Data notebook.html

Revision as of 03:03, 21 June 2014 (view source)

(Created page with "<html> <head> <style> *{ margin:0; padding:0; } body{ background:#ccc url(../images/DNA.jpg) repeat top left; font-family: Arial, Helvetica, sans-serif; color:#444; font-si...")

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Latest revision as of 03:20, 21 June 2014 (view source)

Gonzila (Talk | contribs)

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~~<html>~~

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~~<head>~~

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~~<style>~~

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*{

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~~margin:0;~~

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~~padding:0;~~

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}

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~~body{~~

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~~background:#ccc url(../images/DNA.jpg) repeat top left;~~

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~~font-family: Arial, Helvetica, sans-serif;~~

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~~color:#444;~~

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~~font-size:12px;~~

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~~color: #000;~~

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}

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~~h1{~~

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~~color:#000000;~~

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~~font-size:40px;~~

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~~margin:20px 0px 0px 20px;~~

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}

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~~span.reference{~~

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~~font-family:Arial;~~

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~~display:block;~~

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~~font-size:12px;~~

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~~text-align:center;~~

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~~margin-bottom:10px;~~

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}

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~~span.reference a{~~

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~~color:#000;~~

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~~text-transform:uppercase;~~

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~~text-decoration:none;~~

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~~margin:0px 20px;~~

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}

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~~span.reference a:hover{~~

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~~color:#ddd;~~

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}

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~~/* Booklet jQuery Plugin Style*/~~

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~~.booklet{~~

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~~-moz-box-shadow:0px 0px 1px #fff;~~

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~~-webkit-box-shadow:0px 0px 1px #fff;~~

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~~box-shadow:0px 0px 1px #fff;~~

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~~-moz-border-radius:10px;~~

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~~-webkit-border-radius:10px;~~

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~~border-radius:10px;~~

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}

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~~.booklet .b-wrap-left {~~

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~~background:#fff url(../images/left_bg.jpg) no-repeat top left;~~

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~~-webkit-border-top-left-radius: 10px;~~

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~~-webkit-border-bottom-left-radius: 10px;~~

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~~-moz-border-radius-topleft:10px;~~

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~~-moz-border-radius-bottomleft: 10px;~~

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~~border-top-left-radius: 10px;~~

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~~border-bottom-left-radius: 10px;~~

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}

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~~.booklet .b-wrap-right {~~

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~~background:#efefef url(../images/right_bg.jpg) no-repeat top left;~~

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~~-webkit-border-top-right-radius: 10px;~~

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~~-webkit-border-bottom-right-radius: 10px;~~

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~~-moz-border-radius-topright: 10px;~~

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~~-moz-border-radius-bottomright: 10px;~~

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~~border-top-right-radius: 10px;~~

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~~border-bottom-right-radius: 10px;~~

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}

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~~.booklet .b-counter {~~

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~~bottom:10px;~~

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~~position:absolute;~~

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~~display:block;~~

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~~width:90%;~~

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~~height:20px;~~

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~~border-top:1px solid #ddd;~~

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~~color:#222;~~

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~~text-align:center;~~

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~~font-size:12px;~~

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~~padding:5px 0 0;~~

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~~background:transparent;~~

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~~-moz-box-shadow:0px -1px 1px #fff;~~

-

~~-webkit-box-shadow:0px -1px 1px #fff;~~

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~~box-shadow:0px -1px 1px #fff;~~

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~~opacity:0.8;~~

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}

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~~.book_wrapper{~~

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~~margin:0 auto;~~

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~~padding-top:50px;~~

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~~width:905px;~~

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~~height:540px;~~

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~~position:relative;~~

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~~background:transparent url(../images/bg.png) no-repeat 9px 27px;~~

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}

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~~.book_wrapper h1{~~

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~~color:#13386a;~~

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~~margin:5px 5px 5px 15px;~~

-

~~font-size:26px;~~

-

~~background:transparent url(../images/h1.png) no-repeat bottom left;~~

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~~padding-bottom:7px;~~

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}

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~~.book_wrapper p{~~

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~~font-size:16px;~~

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~~margin:5px 5px 5px 15px;~~

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}

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~~.book_wrapper a.article,~~

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~~.book_wrapper a.demo{~~

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~~background:transparent url(../images/circle.png) no-repeat 50% 0px;~~

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~~display:block;~~

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~~width:95px;~~

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~~height:41px;~~

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~~text-decoration:none;~~

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~~outline:none;~~

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~~font-size:16px;~~

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~~color:#555;~~

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~~float:left;~~

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~~line-height:41px;~~

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~~padding-left:47px;~~

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}

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~~.book_wrapper a.demo{~~

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~~margin-left:50px;~~

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}

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~~.book_wrapper a.article:hover,~~

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~~.book_wrapper a.demo:hover{~~

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~~background-position:50% -41px;~~

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~~color:#13386a;~~

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}

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~~.book_wrapper img{~~

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~~margin:10px 0px 5px 35px;~~

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~~width:300px;~~

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~~padding:4px;~~

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~~border:1px solid #ddd;~~

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~~-moz-box-shadow:1px 1px 1px #fff;~~

-

~~-webkit-box-shadow:1px 1px 1px #fff;~~

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~~box-shadow:1px 1px 1px #fff;~~

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}

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~~.booklet .b-wrap-right img{~~

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~~border:1px solid #E6E3C2;~~

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}

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~~a#next_page_button,~~

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~~a#prev_page_button{~~

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~~display:none;~~

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~~position:absolute;~~

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~~width:41px;~~

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~~height:40px;~~

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~~cursor:pointer;~~

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~~margin-top:-20px;~~

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~~top:50%;~~

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~~background:transparent url(../images/buttons.png) no-repeat 0px -40px;~~

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}

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~~a#prev_page_button{~~

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~~left:-30px;~~

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}

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~~a#next_page_button{~~

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~~right:-30px;~~

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~~background-position:-41px -40px;~~

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}

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~~a#next_page_button:hover{~~

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~~background-position:-41px 0px;~~

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}

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~~a#prev_page_button:hover{~~

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~~background-position:0px 0px;~~

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}

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~~.loading{~~

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~~width:160px;~~

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~~height:56px;~~

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~~position: absolute;~~

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~~top:50%;~~

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~~margin-top:-28px;~~

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~~right:135px;~~

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~~line-height:56px;~~

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~~color:#fff;~~

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~~padding-left:60px;~~

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~~font-size:15px;~~

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~~background: #000 url(../images/ajax-loader.gif) no-repeat 10px 50%;~~

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~~opacity: 0.7;~~

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~~z-index:9999;~~

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~~-moz-border-radius:20px;~~

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~~-webkit-border-radius:20px;~~

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~~border-radius:20px;~~

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~~filter:progid:DXImageTransform.Microsoft.Alpha(opacity=70);~~

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}

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~~</style>~~

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~~<script>~~

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/*

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* jQuery Booklet Plugin

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*

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* Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)

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* and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.

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*

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* Version : 1.1.0

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*

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* Originally based on the work of:

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* 1) Charles Mangin (http://clickheredammit.com/pageflip/)

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*/

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~~;(function($) {~~

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~~$.fn.booklet = function(options){~~

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~~var o = $.extend({}, $.fn.booklet.defaults, options);~~

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~~return $(this).each(function()~~

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{

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~~var command, config, obj, id, i, target;~~

-

~~//option type string - api call~~

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~~if(typeof options == 'string')~~

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{

-

~~//check if booklet has been initialized~~

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~~if($(this).data('booklet')){~~

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~~command = options.toLowerCase();~~

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~~obj = $.fn.booklet.interfaces[$(this).data('id')];~~

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~~if(command == 'next'){ obj.next() }~~

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~~else if(command == 'prev'){ obj.prev() }~~

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}

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}

-

~~//option type number - api call~~

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~~else if(typeof options == 'number')~~

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{

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~~//check if booklet has been initialized~~

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~~if($(this).data('booklet')){~~

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~~target = options;~~

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~~obj = $.fn.booklet.interfaces[$(this).data('id')];~~

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~~if(target % 2 != 0) {~~

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~~target-= 1;~~

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}

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~~obj.gotoPage(target);~~

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}

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}

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~~//else build new booklet~~

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~~else~~

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{

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~~config = $.extend(true, {}, o);~~

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~~// Determine ID (Reuse array slots if possible)~~

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~~id = $.fn.booklet.interfaces.length;~~

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~~for(i = 0; i < id; i++)~~

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{

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~~if(typeof $.fn.booklet.interfaces[i] == 'undefined'){ id = i; break; }~~

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}

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~~// Instantiate the booklet~~

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~~obj = new booklet($(this), config, id);~~

-

~~// Add API references~~

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~~$.fn.booklet.interfaces[id] = obj;~~

-

}

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~~});~~

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}

-

~~function booklet(target, options, id){~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~//VARS + STRUCTURE~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~var self, opts, b, src,~~

-

~~hash, i, j, p, diff, busy, init, rhover, lhover,~~

-

~~titles = new Array(), chapters = new Array(),~~

-

~~pN, p0, p1, p2, p3, p4, pNwrap, p0wrap, p1wrap, p2wrap, p3wrap, p4wrap, wraps, sF, sB,~~

-

~~overlays, overlayN, overlayP, tabs, tabN, tabP, arrows, arrowN, arrowP, next, prev, ctrlsN, ctrlsP,~~

-

~~menu, chapter, dd, ddUL, ddH, ddLI, ddA, ddT, ddC, ddCUL, ddCH, ddCLI, ddCA, ddCT,~~

-

~~empty = '<div class="b-page-empty" title="" rel=""></div>', blank = '<div class="b-page-blank" title="" rel=""></div>'~~

-

;

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~~busy = false;~~

-

~~init = false;~~

-

~~rhover = lhover = false;~~

-

~~self = this;~~

-

~~self.options = options;~~

-

~~self.id = id;~~

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~~self.hash = '';~~

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~~opts = self.options;~~

-

~~b = target.addClass('booklet');~~

-

~~src = b.children('.b-load');~~

-

~~//save page titles and chapter names, add page numbers~~

-

~~initPages();~~

-

~~//store data for api calls~~

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~~b.data('booklet',true);~~

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~~b.data('id', id);~~

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~~b.data('total', src.children().length);~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~// SETUP OPTIONS~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~//set width + height~~

-

~~if(!opts.width){~~

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~~opts.width = b.width();~~

-

}

-

~~if(!opts.height){~~

-

~~opts.height = b.height();~~

-

}

-

~~b.width(opts.width);~~

-

~~b.height(opts.height);~~

-

~~//save page sizes and other vars~~

-

~~opts.pWidth = opts.width/2;~~

-

~~opts.pWidthN = '-'+(opts.width/2)+'px';~~

-

~~opts.pWidthH = opts.width/4;~~

-

~~opts.pHeight = opts.height;~~

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~~opts.pTotal = src.children().length;~~

-

~~opts.speedH = opts.speed/2;~~

-

~~//set startingPage~~

-

~~if(opts.direction == 'LTR'){~~

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~~opts.curr = 0;~~

-

~~}else if(opts.direction == 'RTL'){~~

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~~opts.curr = opts.pTotal-2;~~

-

}

-

~~if(!isNaN(opts.startingPage) && opts.startingPage <= opts.pTotal && opts.startingPage > 0){~~

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~~if((opts.startingPage % 2) != 0){opts.startingPage--};~~

