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        Engineering<br />
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    <span style="font-family: 'Lato'; font-size: 18px;">E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes</span>
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            <h2>Results</h2>
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            <p>Construction of the proposed biosensors was not completed
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              successfully due to what appear to be flawed ligations and/or low
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              efficiency competent cells. The necessary methodology and
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              procedures have been laid down for further work on construction,
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              development and improvement of the biosensor system in the future.
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              Further work include the completion of the biosensors, proof of
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              concept growing bacteria in absence and presence of stimuli and
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              application of biosensors to a range of samples from specific
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              points distributed all along Xochimilco lake. Results of these
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              tests could be then applied in the construction a map of the lake
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              specifying the most critical points that need to be treated.</p>
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            <p class="8u center">Figure 1. The agarose gel shows the unsuccesful
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              ligations, represented on the right.</p>
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          </section>
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    <div id="third-text"> Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to th ese proteins will have an immense commercial, industrial, agricultural, and health impact.</div> 
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Latest revision as of 03:26, 21 June 2014

iGEM San Diego

Engineering
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to th ese proteins will have an immense commercial, industrial, agricultural, and health impact.

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