the possible candidates:
+
-
<br>
+
-
:Fat/sugar/protein converter
+
-
:Genetically produced fuel/BioDeisel
+
-
:Kopi Luwak Coffee
+
-
:Bacteria that absorbs CO2/fossil fuel by product/catalytic converter
+
-
:Skin regenerating Bacteria
+
-
:Bacteria with memory/synthetic neurons
+
-
:Synthetic/modified mitochondria or chloroplast
+
-
<br>
+
-
<br>
+
-
===February 27 2014 Narrowing it down.===
+
-
:Genetically produced fuel/BioDeisel
+
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:Kopi Luwak Coffee (''COLI'' Luwak Coffee)
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:Bacteria with memory/synthetic neurons / Battery?
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==Results/Conclusions==
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What did you achieve over the course of your semester?
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==Safety==
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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==Attributions==
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Who worked on what?
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==Human Practices==
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What impact does/will your project have on the public? 
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==Fun!==
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What was your favorite team snack?? Have a picture of your team mascot?
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<forum_subtle />
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Latest revision as of 02:56, 14 September 2014

Welcome to the ABRHS iGEM team





Home

Goal

The goal of ABRHS was
to create a safe alternative
to theproduction of
digested Kopi Luwak coffee
beans. For the iGEM
competition, we genetically
engineered the bacteria,
E. coli, to produce digestive
enzymes that would do the
same work.
(read more)





The Team

Our team consists of
eleven students from
the Acton-Boxborough
Regional High School.
Together, we promote
respect for self,
others, and learning.
Meet the Team

Asian Palm Civet, photographed by Praveenp under Wikimedia Commons

The Kopi Luwak





Acknowlegements


MIT Lincoln Labs



New England BioLabs



BioBuilder







End of page

Retrieved from "http://2014hs.igem.org/Team:Acton-BoxboroughRHS"