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~~opts.curr = opts.startingPage;~~

-

}

-

~~//set booklet opts.name~~

-

~~if(opts.name){~~

-

~~document.title = opts.name;~~

-

~~}else{~~

-

~~opts.name = document.title;~~

-

}

-

~~//save shadow widths for anim~~

-

~~if(opts.shadows){~~

-

~~opts.shadowTopFwdWidth = '-'+opts.shadowTopFwdWidth+'px';~~

-

~~opts.shadowTopBackWidth = '-'+opts.shadowTopBackWidth+'px';~~

-

}

-

~~//setup menu~~

-

~~if(opts.menu){~~

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~~menu = $(opts.menu).addClass('b-menu');~~

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~~p = opts.curr;~~

-

~~//setup page selctor~~

-

~~if(opts.pageSelector){~~

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~~//add selector~~

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~~dd = $('<div class="b-selector b-selector-page"><span class="b-current">'+ (p+1) +' - '+ (p+2) +'</span></div>').appendTo(menu);~~

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~~ddUL = $('<ul></ul>').appendTo(dd).empty().css('height','auto');~~

-

~~//loop through all pages~~

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~~for(i=0; i < opts.pTotal; i+=2){~~

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~~j = i;~~

-

~~//nums for normal view~~

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~~nums = (j+1) +'-'+ (j+2);~~

-

~~if(opts.closed){~~

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~~//nums for closed book~~

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~~j--;~~

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~~if(i==0){nums='1'}~~

-

~~else if(i==opts.pTotal-2){nums=opts.pTotal-2}~~

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~~else {nums = (j+1) +'-'+ (j+2);}~~

-

~~//nums for closed book with covers~~

-

~~if(opts.covers){~~

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~~j--;~~

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~~if(i==0){nums=''}~~

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~~else if(i==opts.pTotal-2){nums=''}~~

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~~else {nums = (j+1) +'-'+ (j+2);}~~

-

}

-

}

-

~~//nums for RTL direction~~

-

~~if(opts.direction == 'RTL'){~~

-

~~nums = (Math.abs(j - opts.pTotal)-1) +' - '+ ((Math.abs(j - opts.pTotal)));~~

-

~~if(opts.closed){~~

-

~~if(i==opts.pTotal-2){nums='1'}~~

-

~~else if(i==0){nums=opts.pTotal-2}~~

-

~~else{nums = (Math.abs(j - opts.pTotal)-3) +' - '+ ((Math.abs(j - opts.pTotal)-2));}~~

-

~~if(opts.covers){~~

-

~~if(i==opts.pTotal-2){nums=''}~~

-

~~else if(i==0){nums=''}~~

-

~~else{nums = (Math.abs(j - opts.pTotal)-5) +' - '+ ((Math.abs(j - opts.pTotal)-4));}~~

-

}

-

}

-

~~dd.find('.b-current').text(nums);~~

-

~~ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i+1] +'</span><span class="b-num">'+ nums +'</span></a></li>').prependTo(ddUL);~~

-

~~}else{~~

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~~if(i==0){dd.find('.b-current').text(nums);}~~

-

~~ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i] +'</span><span class="b-num">'+ nums +'</span></a></li>').appendTo(ddUL);~~

-

}

-

~~ddA = ddLI.find('a');~~

-

~~if(!opts.hash){~~

-

~~ddA.click(function(){~~

-

~~if(opts.direction == 'RTL'){dd.find('.b-current').text($(this).find('.b-num').text());}~~

-

~~ddT = parseInt($(this).attr('id').replace('selector-page-',''));~~

-

~~self.gotoPage(ddT);~~

-

~~return false;~~

-

~~});~~

-

}

-

}

-

~~//set height~~

-

~~ddH = ddUL.height();~~

-

~~ddUL.css({'height':0, 'padding-bottom':0});~~

-

~~//add hover effects~~

-

~~dd.unbind('hover').hover(function(){~~

-

~~ddUL.stop().animate({height:ddH, paddingBottom:10}, 500);~~

-

~~},function(){~~

-

~~ddUL.stop().animate({height:0, paddingBottom:0}, 500);~~

-

~~});~~

-

}

-

~~//setup chapter selctor~~

-

~~if(opts.chapterSelector){~~

-

~~chapter = chapters[opts.curr];~~

-

~~if(chapter == ""){ chapter = chapters[opts.curr+1]; }~~

-

~~ddC = $('<div class="b-selector b-selector-chapter"><span class="b-current">'+chapter+'</span></div>').appendTo(menu);~~

-

~~ddCUL = $('<ul></ul>').appendTo(ddC).empty().css('height','auto');~~

-

~~for(i=0; i < opts.pTotal; i+=1){~~

-

~~if(chapters[i] != "" && typeof chapters[i] != "undefined"){~~

-

~~if(opts.direction == 'RTL'){~~

-

~~j = i;~~

-

~~if(j % 2 != 0){j--;}~~

-

~~ddC.find('.b-current').text(chapters[i]);~~

-

~~ddCLI = $('<li><a href="#/page/'+ (j+1) +'" id="selector-page-'+(j)+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').prependTo(ddCUL);~~

-

~~}else{~~

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~~ddCLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').appendTo(ddCUL);~~

-

}

-

~~ddCA = ddCLI.find('a');~~

-

~~if(!opts.hash){~~

-

~~ddCA.click(function(){~~

-

~~if(opts.direction == 'RTL'){ddC.find('.b-current').text($(this).find('.b-text').text());}~~

-

~~ddCT = parseInt($(this).attr('id').replace('selector-page-',''));~~

-

~~self.gotoPage(ddCT);~~

-

~~return false;~~

-

~~});~~

-

}

-

}

-

}

-

~~ddCH = ddCUL.height();~~

-

~~ddCUL.css({'height':0, 'padding-bottom':0});~~

-

~~ddC.unbind('hover').hover(function(){~~

-

~~ddCUL.stop().animate({height:ddCH, paddingBottom:10}, 500);~~

-

~~},function(){~~

-

~~ddCUL.stop().animate({height:0, paddingBottom:0}, 500);~~

-

~~});~~

-

}

-

}

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~// API METHODS~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~$.extend(self,~~

-

{

-

~~next : function(){~~

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~~if(!busy){~~

-

~~self.gotoPage(opts.curr+2);~~

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}

-

},

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~~prev : function(){~~

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~~if(!busy){~~

-

~~self.gotoPage(opts.curr-2);~~

-

}

-

},

-

~~gotoPage : function(num){~~

-

~~//moving forward (increasing number)~~

-

~~if(num > opts.curr && num < opts.pTotal && num >= 0 && !busy){~~

-

~~busy = true;~~

-

~~diff = num - opts.curr;~~

-

~~opts.curr = num;~~

-

~~opts.before.call(self, opts);~~

-

~~updatePager();~~

-

~~updateCtrls();~~

-

~~updateHash(opts.curr+1, opts);~~

-

~~initAnim(diff, true, sF);~~

-

~~//hide p2 as p3 moves across it~~

-

~~p2.stop().animate({width:0}, opts.speedH, opts.easeIn);~~

-

~~//animate p3 from right to left (left: movement, width: reveal slide, paddingLeft: shadow underneath)~~

-

~~//call setuppages at end of animation to reset pages~~

-

~~p3.stop().animate({left:opts.pWidthH, width:opts.pWidthH, paddingLeft: opts.shadowBtmWidth}, opts.speedH, opts.easeIn)~~

-

~~.animate({left:0, width:opts.pWidth, paddingLeft:0}, opts.speedH);~~

-

~~p3wrap.animate({left:opts.shadowBtmWidth}, opts.speedH, opts.easeIn)~~

-

~~.animate({left:0}, opts.speedH, opts.easeOut, function(){updateAfter()});~~

-

~~//moving backward (decreasing number)~~

-

~~}else if(num < opts.curr && num < opts.pTotal && num >= 0 && !busy){~~

-

~~busy = true;~~

-

~~diff = opts.curr - num;~~

-

~~opts.curr = num;~~

-

~~opts.before.call(self, opts);~~

-

~~updatePager();~~

-

~~updateCtrls();~~

-

~~updateHash(opts.curr+1, opts);~~

-

~~initAnim(diff, false, sB);~~

-

~~//hide p1 as p0 moves across it~~

-

~~p1.animate({left:opts.pWidth, width:0}, opts.speed, opts.easing);~~

-

~~p1wrap.animate({left:opts.pWidthN}, opts.speed, opts.easing);~~

-

~~//animate p0 from left to right (right: movement, width: reveal slide, paddingLeft: shadow underneath)~~

-

~~p0.animate({left:opts.pWidthH, width:opts.pWidthH}, opts.speedH, opts.easeIn)~~

-

~~.animate({left:opts.pWidth, width:opts.pWidth}, opts.speedH, opts.easeOut);~~

-

~~//animate .wrapper content with p0 to keep content right aligned throughout~~

-

~~//call setuppages at end of animation to reset pages~~

-

~~p0wrap.animate({right:opts.shadowBtmWidth}, opts.speedH,opts. easeIn)~~

-

~~.animate({right:0}, opts.speedH, opts.easeOut, function(){updateAfter()});~~

-

}

-

}

-

~~});~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~// SETUP CONTROLS~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~//add prev next user defined controls~~

-

~~if(opts.next){~~

-

~~next = $(opts.next);~~

-

~~next.click(function(e){e.preventDefault(); self.next();});~~

-

}

-

~~if(opts.prev){~~

-

~~prev = $(opts.prev);~~

-

~~prev.click(function(e){e.preventDefault(); self.prev();});~~

-

}

-

~~//add overlays~~

-

~~if(opts.overlays){~~

-

~~overlayP = $('<div class="b-overlay b-overlay-prev b-prev" title="Previous Page"></div>').appendTo(b);~~

-

~~overlayN = $('<div class="b-overlay b-overlay-next b-next" title="Next Page"></div>').appendTo(b);~~

-

~~overlays = b.find('.b-overlay');~~

-

~~if ($.browser.msie) {~~

-

~~overlays.css({'background':'#fff','filter':'progid:DXImageTransform.Microsoft.Alpha(opacity=0) !important'});~~

-

}

-

}

-

~~//add tabs~~

-

~~if(opts.tabs){~~

-

~~tabP = $('<div class="b-tab b-tab-prev b-prev" title="Previous Page">Previous</div>').appendTo(b);~~

-

~~tabN = $('<div class="b-tab b-tab-next b-next" title="Next Page">Next</div>').appendTo(b);~~

-

~~tabs = b.find('.b-tab');~~

-

~~if(opts.tabWidth){~~

-

~~tabs.width(opts.tabWidth);~~

-

}

-

~~if(opts.tabHeight){~~

-

~~tabs.height(opts.tabHeight);~~

-

}

-

~~tabs.css({'top': '-'+tabN.outerHeight()+'px'});~~

-

~~b.css({'marginTop': tabN.outerHeight()});~~

-

~~//update ctrls for RTL direction~~

-

~~if(opts.direction == 'RTL'){~~

-

~~tabN.html('Previous').attr('title','Previous Page');~~

-

~~tabP.html('Next').attr('title','Next Page');~~

-

}

-

~~}else{~~

-

~~b.css({'marginTop': 0});~~

-

}

-

~~//add arrows~~

-

~~if(opts.arrows){~~

-

~~arrowP = $('<div class="b-arrow b-arrow-prev b-prev" title="Previous Page"><div>Previous</div></div>').appendTo(b);~~

-

~~arrowN = $('<div class="b-arrow b-arrow-next b-next" title="Next Page"><div>Next</div></div>').appendTo(b);~~

-

~~arrows = b.find('.b-arrow');~~

-

~~//update ctrls for RTL direction~~

-

~~if(opts.direction == 'RTL'){~~

-

~~arrowN.html('<div>Previous</div>').attr('title','Previous Page');~~

-

~~arrowP.html('<div>Next</div>').attr('title','Next Page');~~

-

}

-

}

-

~~//save all "b-prev" and "b-next" controls~~

-

~~ctrlsN = b.find('.b-next');~~

-

~~ctrlsP = b.find('.b-prev');~~

-

~~//add click actions~~

-

~~ctrlsN.click(function(e){e.preventDefault(); self.next();});~~

-

~~ctrlsP.click(function(e){e.preventDefault(); self.prev();});~~

-

~~//add page hover animations~~

-

~~if(opts.hovers){~~

-

~~ctrlsN.hover(~~

-

~~function(){~~

-

~~if(!busy && opts.curr+2 <= opts.pTotal-2){~~

-

~~//animate~~

-

~~p2.stop().animate({'width':opts.pWidth-40}, 500, opts.easing);~~

-

~~p3.stop().animate({'left':opts.width-40, 'width':20, paddingLeft: 10}, 500, opts.easing);~~

-

~~rhover = true;~~

-

}

-

},

-

~~function(){~~

-

~~if(!busy && opts.curr+2 <= opts.pTotal-2){~~

-

~~p2.stop().animate({'width':opts.pWidth}, 500, opts.easing);~~

-

~~p3.stop().animate({'left':opts.width, 'width':0, paddingLeft: 0}, 500, opts.easing);~~

-

~~rhover = false;~~

-

}

-

}

-

);

-

~~ctrlsP.hover(~~

-

~~function(){~~

-

~~if(!busy && opts.curr-2 >= 0){~~

-

~~//animate~~

-

~~p1.stop().animate({left:10, width:opts.pWidth-10}, 400, opts.easing);~~

-

~~p1wrap.stop().animate({left:"-10px"}, 400, opts.easing);~~

-

~~p0.stop().animate({left:10, width:40}, 400, opts.easing);~~

-

~~p0wrap.stop().animate({right:10}, 400, opts.easing);~~

-

~~lhover = true;~~

-

}

-

},

-

~~function(){~~

-

~~if(!busy && opts.curr-2 >= 0){~~

-

~~p1.stop().animate({left:0, width:opts.pWidth}, 400, opts.easing);~~

-

~~p1wrap.stop().animate({left:0}, 400, opts.easing);~~

-

~~p0.stop().animate({left:0, width:0}, 400, opts.easing);~~

-

~~p0wrap.stop().animate({right:0}, 400, opts.easing);~~

-

~~lhover = false;~~

-

}

-

}

-

);

-

}

-

~~//arrow animations~~

-

~~if(opts.arrows){~~

-

~~if($.support.opacity){~~

-

~~ctrlsN.hover(~~

-

~~function(){arrowN.find('div').stop().fadeTo('fast', 1);},~~

-

~~function(){arrowN.find('div').stop().fadeTo('fast', 0);~~

-

~~});~~

-

~~ctrlsP.hover(~~

-

~~function(){arrowP.find('div').stop().fadeTo('fast', 1);},~~

-

~~function(){arrowP.find('div').stop().fadeTo('fast', 0);~~

-

~~});~~

-

~~}else{~~

-

~~ctrlsN.hover(~~

-

~~function(){arrowN.find('div').show();},~~

-

~~function(){arrowN.find('div').hide();~~

-

~~});~~

-

~~ctrlsP.hover(~~

-

~~function(){arrowP.find('div').show();},~~

-

~~function(){arrowP.find('div').hide();~~

-

~~});~~

-

}

-

}

-

~~//keyboard ctrls~~

-

~~if(opts.keyboard){~~

-

~~//keyboard ctrls~~

-

~~$(document).keyup(function(event){~~

-

~~if(event.keyCode == 37){self.prev();}~~

-

~~else if(event.keyCode == 39){self.next();}~~

-

~~});~~

-

}

-

~~//hash ctrls~~

-

~~if(opts.hash){~~

-

~~setupHash();~~

-

~~clearInterval();~~

-

~~setInterval(function(){pollHash()}, 250);~~

-

}

-

~~//first time setup~~

-

~~resetPages();~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~//General Functions~~

-

~~///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////~~

-

~~function initPages(){~~

-

~~//fix for odd number of pages~~

-

~~if((src.children().length % 2) != 0){~~

-

~~//if book is closed and using covers, add page before back cover, else after last page~~

-

~~if(opts.closed && opts.covers){~~

-

~~src.children().last().before(blank);~~

-

~~}else{~~

-

~~src.children().last().after(blank);~~

-

}

-

}

-

~~//if closed book, add empty pages to start and end~~

-

~~if(opts.closed){~~

-

~~$(empty).attr({'title':opts.closedFrontTitle || "Beginning", 'rel':opts.closedFrontChapter || "Beginning of Book"}).prependTo(src);~~

-

~~src.children().last().attr({'title':opts.closedBackTitle || "End", 'rel':opts.closedBackChapter || "End of Book"});~~

-

~~src.append(empty);~~

-

}

-

~~if(opts.direction == 'LTR'){~~

-

~~j = 0;~~

-

~~}else{~~

-

~~j = src.children().length;~~

-

~~if(opts.closed){j-=2;}~~

-

~~if(opts.covers){j-=2;}~~

-

~~$(src.children().get().reverse()).each(function(){~~

-

~~$(this).appendTo(src);~~

-

~~});~~

-

}

-

~~//save titles and chapters~~

-

~~src.children().each(function(i){~~

-

~~//save chapter title~~

-

~~if($(this).attr('rel')){~~

-

~~chapters[i] = $(this).attr('rel');~~

-

~~}else{~~

-

~~chapters[i] = "";~~

-

}

-

~~//save page title~~

-

~~titles[i] = $(this).attr('title');~~

-

~~//give content the correct wrapper and page wrapper~~

-

~~if($(this).hasClass('b-page-empty')){~~

-

~~$(this).wrap('<div class="b-page"><div class="b-wrap"></div></div>');~~

-

~~}else if(opts.closed && opts.covers && (i == 1 || i == src.children().length-2)){~~

-

~~$(this).wrap('<div class="b-page"><div class="b-wrap b-page-cover"></div></div>');~~

-

~~}else if(i % 2 != 0){~~

-

~~$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-right"></div></div>');~~

-

~~}else{~~

-

~~$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-left"></div></div>');~~

-

}

-

~~$(this).parents('.b-page').addClass('b-page-'+i);~~

-

~~//add page numbers~~

-

~~if(opts.pageNumbers && !$(this).hasClass('b-page-empty') && (!opts.closed || (opts.closed && !opts.covers) || (opts.closed && opts.covers && i != 1 && i != src.children().length-2))){~~

-

~~if(opts.direction == 'LTR'){j++;}~~

-

~~$(this).parent().append('<div class="b-counter">'+(j)+'</div>');~~

-

~~if(opts.direction == 'RTL'){j--;}~~

-

}

-

~~});~~

-

}

-

~~function resetPages(){~~

-

~~//reset all content~~

-

~~b.find('.b-page').removeClass('b-pN b-p0 b-p1 b-p2 b-p3 b-p4').hide();~~

-

~~if(init){~~

-

~~j = opts.pTotal-1;~~

-

~~for(i=0;i<opts.pTotal;i++){~~

-

~~b.find('.b-page-'+i).detach().appendTo(src);~~

-

}

-

}

-

~~//add page classes~~

-

~~if(opts.curr-2 >= 0){~~

-

~~b.find('.b-page-'+(opts.curr-2)).addClass('b-pN').show();~~

-

~~b.find('.b-page-'+(opts.curr-1)).addClass('b-p0').show();~~

-

}

-

~~b.find('.b-page-'+(opts.curr)).addClass('b-p1').show();~~

-

~~b.find('.b-page-'+(opts.curr+1)).addClass('b-p2').show();~~

-

~~if(opts.curr+3 <= opts.pTotal){~~

-

~~b.find('.b-page-'+(opts.curr+2)).addClass('b-p3').show();~~

-

~~b.find('.b-page-'+(opts.curr+3)).addClass('b-p4').show();~~

-

}

-

~~//save structure elems to vars~~

-

~~pN = b.find('.b-pN');~~

-

~~p0 = b.find('.b-p0');~~

-

~~p1 = b.find('.b-p1');~~

-

~~p2 = b.find('.b-p2');~~

-

~~p3 = b.find('.b-p3');~~

-

~~p4 = b.find('.b-p4');~~

-

~~pNwrap = b.find('.b-pN .b-wrap');~~

-

~~p0wrap = b.find('.b-p0 .b-wrap');~~

-

~~p1wrap = b.find('.b-p1 .b-wrap');~~

-

~~p2wrap = b.find('.b-p2 .b-wrap');~~

-

~~p3wrap = b.find('.b-p3 .b-wrap');~~

-

~~p4wrap = b.find('.b-p4 .b-wrap');~~

-

~~wraps = b.find('.b-wrap');~~

-

~~//update css~~

-

~~wraps.attr('style','');~~

-

~~wraps.css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});~~

-

~~p1.css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~p2.css({'left':opts.pWidth, 'width':opts.pWidth, 'opacity':1, 'height':opts.pHeight});~~

-

~~pN.css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~p0.css({'left':0, 'width':0, 'height':opts.pHeight});~~

-

~~p3.stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});~~

-

~~p3wrap.stop().css({'left':0});~~

-

~~p4.css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~//update page order for animations~~

-

~~if(opts.curr+3 <= opts.pTotal){~~

-

~~p3.after(p0.detach());~~

-

~~p1.after(p4.detach());~~

-

~~}else{~~

-

~~p0.detach().appendTo(src);~~

-

}

-

~~init = true;~~

-

~~//add shadows~~

-

~~sF = sB = null;~~

-

~~b.find('.b-shadow-b, .b-shadow-f').remove();~~

-

~~if(opts.shadows){~~

-

~~sF = $('<div class="b-shadow-f"></div>').appendTo(p3).css({'right':0,'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~sB = $('<div class="b-shadow-b"></div>').appendTo(p0).css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});~~

-

}

-

}

-

~~function initAnim(diff, inc, shadow){~~

-

~~//setup content~~

-

~~if(inc && diff > 2){~~

-

~~b.find('.b-p3, .b-p4').removeClass('b-p3 b-p4').hide();~~

-

~~b.find('.b-page-'+opts.curr).addClass('b-p3').show().stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});~~

-

~~b.find('.b-page-'+(opts.curr+1)).addClass('b-p4').show().css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});~~

-

~~b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});~~

-

~~p3 = b.find('.b-p3');~~

-

~~p4 = b.find('.b-p4');~~

-

~~p3wrap = b.find('.b-p3 .b-wrap');~~

-

~~p4wrap = b.find('.b-p4 .b-wrap');~~

-

~~if(rhover){~~

-

~~p3.css({'left':opts.width-40, 'width':20, 'padding-left': 10});~~

-

}

-

~~shadow.appendTo(p3);~~

-

~~p1.after(p4.detach());~~

-

~~p2.after(p3.detach());~~

-

~~}else if(!inc && diff > 2){~~

-

~~b.find('.b-pN, .b-p0').removeClass('b-pN b-p0').hide();~~

-

~~b.find('.b-page-'+opts.curr).addClass('b-pN').show().css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});~~

-

~~b.find('.b-page-'+(opts.curr+1)).addClass('b-p0').show().css({'left':0, 'width':0, 'height':opts.pHeight});~~

-

~~b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});~~

-

~~b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});~~

-

~~pN = b.find('.b-pN');~~

-

~~p0 = b.find('.b-p0');~~

-

~~pNwrap = b.find('.b-pN .b-wrap');~~

-

~~p0wrap = b.find('.b-p0 .b-wrap');~~

-

~~if(lhover){~~

-

~~p0.css({left:10, width:40});~~

-

~~p0wrap.css({right:10});~~

-

}

-

~~shadow.appendTo(p0);~~

-

~~p0.detach().appendTo(src);~~

-

}

-

~~//updates if moving to start and end of book~~

-

~~if(opts.closed){~~

-

~~if(!inc && opts.curr == 0){~~

-

~~pN.hide();~~

-

~~}else if(!inc){~~

-

~~pN.show();~~

-

}

-

~~if(inc && opts.curr >= opts.pTotal-2){~~

-

~~p4.hide();~~

-

~~}else if(inc){~~

-

~~p4.show();~~

-

}

-

}

-

~~//init shadows~~

-

~~if(opts.shadows){~~

-

~~//check for opacity support -> animate shadow overlay on moving slide~~

-

~~if($.support.opacity){~~

-

~~shadow.animate({opacity:1}, opts.speedH, opts.easeIn)~~

-

~~.animate({opacity:0}, opts.speedH, opts.easeOut);~~

-

~~}else{~~

-

~~if(inc){~~

-

~~shadow.animate({right:opts.shadowTopFwdWidth}, opts.speed, opts.easeIn);~~

-

~~}else{~~

-

~~shadow.animate({left:opts.shadowTopBackWidth}, opts.speed, opts.easeIn);~~

-

}

-

}

-

}

-

}

-

~~function updateAfter(){~~

-

~~resetPages();~~

-

~~updatePager();~~

-

~~updateCtrls();~~

-

~~opts.after.call(self, opts);~~

-

~~busy = false;~~

-

}

-

~~function updateCtrls(){~~

-

~~//update ctrls, cursors and visibility~~

-

~~if(opts.overlays || opts.tabs || opts.arrows){~~

-

~~if(opts.curr < opts.pTotal-2){~~

-

~~ctrlsN.fadeIn('fast').css('cursor',opts.cursor);~~

-

~~}else{~~

-

~~ctrlsN.fadeOut('fast').css('cursor','default');~~

-

}

-

~~if(opts.curr >= 2 && opts.curr != 0){~~

-

~~ctrlsP.fadeIn('fast').css('cursor',opts.cursor);~~

-

~~}else{~~

-

~~ctrlsP.fadeOut('fast').css('cursor','default');~~

-

}

-

}

-

}

-

~~function updatePager(){~~

-

~~if(opts.pageSelector){~~

-

~~if(opts.direction == 'RTL'){~~

-

~~nums = (Math.abs(opts.curr - opts.pTotal)-1) +' - '+ ((Math.abs(opts.curr - opts.pTotal)));~~

-

~~if(opts.closed){~~

-

~~if(opts.curr==opts.pTotal-2){nums='1'}~~

-

~~else if(opts.curr==0){nums=opts.pTotal-2}~~

-

~~else{nums = (Math.abs(opts.curr - opts.pTotal)-2) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-1));}~~

-

~~if(opts.covers){~~

-

~~if(opts.curr==opts.pTotal-2){nums=''}~~

-

~~else if(opts.curr==0){nums=''}~~

-

~~else{nums = (Math.abs(opts.curr - opts.pTotal)-3) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-2));}~~

-

}

-

}

-

~~$(opts.menu+' .b-selector-page .b-current').text(nums);~~

-

~~}else{~~

-

~~nums = (opts.curr+1) +' - '+ (opts.curr+2);~~

-

~~if(opts.closed){~~

-

~~if(opts.curr==0){nums='1'}~~

-

~~else if(opts.curr==opts.pTotal-2){nums=opts.pTotal-2}~~

-

~~else {nums = (opts.curr) +'-'+ (opts.curr+1);}~~

-

~~if(opts.covers){~~

-

~~if(opts.curr==0){nums=''}~~

-

~~else if(opts.curr==opts.pTotal-2){nums=''}~~

-

~~else {nums = (opts.curr-1) +'-'+ (opts.curr);}~~

-

}

-

}

-

~~$(opts.menu+' .b-selector-page .b-current').text(nums);~~

-

}

-

}

-

~~if(opts.chapterSelector){~~

-

~~if(chapters[opts.curr]!=""){~~

-

~~$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);~~

-

~~}else if(chapters[opts.curr+1]!=""){~~

-

~~$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);~~

-

}

-

~~if(opts.direction == 'RTL' && chapters[opts.curr+1]!=""){~~

-

~~$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);~~

-

~~}else if(chapters[opts.curr]!=""){~~

-

~~$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);~~

-

}

-

}

-

}

-

~~function setupHash(){~~

-

~~hash = getHashNum();~~

-

~~if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0 && hash != ''){~~

-

~~if((hash % 2) != 0){~~

-

~~hash--;~~

-

}

-

~~opts.curr = hash;~~

-

~~}else{~~

-

~~updateHash(opts.curr+1, opts);~~

-

}

-

~~self.hash = hash;~~

-

}

-

~~function pollHash(){~~

-

~~hash = getHashNum();~~

-

~~//check page num~~

-

~~if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0){~~

-

~~if(hash != opts.curr && hash.toString()!=self.hash){~~

-

~~if((hash % 2) != 0){hash--};~~

-

~~document.title = opts.name + " - Page "+ (hash+1);~~

-

~~if(!busy){~~

-

~~self.gotoPage(hash);~~

-

~~self.hash = hash;~~

-

}

-

}

-

}

-

}

-

~~//get page number from hash tag, last element~~

-

~~function getHashNum(){~~

-

~~var hash = window.location.hash.split('/');~~

-

~~if(hash.length > 1){~~

-

~~return parseInt(hash[2])-1;~~

-

~~}else{~~

-

~~return '';~~

-

}

-

}

-

~~//set the hash~~

-

~~function updateHash(hash, opts){~~

-

~~if(opts.hash){~~

-

~~window.location.hash = "/page/" + hash;~~

-

}

-

}

-

}

-

~~//define empty array to hold API references~~

-

~~$.fn.booklet.interfaces = [];~~

-

~~//define default options~~

-

~~$.fn.booklet.defaults = {~~

-

~~name: null, // name of the booklet to display in the document title bar~~

-

~~width: 600, // container width~~

-

~~height: 400, // container height~~

-

~~speed: 1000, // speed of the transition between pages~~

-

~~direction: 'LTR', // direction of the overall content organization, default LTR, left to right, can be RTL for languages which read right to left~~

-

~~startingPage: 0, // index of the first page to be displayed~~

-

~~easing: 'easeInOutQuad', // easing method for complete transition~~

-

~~easeIn: 'easeInQuad', // easing method for first half of transition~~

-

~~easeOut: 'easeOutQuad', // easing method for second half of transition~~

-

~~closed: false, // start with the book "closed", will add empty pages to beginning and end of book~~

-

~~closedFrontTitle: null, // used with "closed", "menu" and "pageSelector", determines title of blank starting page~~

-

~~closedFrontChapter: null, // used with "closed", "menu" and "chapterSelector", determines chapter name of blank starting page~~

-

~~closedBackTitle: null, // used with "closed", "menu" and "pageSelector", determines chapter name of blank ending page~~

-

~~closedBackChapter: null, // used with "closed", "menu" and "chapterSelector", determines chapter name of blank ending page~~

-

~~covers: false, // used with "closed", makes first and last pages into covers, without page numbers (if enabled)~~

-

~~pagePadding: 10, // padding for each page wrapper~~

-

~~pageNumbers: true, // display page numbers on each page~~

-

~~hovers: true, // enables preview pageturn hover animation, shows a small preview of previous or next page on hover~~

-

~~overlays: true, // enables navigation using a page sized overlay, when enabled links inside the content will not be clickable~~

-

~~tabs: false, // adds tabs along the top of the pages~~

-

~~tabWidth: 60, // set the width of the tabs~~

-

~~tabHeight: 20, // set the height of the tabs~~

-

~~arrows: false, // adds arrows overlayed over the book edges~~

-

~~cursor: 'pointer', // cursor css setting for side bar areas~~

-

~~hash: false, // enables navigation using a hash string, ex: #/page/1 for page 1, will affect all booklets with 'hash' enabled~~

-

~~keyboard: true, // enables navigation with arrow keys (left: previous, right: next)~~

-

~~next: null, // selector for element to use as click trigger for next page~~

-

~~prev: null, // selector for element to use as click trigger for previous page~~

-

~~menu: null, // selector for element to use as the menu area, required for 'pageSelector'~~

-

~~pageSelector: false, // enables navigation with a dropdown menu of pages, requires 'menu'~~

-

~~chapterSelector: false, // enables navigation with a dropdown menu of chapters, determined by the "rel" attribute, requires 'menu'~~

-

~~shadows: true, // display shadows on page animations~~

-

~~shadowTopFwdWidth: 166, // shadow width for top forward anim~~

-

~~shadowTopBackWidth: 166, // shadow width for top back anim~~

-

~~shadowBtmWidth: 50, // shadow width for bottom shadow~~

-

~~before: function(){}, // callback invoked before each page turn animation~~

-

~~after: function(){} // callback invoked after each page turn animation~~

-

}

-

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/*

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* jQuery Booklet Plugin

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*

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* Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)

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* and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.

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*

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* Version : 1.1.0

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*

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* Originally based on the work of:

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* 1) Charles Mangin (http://clickheredammit.com/pageflip/)

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*/

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*

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* TERMS OF USE - jQuery Easing

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*

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* Open source under the BSD License.

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*

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*

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* Redistribution and use in source and binary forms, with or without modification,

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* are permitted provided that the following conditions are met:

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*

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* Redistributions of source code must retain the above copyright notice, this list of

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* conditions and the following disclaimer.

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* of conditions and the following disclaimer in the documentation and/or other materials

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* provided with the distribution.

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*

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* Neither the name of the author nor the names of contributors may be used to endorse

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*

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* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY

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* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF

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* COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,

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* EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE

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* GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED

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*

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*/

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~~/*!~~

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* jQuery JavaScript Library v1.4.4

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* http://jquery.com/

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*

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* Includes Sizzle.js

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* http://sizzlejs.com/

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*

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* Date: Thu Nov 11 19:04:53 2010 -0500

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[b,a])},handleComplete:function(a,b,d){a.complete&&a.complete.call(a.context,b,d);a.global&&c.triggerGlobal(a,"ajaxComplete",[b,a]);a.global&&c.active--===1&&c.event.trigger("ajaxStop")},triggerGlobal:function(a,b,d){(a.context&&a.context.url==null?c(a.context):c.event).trigger(b,d)},httpSuccess:function(a){try{return!a.status&&location.protocol==="file:"||a.status>=200&&a.status<300||a.status===304||a.status===1223}catch(b){}return false},httpNotModified:function(a,b){var d=a.getResponseHeader("Last-Modified"),

-

e=a.getResponseHeader("Etag");if(d)c.lastModified[b]=d;if(e)c.etag[b]=e;return a.status===304},httpData:function(a,b,d){var e=a.getResponseHeader("content-type")||"",f=b==="xml"||!b&&e.indexOf("xml")>=0;a=f?a.responseXML:a.responseText;f&&a.documentElement.nodeName==="parsererror"&&c.error("parsererror");if(d&&d.dataFilter)a=d.dataFilter(a,b);if(typeof a==="string")if(b==="json"||!b&&e.indexOf("json")>=0)a=c.parseJSON(a);else if(b==="script"||!b&&e.indexOf("javascript")>=0)c.globalEval(a);return a}});

-

if(E.ActiveXObject)c.ajaxSettings.xhr=function(){if(E.location.protocol!=="file:")try{return new E.XMLHttpRequest}catch(a){}try{return new E.ActiveXObject("Microsoft.XMLHTTP")}catch(b){}};c.support.ajax=!!c.ajaxSettings.xhr();var ea={},vb=/^(?:toggle|show|hide)$/,wb=/^([+\-]=)?([\d+.\-]+)(.*)$/,ba,pa=[["height","marginTop","marginBottom","paddingTop","paddingBottom"],["width","marginLeft","marginRight","paddingLeft","paddingRight"],["opacity"]];c.fn.extend({show:function(a,b,d){if(a||a===0)return this.animate(S("show",

-

3),a,b,d);else{d=0;for(var e=this.length;d<e;d++){a=this[d];b=a.style.display;if(!c.data(a,"olddisplay")&&b==="none")b=a.style.display="";b===""&&c.css(a,"display")==="none"&&c.data(a,"olddisplay",qa(a.nodeName))}for(d=0;d<e;d++){a=this[d];b=a.style.display;if(b===""||b==="none")a.style.display=c.data(a,"olddisplay")||""}return this}},hide:function(a,b,d){if(a||a===0)return this.animate(S("hide",3),a,b,d);else{a=0;for(b=this.length;a<b;a++){d=c.css(this[a],"display");d!=="none"&&c.data(this[a],"olddisplay",

-

d)}for(a=0;a<b;a++)this[a].style.display="none";return this}},_toggle:c.fn.toggle,toggle:function(a,b,d){var e=typeof a==="boolean";if(c.isFunction(a)&&c.isFunction(b))this._toggle.apply(this,arguments);else a==null||e?this.each(function(){var f=e?a:c(this).is(":hidden");c(this)[f?"show":"hide"]()}):this.animate(S("toggle",3),a,b,d);return this},fadeTo:function(a,b,d,e){return this.filter(":hidden").css("opacity",0).show().end().animate({opacity:b},a,d,e)},animate:function(a,b,d,e){var f=c.speed(b,

-

d,e);if(c.isEmptyObject(a))return this.each(f.complete);return this[f.queue===false?"each":"queue"](function(){var h=c.extend({},f),l,k=this.nodeType===1,o=k&&c(this).is(":hidden"),x=this;for(l in a){var r=c.camelCase(l);if(l!==r){a[r]=a[l];delete a[l];l=r}if(a[l]==="hide"&&o||a[l]==="show"&&!o)return h.complete.call(this);if(k&&(l==="height"||l==="width")){h.overflow=[this.style.overflow,this.style.overflowX,this.style.overflowY];if(c.css(this,"display")==="inline"&&c.css(this,"float")==="none")if(c.support.inlineBlockNeedsLayout)if(qa(this.nodeName)===

-

"inline")this.style.display="inline-block";else{this.style.display="inline";this.style.zoom=1}else this.style.display="inline-block"}if(c.isArray(a[l])){(h.specialEasing=h.specialEasing||{})[l]=a[l][1];a[l]=a[l][0]}}if(h.overflow!=null)this.style.overflow="hidden";h.curAnim=c.extend({},a);c.each(a,function(A,C){var J=new c.fx(x,h,A);if(vb.test(C))J[C==="toggle"?o?"show":"hide":C](a);else{var w=wb.exec(C),I=J.cur()||0;if(w){var L=parseFloat(w[2]),g=w[3]||"px";if(g!=="px"){c.style(x,A,(L||1)+g);I=(L||

-

1)/J.cur()*I;c.style(x,A,I+g)}if(w[1])L=(w[1]==="-="?-1:1)*L+I;J.custom(I,L,g)}else J.custom(I,C,"")}});return true})},stop:function(a,b){var d=c.timers;a&&this.queue([]);this.each(function(){for(var e=d.length-1;e>=0;e--)if(d[e].elem===this){b&&d[e](true);d.splice(e,1)}});b||this.dequeue();return this}});c.each({slideDown:S("show",1),slideUp:S("hide",1),slideToggle:S("toggle",1),fadeIn:{opacity:"show"},fadeOut:{opacity:"hide"},fadeToggle:{opacity:"toggle"}},function(a,b){c.fn[a]=function(d,e,f){return this.animate(b,

-

d,e,f)}});c.extend({speed:function(a,b,d){var e=a&&typeof a==="object"?c.extend({},a):{complete:d||!d&&b||c.isFunction(a)&&a,duration:a,easing:d&&b||b&&!c.isFunction(b)&&b};e.duration=c.fx.off?0:typeof e.duration==="number"?e.duration:e.duration in c.fx.speeds?c.fx.speeds[e.duration]:c.fx.speeds._default;e.old=e.complete;e.complete=function(){e.queue!==false&&c(this).dequeue();c.isFunction(e.old)&&e.old.call(this)};return e},easing:{linear:function(a,b,d,e){return d+e*a},swing:function(a,b,d,e){return(-Math.cos(a*

-

Math.PI)/2+0.5)*e+d}},timers:[],fx:function(a,b,d){this.options=b;this.elem=a;this.prop=d;if(!b.orig)b.orig={}}});c.fx.prototype={update:function(){this.options.step&&this.options.step.call(this.elem,this.now,this);(c.fx.step[this.prop]||c.fx.step._default)(this)},cur:function(){if(this.elem[this.prop]!=null&&(!this.elem.style||this.elem.style[this.prop]==null))return this.elem[this.prop];var a=parseFloat(c.css(this.elem,this.prop));return a&&a>-1E4?a:0},custom:function(a,b,d){function e(l){return f.step(l)}

-

var f=this,h=c.fx;this.startTime=c.now();this.start=a;this.end=b;this.unit=d||this.unit||"px";this.now=this.start;this.pos=this.state=0;e.elem=this.elem;if(e()&&c.timers.push(e)&&!ba)ba=setInterval(h.tick,h.interval)},show:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.show=true;this.custom(this.prop==="width"||this.prop==="height"?1:0,this.cur());c(this.elem).show()},hide:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.hide=true;

-

this.custom(this.cur(),0)},step:function(a){var b=c.now(),d=true;if(a||b>=this.options.duration+this.startTime){this.now=this.end;this.pos=this.state=1;this.update();this.options.curAnim[this.prop]=true;for(var e in this.options.curAnim)if(this.options.curAnim[e]!==true)d=false;if(d){if(this.options.overflow!=null&&!c.support.shrinkWrapBlocks){var f=this.elem,h=this.options;c.each(["","X","Y"],function(k,o){f.style["overflow"+o]=h.overflow[k]})}this.options.hide&&c(this.elem).hide();if(this.options.hide||

-

this.options.show)for(var l in this.options.curAnim)c.style(this.elem,l,this.options.orig[l]);this.options.complete.call(this.elem)}return false}else{a=b-this.startTime;this.state=a/this.options.duration;b=this.options.easing||(c.easing.swing?"swing":"linear");this.pos=c.easing[this.options.specialEasing&&this.options.specialEasing[this.prop]||b](this.state,a,0,1,this.options.duration);this.now=this.start+(this.end-this.start)*this.pos;this.update()}return true}};c.extend(c.fx,{tick:function(){for(var a=

-

c.timers,b=0;b<a.length;b++)a[b]()||a.splice(b--,1);a.length||c.fx.stop()},interval:13,stop:function(){clearInterval(ba);ba=null},speeds:{slow:600,fast:200,_default:400},step:{opacity:function(a){c.style(a.elem,"opacity",a.now)},_default:function(a){if(a.elem.style&&a.elem.style[a.prop]!=null)a.elem.style[a.prop]=(a.prop==="width"||a.prop==="height"?Math.max(0,a.now):a.now)+a.unit;else a.elem[a.prop]=a.now}}});if(c.expr&&c.expr.filters)c.expr.filters.animated=function(a){return c.grep(c.timers,function(b){return a===

-

b.elem}).length};var xb=/^t(?:able|d|h)$/i,Ia=/^(?:body|html)$/i;c.fn.offset="getBoundingClientRect"in t.documentElement?function(a){var b=this[0],d;if(a)return this.each(function(l){c.offset.setOffset(this,a,l)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);try{d=b.getBoundingClientRect()}catch(e){}var f=b.ownerDocument,h=f.documentElement;if(!d||!c.contains(h,b))return d||{top:0,left:0};b=f.body;f=fa(f);return{top:d.top+(f.pageYOffset||c.support.boxModel&&

-

h.scrollTop||b.scrollTop)-(h.clientTop||b.clientTop||0),left:d.left+(f.pageXOffset||c.support.boxModel&&h.scrollLeft||b.scrollLeft)-(h.clientLeft||b.clientLeft||0)}}:function(a){var b=this[0];if(a)return this.each(function(x){c.offset.setOffset(this,a,x)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);c.offset.initialize();var d,e=b.offsetParent,f=b.ownerDocument,h=f.documentElement,l=f.body;d=(f=f.defaultView)?f.getComputedStyle(b,null):b.currentStyle;

-

for(var k=b.offsetTop,o=b.offsetLeft;(b=b.parentNode)&&b!==l&&b!==h;){if(c.offset.supportsFixedPosition&&d.position==="fixed")break;d=f?f.getComputedStyle(b,null):b.currentStyle;k-=b.scrollTop;o-=b.scrollLeft;if(b===e){k+=b.offsetTop;o+=b.offsetLeft;if(c.offset.doesNotAddBorder&&!(c.offset.doesAddBorderForTableAndCells&&xb.test(b.nodeName))){k+=parseFloat(d.borderTopWidth)||0;o+=parseFloat(d.borderLeftWidth)||0}e=b.offsetParent}if(c.offset.subtractsBorderForOverflowNotVisible&&d.overflow!=="visible"){k+=

-

parseFloat(d.borderTopWidth)||0;o+=parseFloat(d.borderLeftWidth)||0}d=d}if(d.position==="relative"||d.position==="static"){k+=l.offsetTop;o+=l.offsetLeft}if(c.offset.supportsFixedPosition&&d.position==="fixed"){k+=Math.max(h.scrollTop,l.scrollTop);o+=Math.max(h.scrollLeft,l.scrollLeft)}return{top:k,left:o}};c.offset={initialize:function(){var a=t.body,b=t.createElement("div"),d,e,f,h=parseFloat(c.css(a,"marginTop"))||0;c.extend(b.style,{position:"absolute",top:0,left:0,margin:0,border:0,width:"1px",

-

height:"1px",visibility:"hidden"});b.innerHTML="<div style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;'><div></div></div><table style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;' cellpadding='0' cellspacing='0'><tr><td></td></tr></table>";a.insertBefore(b,a.firstChild);d=b.firstChild;e=d.firstChild;f=d.nextSibling.firstChild.firstChild;this.doesNotAddBorder=e.offsetTop!==5;this.doesAddBorderForTableAndCells=

-

f.offsetTop===5;e.style.position="fixed";e.style.top="20px";this.supportsFixedPosition=e.offsetTop===20||e.offsetTop===15;e.style.position=e.style.top="";d.style.overflow="hidden";d.style.position="relative";this.subtractsBorderForOverflowNotVisible=e.offsetTop===-5;this.doesNotIncludeMarginInBodyOffset=a.offsetTop!==h;a.removeChild(b);c.offset.initialize=c.noop},bodyOffset:function(a){var b=a.offsetTop,d=a.offsetLeft;c.offset.initialize();if(c.offset.doesNotIncludeMarginInBodyOffset){b+=parseFloat(c.css(a,

-

"marginTop"))||0;d+=parseFloat(c.css(a,"marginLeft"))||0}return{top:b,left:d}},setOffset:function(a,b,d){var e=c.css(a,"position");if(e==="static")a.style.position="relative";var f=c(a),h=f.offset(),l=c.css(a,"top"),k=c.css(a,"left"),o=e==="absolute"&&c.inArray("auto",[l,k])>-1;e={};var x={};if(o)x=f.position();l=o?x.top:parseInt(l,10)||0;k=o?x.left:parseInt(k,10)||0;if(c.isFunction(b))b=b.call(a,d,h);if(b.top!=null)e.top=b.top-h.top+l;if(b.left!=null)e.left=b.left-h.left+k;"using"in b?b.using.call(a,

-

e):f.css(e)}};c.fn.extend({position:function(){if(!this[0])return null;var a=this[0],b=this.offsetParent(),d=this.offset(),e=Ia.test(b[0].nodeName)?{top:0,left:0}:b.offset();d.top-=parseFloat(c.css(a,"marginTop"))||0;d.left-=parseFloat(c.css(a,"marginLeft"))||0;e.top+=parseFloat(c.css(b[0],"borderTopWidth"))||0;e.left+=parseFloat(c.css(b[0],"borderLeftWidth"))||0;return{top:d.top-e.top,left:d.left-e.left}},offsetParent:function(){return this.map(function(){for(var a=this.offsetParent||t.body;a&&!Ia.test(a.nodeName)&&

-

c.css(a,"position")==="static";)a=a.offsetParent;return a})}});c.each(["Left","Top"],function(a,b){var d="scroll"+b;c.fn[d]=function(e){var f=this[0],h;if(!f)return null;if(e!==B)return this.each(function(){if(h=fa(this))h.scrollTo(!a?e:c(h).scrollLeft(),a?e:c(h).scrollTop());else this[d]=e});else return(h=fa(f))?"pageXOffset"in h?h[a?"pageYOffset":"pageXOffset"]:c.support.boxModel&&h.document.documentElement[d]||h.document.body[d]:f[d]}});c.each(["Height","Width"],function(a,b){var d=b.toLowerCase();

-

c.fn["inner"+b]=function(){return this[0]?parseFloat(c.css(this[0],d,"padding")):null};c.fn["outer"+b]=function(e){return this[0]?parseFloat(c.css(this[0],d,e?"margin":"border")):null};c.fn[d]=function(e){var f=this[0];if(!f)return e==null?null:this;if(c.isFunction(e))return this.each(function(l){var k=c(this);k[d](e.call(this,l,k[d]()))});if(c.isWindow(f))return f.document.compatMode==="CSS1Compat"&&f.document.documentElement["client"+b]||f.document.body["client"+b];else if(f.nodeType===9)return Math.max(f.documentElement["client"+

-

b],f.body["scroll"+b],f.documentElement["scroll"+b],f.body["offset"+b],f.documentElement["offset"+b]);else if(e===B){f=c.css(f,d);var h=parseFloat(f);return c.isNaN(h)?f:h}else return this.css(d,typeof e==="string"?e:e+"px")}})})(window);

-

~~/* @Booklet~~

-

~~----------------------------------------*/~~

-

~~.booklet {width:800px; height:600px; position:relative; /*background:#fff;*/ margin:0 auto 10px;}~~

-

~~.booklet .b-page {left:0; top:0; position:absolute; overflow:hidden; padding:0;}~~

-

~~.booklet .b-pN {}~~

-

~~.booklet .b-p0 {}~~

-

~~.booklet .b-p1 {}~~

-

~~.booklet .b-p2 {}~~

-

~~.booklet .b-p3 {}~~

-

~~.booklet .b-p4 {}~~

-

~~/*.booklet .b-load {display:none;}*/~~

-

~~/* Page Wrappers */~~

-

~~.booklet .b-wrap {top:0; position:absolute;}~~

-

~~.booklet .b-wrap-left {background:#fff;}~~

-

~~.booklet .b-wrap-right {background:#efefef;}~~

-

~~.booklet .b-pN .b-wrap,~~

-

~~.booklet .b-p1 .b-wrap,~~

-

~~.booklet .b-p2 .b-wrap,~~

-

~~.booklet .b-p3 .b-wrap,~~

-

~~.booklet .b-p4 .b-wrap {left:0;}~~

-

~~.booklet .b-p0 .b-wrap {right:0;}~~

-

~~/* Custom Page Types */~~

-

~~.booklet .b-page-blank {padding:0; width:100%; height:100%;}~~

-

~~.booklet .b-page-cover {padding:0; width:100%; height:100%; background:#925C0E;}~~

-

~~.booklet .b-page-cover h3 {color:#fff; text-shadow:0px 1px 3px #222;}~~

-

~~/* Page Numbers */~~

-

.booklet .b-counter {bottom:10px; position:absolute; display:block; width:25px; height:20px; background:#ccc; color:#444; text-align:center; font-family:Georgia, "Times New Roman", Times, serif; font-size:10px; padding:5px 0 0;}

-

~~.booklet .b-wrap-left .b-counter {left:10px;}~~

-

~~.booklet .b-wrap-right .b-counter {right:10px;}~~

-

~~/* Page Shadows */~~

-

~~.booklet .b-shadow-f {right:0; top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-forward.png"); background-repeat:repeat-y; background-position:100% 0;}~~

-

~~.booklet .b-shadow-b {left:0; top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-back.png"); background-repeat:repeat-y; background-position:0 0;}~~

-

~~.booklet .b-p0 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:100% 10px;}~~

-

~~.booklet .b-p3 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:0 10px;}~~

-

~~/* Overlay Controls */~~

-

~~.booklet .b-overlay {top:0; position:absolute; height:100%; width:50%;}~~

-

~~.booklet .b-overlay-prev {left:0;}~~

-

~~.booklet .b-overlay-next {right:0;}~~

-

~~/* Tab Controls */~~

-

~~.booklet .b-tab {background:#000; height:40px; width:100%; padding:10px 0 0; text-align:center; color:#ccc; position:absolute; font-size:11px;}~~

-

~~.booklet .b-tab-prev {left:0;}~~

-

~~.booklet .b-tab-next {right:0;}~~

-

~~.booklet .b-tab:hover {background:#aaa; color:#000;}~~

-

~~/* Arrow Controls */~~

-

~~.booklet .b-arrow {display:block; position:absolute; text-indent:-9999px; top:0; height:100%; width:74px;}~~

-

~~.booklet .b-arrow div {display:none; text-indent:-9999px; height:170px; width:74px; position:absolute; left:0; top:25%;}~~

-

~~.booklet .b-arrow-next {right:-80px;}~~

-

~~.booklet .b-arrow-prev {left:-80px;}~~

-

~~.booklet .b-arrow-next div {background:url("images/arrow-next.png") no-repeat left top;}~~

-

~~.booklet .b-arrow-prev div {background:url("images/arrow-prev.png") no-repeat left top;}~~

-

~~/* @z-index fix (needed for older IE browsers)~~

-

~~----------------------------------------*/~~

-

~~body {z-index:0;}~~

-

~~.b-menu {z-index:100;}~~

-

~~.b-selector {z-index:100;}~~

-

~~.booklet {z-index:10;}~~

-

~~.b-pN {z-index:10;}~~

-

~~.b-p0 {z-index:10;}~~

-

~~.b-p1 {z-index:10;}~~

-

~~.b-p2 {z-index:10;}~~

-

~~.b-p3 {z-index:10;}~~

-

~~.b-p4 {z-index:10;}~~

-

~~.b-prev {z-index:40;}~~

-

~~.b-next {z-index:40;}~~

-

~~.b-counter {z-index:40;}~~

-

~~/* @Menu Items~~

-

~~----------------------------------------*/~~

-

~~.b-menu {height:40px; padding:0 0 10px;}~~

-

~~.b-selector {height:40px; position:relative; float:right; border:none; color:#cecece; cursor:pointer;}~~

-

~~.b-selector .b-current {padding:8px 15px 12px; line-height:20px; min-width:18px; height:20px; display:block; background:#000; text-align:center;}~~

-

~~.b-selector-page {width:auto; margin-left:15px;}~~

-

~~.b-selector-chapter {width:auto;}~~

-

~~.b-selector:hover {color:#fff; background-position:left 0px;}~~

-

~~.b-selector:hover .b-current {background-position:right 0px;}~~

-

.b-selector ul {overflow:hidden; margin:0; list-style:none !important; position:absolute; top:40px; right:0; padding:0 0 10px; background:#000; width:240px; font:normal 12px "Myriad Pro", Myriad, "DejaVu Sans Condensed","Liberation Sans", "Nimbus Sans L", Tahoma, Geneva, "Helvetica Neue", Helvetica, Arial, sans-serif;}

-

~~.b-selector li {border:none;}~~

-

~~.b-selector a {color:#cecece; height:14px; text-decoration:none; display:block; padding:5px 10px;}~~

-

~~.b-selector a .b-text {float:left; clear:none;}~~

-

~~.b-selector a .b-num {float:right; clear:none;}~~

-

~~.b-selector a:hover {color:#fff;}~~

-

~~$(function() {~~

-

~~var $mybook = $('#mybook');~~

-

~~var $bttn_next = $('#next_page_button');~~

-

~~var $bttn_prev = $('#prev_page_button');~~

-

~~var $loading = $('#loading');~~

-

~~var $mybook_images = $mybook.find('img');~~

-

~~var cnt_images = $mybook_images.length;~~

-

~~var loaded = 0;~~

-

~~//preload all the images in the book,~~

-

~~//and then call the booklet plugin~~

-

~~$mybook_images.each(function(){~~

-

~~var $img = $(this);~~

-

~~var source = $img.attr('src');~~

-

~~$('<img/>').load(function(){~~

-

~~++loaded;~~

-

~~if(loaded == cnt_images){~~

-

~~$loading.hide();~~

-

~~$bttn_next.show();~~

-

~~$bttn_prev.show();~~

-

~~$mybook.show().booklet({~~

-

~~name: null, // name of the booklet to display in the document title bar~~

-

~~width: 800, // container width~~

-

~~height: 500, // container height~~

-

~~speed: 600, // speed of the transition between pages~~

-

~~direction: 'LTR', // direction of the overall content organization, default LTR, left to right, can be RTL for languages which read right to left~~

-

~~startingPage: 0, // index of the first page to be displayed~~

-

~~easing: 'easeInOutQuad', // easing method for complete transition~~

-

~~easeIn: 'easeInQuad', // easing method for first half of transition~~

-

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~~<body>~~

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~~<h1 class="title"> Protocols //Notebook </h1>~~

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~~<div class="book_wrapper"> <a id="next_page_button"></a> <a id="prev_page_button"></a>~~

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~~<div class="b-load">~~

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~~<div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/8/88/FotoiGEM1.jpg" />~~

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~~<h1>Protocol 1 </h1>~~

-

~~<p>Preparation of culture media (1L).<br />~~

-

~~1.- 5 g of yeast<br />~~

-

~~2.- 10 g of sodium chloride.<br />~~

-

~~3.- 10 g of triptone.<br />~~

-

~~4.- Add water till 1 uL volume.</p>~~

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~~</div>~~

-

~~<div>~~

-

~~<h1>Protocol 2</h1>~~

-

~~<p>Competent cell´s preparation<br />~~

-

~~<br />~~

-

~~1.- Centrifug your media culture tube (25 mL) at 4,000 rpm during~~

-

~~15 min.<br />~~

-

~~2.- Pour the supernatant and add 10 mL of cold MgCl2 solution.~~

-

~~Centrifuge them at 4,000 rpm at 4 °C during 15 min.<br />~~

-

~~3.- Pour the supernatant and resuspend the pellet in 40 mL of cold~~

-

~~CaCl2 solution. Keep it in ice during 20 min and place them in~~

-

~~eppendorf tubes. Centrifuge them at 4,000 rpm at 4°C during 15~~

-

~~min.<br />~~

-

~~4.- Pour the supernatant and resuspend the pellet in 40 mL of cold~~

-

~~85mM CaCl2/15% glycerlol v/v solution. Centrifuge again at 2,100~~

-

~~rpm at 4°C during 15 min.<br />~~

-

~~5.- Pour the supernatant and resuspend in 2 mL of cold 85bmM~~

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~~CaCl2/15% glycerol v/v solution.<br />~~

-

~~6.- Aliquote in tubes of 100 uL previously frozen and store at~~

-

~~-80°C.<br />~~

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~~</p>~~

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~~</div>~~

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~~<div>~~

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~~<h1>Protocol 3</h1>~~

-

~~<p>DNA extraction from Buffy coat:<br />~~

-

~~<br />~~

-

~~<font size="1"> 1.- Centrifuge 5 mL of culture medium at 1,500 rpm~~

-

~~for 10 min at 4°C.</font><br />~~

-

~~<font size="1">2.- Carefully transfer 600 uL of buffy coat to a~~

-

~~microtube.</font><br />~~

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~~<font size="1">3.- Add 1 mL of RBC buffer and mix throughly by~~

-

~~vortex.</font><br />~~

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~~<font size="1">4.- Centrifuge at 4,500 rpm during 30 s and pour~~

-

~~carefully so your pellet is not wasted.</font><br />~~

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~~<font size="1">5.- Repeat steps 2 and 3 adding just 500 uL from~~

-

~~RBC buffer.</font><br />~~

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~~<font size="1">6.- Once separated, add 300 uL of lysis buffer to~~

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~~the pellet and mix by vortex.</font><br />~~

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~~<font size="1">7.- Add 150 uL of protein precipitation solution.</font><br />~~

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~~<font size="1">8.- Centrifuge at 11,000 rpm during 5 min; then,~~

-

~~transfer the supernatant to a new microtube.</font><br />~~

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~~<font size="1">9.- Add 700 uL of cold isopropanol and mix by~~

-

~~inverting until you see a white formation within the tube.</font><br />~~

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~~<font size="1">10.- Centrifuge at 11,000 rpm during 4 min and pour~~

-

~~carefully taking care of the pellet at the bottom.</font><br />~~

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~~<font size="1">11.- Add 500 uL of cold ethanol 70% to wash and~~

-

~~then centrifuge at 11,000 rpm during 4 min.</font><br />~~

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~~<font size="1">12.- Pour and let the ethanol evaporate at room~~

-

~~temperature.</font><br />~~

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~~<font size="1"> 13.- Resuspend the DNA pellet with 12 uL of TE~~

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~~buffer; incubate all night at 4 °C.</font><br />~~

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~~</p>~~

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~~</div>~~

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~~<div>~~

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~~<h1>Protocol 4</h1>~~

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~~<p>Electrophoresis:<br />~~

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~~<br />~~

-

~~<font size="1">1.- Prepare 1L of TAE 10X (48.4g of Tris base[ tris~~

-

~~(hidroxymethyl) amminomethane], 11.4 mL of glacial acetic acid~~

-

~~(17.4 M) and 3.7 g of EDTA then fill with deionized water to 1~~

-

~~L.</font><br />~~

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~~<font size="1">2.- Prepare a 50 mL solution of 3% agarose using~~

-

~~TAE buffer (without using water).</font><br />~~

-

~~<font size="1">3.- Heat the gel in the microwave in intervals of~~

-

~~30 s, 20 s and 10 s until the agarose is completely dissolved,~~

-

~~taking care that it does not boil so much.</font><br />~~

-

~~<font size="1">4.- Let cool down the gel (55-60°C) and pour in a~~

-

~~base with the comb already on its place to form the charging~~

-

~~wells.</font><br />~~

-

~~<font size="1">5.- Wait until the gel solidifies.</font><br />~~

-

~~<font size="1">6.- Remove the comb and place the base with the gel~~

-

~~inside the electrophoretic chamber.</font><br />~~

-

~~<font size="1">7.- Add TAE buffer until the gel is covered.</font><br />~~

-

~~<font size="1">8.- Take 5 uL of each sample from the PCR and 1 uL~~

-

~~of charging gel in a new PCR tube, mix well.</font><br />~~

-

~~<font size="1">9.- Charge the samples inside the wells of the gel,~~

-

~~adding a weight marcker.</font><br />~~

-

~~<font size="1">10.- Close the electrophoretic chamber and run at~~

-

~~120 V during 30 min. Follow the displacement ofthe samples.</font><br />~~

-

~~<font size="1">11.- Take out the gel from the chamber very~~

-

~~carefully and place in the UV light to see the DNA strands.</font><br />~~

-

~~</p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1>Protocol 5</h1>~~

-

~~<p>Competent cell transformation:<br />~~

-

~~<br />~~

-

~~1.- Place 1 uL of the plasmid in a microtube, gently add 100 uL of~~

-

~~your competent cells. Take another tube and place only 100 uL of~~

-

~~competent cells, so you have a control group.<br />~~

-

~~2.- Mix gently with a pipette and incubate during 30 min in ice.<br />~~

-

~~3.- Ab ruptly place your cells in 42°C dry bath during 2 min and~~

-

~~replace in ice whwn finished.<br />~~

-

~~4.- Add 900 uL of LB medium and incubate at 37 °C for 30 min.<br />~~

-

~~5.- Inoculate with 200 uL of your transformed cells in LB-Amp.<br />~~

-

~~6.- Incubate overnight and store at 4 °C of freeze in 15-25%~~

-

~~glycerol.<br />~~

-

~~</p>~~

-

~~</div>~~

-

~~<div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/e/e4/LabArchives.jpg" />~~

-

~~<h1>Lab archives// Mar 12, 2014 </h1>~~

-

~~<p>In order to grow our bacteria, we inoculated with 100 uL of E.~~

-

~~Coli DH5-alpha in 50 mL of LB medium, and 100 uL of E. Coli TOP in~~

-

~~50 mL of LB medium. We left both samples incubating overnight </p>~~

-

~~<h1>Lab archives// Mar 20, 2014 </h1>~~

-

~~<p>We inoculated E.Coli NEB10-beta in 50 ml of LB medium. </p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1>Lab archives// Mar 27, 2014 </h1>~~

-

~~<p>We prepared the medium in which our bacteria will grow, for that~~

-

~~we dissolved solid LB with agarose, then we add it into 10 plates,~~

-

~~we waited a few minutes for it to solidify and finally we added~~

-

~~500 uL of our antibiotic that is chloranphenicol to each of those~~

-

~~plates. </p>~~

-

~~<h1>Lab archives// Apr 01, 2014 </h1>~~

-

~~<p>we transformed luciferaze from DNA to cells by incerting them~~

-

~~with change of temperature </p>~~

-

~~<h1>Lab archives// Apr 02, 2014 </h1>~~

-

~~<p>We made a litter of agar and 500 L of growth medium, and we~~

-

~~sterilized them along with crystal media disperssion balls </p>~~

-

~~<h1>Lab archives// Apr 03, 2014 </h1>~~

-

~~<p>Today we prepared buffers for home made mini preps and did a cell~~

-

~~re culture. </p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1">Lab archives// Apr 07, 2014 </font> </h1>~~

-

~~<p> <font size="1">We added 700 mL of water in a beaker and then we~~

-

~~prepared 23 grams of powdered agar that we added slowly while we~~

-

~~moved the beaker. This in order to help the dissolve the powder.~~

-

~~After that, we added other 300 mL to the solution and finally we~~

-

~~passed it to an Erlenmeyer matrass. Also we prepared 500 mL of~~

-

~~growth medium. First, we measured and added 5 grams of tryptone,~~

-

~~5 grams of yeast and 10 grams of sodium chloride. Those~~

-

~~ingredients were added slowly to a graduated cylinder with 350~~

-

~~mL of wáter while the flask was moved. After finishing the~~

-

~~addition we added other 150 mL of water. The solution was then~~

-

~~transfered to another Erlenmeyer flask. Both Erlenmeyer flasks~~

-

~~and a glass bottle with crystal media diperssion balls were~~

-

~~sterilized with temperature and pressure.</font> </p>~~

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~~<h1><font size="1">Lab archives// Apr 02, 2014 </font> </h1>~~

-

~~<p><font size="1">300 ul of ampicillin at 100mg/ml chloramphenicol </font><br />~~

-

~~<font size="1"> 3 Plates with agar </font><br />~~

-

~~<font size="1">Promoters: BBa_K258005 (pO2), BBa_176500 (pFe)~~

-

~~BBa_J52008 (luc-3) </font> <br />~~

-

~~<font size="1">The plates were impregnated with ampicillin~~

-

~~promoting bacteria in the following manner </font><br />~~

-

-

~~<font size="1">~~

-

~~------------------------------------------------------ </font><br />~~

-

-

~~<font size="1">~~

-

~~------------------------------------------------------ </font><br />~~

-

-

~~</p>~~

-

~~<p></p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1">Apr 08, 2014 </font></h1>~~

-

~~<p> <font size="1"> **/Today we realized the mini prep for the~~

-

~~oxygen promoter. /** </font><br />~~

-

~~<font size="1"> --500 ul of Ampiciline //this is for the oxygen~~

-

~~promoter </font><br />~~

-

~~<font size="1"> --50 ul of oxygen promoter </font><br />~~

-

~~<font size="1"> --45 ml of LB medium </font><br />~~

-

~~<font size="1"> --at room temperature 15 minutes at 1500 rpm in~~

-

~~the centrifuge. </font><br />~~

-

~~<font size="1"> --500 ul of chloramphenicol // this is for the red~~

-

~~liciferase </font><br />~~

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~~<font size="1"> --50 ul of red luciferase </font><br />~~

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~~<font size="1"> --45 ml of LB medium </font><br />~~

-

~~<font size="1"> --The new LB medium was prepared with //LB medium~~

-

~~</font><br />~~

-

~~<font size="1"> --10 g yeast </font><br />~~

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~~<font size="1"> --5 g salt </font><br />~~

-

~~<font size="1"> --15 g Tryptone </font> <font size="1"> MINI~~

-

~~PREP First 400 ul of Genomic Lysis Buffer to the 40 ml overnight~~

-

~~[bacteriaand oxygen promoter] Then the mixture is transferred to~~

-

~~a Zyne-Spine Collumn in a Collection Tube. Centrifuge 10,000 rpm~~

-

~~/1 min Transfer the Zyno Spin Column to a new Collection Tube.~~

-

~~Add 200 micro liters of Pre Wash Buffer. Centrifuge 10,000 rpm~~

-

~~/1 min Add 500 uL of of g-DNA Wash buffer to the spin column.~~

-

~~Centrifuge 10,000 rpm /1 min Transfer the spin column to a clear~~

-

~~micro centrifuge tube. Add 50 uL of DNA Elution Buffer .~~

-

~~Incubate 2-5 minutes to at room temperature. Centrifuge at top~~

-

~~speed per 30 seconds. Then store at -80 °C </font></p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1"> Apr 22, 2014</font> </h1>~~

-

~~<p><font size="1"> We prepared 2 overnight cultures (45ml of LB~~

-

~~medium): GFP E0040 pSB1A2 with 100 uL of ampicillin. M-cherry~~

-

~~(RFP) BBa_J04450 pSB1C3 with 500 uL of chloramphenicol.</font></p>~~

-

~~<h1><font size="1"> Apr 24, 2014 </font></h1>~~

-

~~<p><font size="1"> 2 Transformations: Bacteria NEB 10 Beta, DH5alpha~~

-

~~2 Mini Preps: GFP, RFP //Transformation and preps:--~~

-

~~Centrifugation at 2500 rpm for 15 min.--1ul DNA in 100 uL of~~

-

~~bacterial culture (luciferase). --Filtering GFP, RFP bacteria.~~

-

~~--Added 400 ml of Lysis Buffer Genomyc each crop by columns.~~

-

~~--Centrifugation at 10,000 * g for 1 min. --Last column to~~

-

~~another tube and DNA Prewash + 200 uL Buffer. --Centrifuged at~~

-

~~10,000 * g for 1 min. --Add 500ul of DNA Wash Buffer,~~

-

~~centrifuged at 10,000 * g for 1 min. --Transfer to clean tube,~~

-

~~add 50ul of DNA Elution Buffer. --Incubate for 2-5 min at room~~

-

~~temperature, centrifuged at maximum speed for 30 sec.</font></p>~~

-

~~<h1><font size="1"> Apr 28, 2014 </font></h1>~~

-

~~<p><font size="1"> Today was prepared LB media. It also was done the~~

-

~~sterilization of 1 mL and 200 uL tips, water and ependorfs.~~

-

~~After the sterilization of the LB media was done, we cultivated~~

-

~~in it the bacteria carrying the oxygen promoter, RFP, and GFP.~~

-

~~Then we left the bacteria growing in the incubator at 37°C~~

-

~~overnight.</font></p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1"> May 05, 2014 </font></h1>~~

-

~~<p><font size="1"> Plating bacteria with GFP, RFP, promoter Oxygen~~

-

~~and iron promoter plasmids. Everyone in their respective~~

-

~~environments and specific antibiotics (ampicillin and~~

-

~~chloramphenicol). Tubes with bacteria was added and placed them~~

-

~~in LB medium incubator to grow more product if necessary.</font></p>~~

-

~~<h1><font size="1"> May 08, 2014 </font></h1>~~

-

~~<p><font size="1"> We did a centrifuge at 4800 rpm for 20 min for~~

-

~~the plasmids had been in overnight which had oxygen promoter,~~

-

~~GFP and RFP to rush after we separate the aqueous phase.</font></p>~~

-

~~<h1><font size="1"> May 19, 2014 </font></h1>~~

-

~~<p><font size="1"> We did inoculation of GFP, RFP and Oxigen Prmoter</font></p>~~

-

~~<h1><font size="1"> May 20, 2014 </font></h1>~~

-

~~<p><font size="1"> We started minipreps preparation of GFP, RFP and~~

-

~~Oxigen Promoter. We added 1.5 ml of culture to each Eppendorff~~

-

~~(4 for each promoter) and we centrifugated it at 13.2 rpm for 10~~

-

~~minutes, we did the same procedure 2 times. Also, we sterilized~~

-

~~two Erlenmeyer flasks and 40 Eppendorffs. </font></p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1"> May 20, 2014 //Mini prep preparation </font></h1>~~

-

~~<p><font size="1"> 1. We added 1.5 mL of the cultures to their~~

-

~~corresponding Eppendorffs and centrifugated them at 3400 rpm~~

-

~~during 10 minutes other three times.</font></p>~~

-

~~<p><font size="1">2. Once we had a pellet we discarded the~~

-

~~supernatant and we added 300 uL of TEG buffer (made of 100 mM of~~

-

~~Tris-HCl pH8, 2 mM EDTA and 20% glucose)</font></p>~~

-

~~<p><font size="1">3. We added 700 μL of NS solution (0.2 M NaOH, 1%~~

-

~~w/v SDs) and we mixed by inversion.</font></p>~~

-

~~<p><font size="1">4. We added 400 μL of 3 M sodium acetate pH 5.3,~~

-

~~and we mixed by inversion.</font></p>~~

-

~~<p><font size="1">5. We centrifugated the Eppendorffs at 3400 rpm~~

-

~~during 15 minutes.</font></p>~~

-

~~<p><font size="1">6. We transfered the supernatant to other~~

-

~~Eppendorffs and we added 50 uL of cold isopropanol and mixed by~~

-

~~inversion.</font></p>~~

-

~~<p><font size="1">7. We centrifugated the Eppendorffs at 3400 rpm~~

-

~~during 15 minutes.</font></p>~~

-

~~<p><font size="1">8. We discarded the supernatant and washed each~~

-

~~pellet with 70% ethanol and we let it dry for 15 minutes.</font></p>~~

-

~~<p><font size="1">9. We resuspended the pellet in 500 uL of TE~~

-

~~buffer and then we added 10 mg/mL RNAseA.</font></p>~~

-

~~<p><font size="1">11. We precipitated the DNA with 100 uL of ethanol~~

-

~~in each Eppendorff.</font></p>~~

-

~~<p><font size="1">12. We centrifugated for 1 minute at 3400 rpm.</font></p>~~

-

~~<p><font size="1">13. We got rid of the supernatant.</font></p>~~

-

~~<p><font size="1">14. Finally, we resuspended the pellet in 50 uL of~~

-

~~TE buffer</font></p>~~

-

~~</div>~~

-

~~<div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/f/f4/1%281%29.jpg" />~~

-

~~<h1>UNAM procedures// Digestion</h1>~~

-

~~<p>|BSA | 1 uL | </p>~~

-

~~<p>|DNA | 2 uL | </p>~~

-

~~<p>|Buffer (2) | 1 uL |</p>~~

-

~~<p>|Buffer Xba I+Pstl | 3.3 uL |</p>~~

-

~~<p>|fe Xba I+Spef | 3.3 uL | </p>~~

-

~~<p>|H<sub>2</sub>0 | 5.4 uL | </p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1><font size="1">Gel agarose</font></h1>~~

-

~~<p><font size="1"> 1 kb NEB Ampyciline -> Pentneryl </font></p>~~

-

~~<p><font size="1">| ---------------Bristol - Myers Squibb</font> </p>~~

-

~~<p><font size="1">| ---------------1 gram/ 3 mL</font> </p>~~

-

~~<p><font size="1">| ---------------inyectable</font></p>~~

-

~~<p><font size="1">|<sub>p</sub>O <sub>2</sub> | 3.3 uL</font> </p>~~

-

~~<p><font size="1">|1 kb marker 5.4 uL </font></p>~~

-

~~<h1><font size="1">Homemade Mini prep Preparation </font></h1>~~

-

~~<p><font size="1"> 1.- Overnight bacterial culture of 5 mL in~~

-

~~falkons of 50 mL </font></p>~~

-

~~<p><font size="1"> 2.- Centrifugate in an eppendor of 1.5 mL and~~

-

~~throw supernadant</font></p>~~

-

~~<p><font size="1"> 3.- Add 152 uL of Buffer 1 + RNAse (11~~

-

~~micro-liters /1 buffer micro-liter ) **Buffer 20% Glucouse </font></p>~~

-

~~<p><font size="1"> 4.- Mix via Buffer and add 150 uL of Buffer 2~~

-

*Wait 4-5 minutes*</font> </p>

-

~~<p><font size="1"> (1.5mL)Buffer 2 -> 880 uL H<sub>2</sub>O,20uL~~

-

~~NaOH 10 N </font></p>~~

-

~~<p><font size="1"> ---------------------------------------100 uL SDS~~

-

~~10%</font> </p>~~

-

~~<p><font size="1"> 5.- Mix via inversion and add150 uL Buffer 3 </font>~~

-

~~</p>~~

-

~~<p><font size="1"> ------------------------------Sodium Acetate 3M~~

-

~~pH 5</font> </p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<p><font size="1"> 6.-Centrifuge 5 minutes.</font> </p>~~

-

~~<p><font size="1"> 7.-Take the supernadant to antoher tube. Add 1 mL~~

-

~~ETOH Absolute </font></p>~~

-

~~<p><font size="1"> 8.-Centrifuge by 5 minutes and decant </font> </p>~~

-

~~<p><font size="1"> 9.-Wash with 500uL ETOH 70 %</font> </p>~~

-

~~<p><font size="1"> 10.-Centrifugate 1 minute </font></p>~~

-

~~<p><font size="1"> 11.-Decant and dry at 37 celsius centigrades (30~~

-

~~minutes)</font> </p>~~

-

~~<p><font size="1"> 12.-Resuspend in 15 uL of H<sub>2</sub>O </font></p>~~

-

~~<h1><font size="1">Ligation</font></h1>~~

-

~~<p><font size="1"> Inserto 7mL</font></p>~~

-

~~<p><font size="1"> Plasmid 3mL</font> </p>~~

-

~~<p><font size="1"> Buffer 10x 1.5mL </font> </p>~~

-

~~<p><font size="1"> Ligase 0.5 mL </font></p>~~

-

~~<p><font size="1"> H<sub>2</sub>O 3 mL </font> </p>~~

-

~~<p><font size="1"> Total = 15 ml </font></p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1>E.Coli cells transformation. </h1>~~

-

~~<p>To transform E.Coli cells with an exogen DNA, 200 uL of competent~~

-

~~cells were taken they were mixed with 1-20 ug(Micrograms) of DNA~~

-

~~that was incubated on ice for 30 minutes. This makes posible the~~

-

~~absorbtion of the exogen DNA to the cells surface, after that, the~~

-

~~cells are incubated at 42Celsius Degrees during 90 seconds, for~~

-

~~then incubate them during 1 minute again in ice. THis termal shock~~

-

~~makes posible the entrance of the plasmid to the inside of the~~

-

~~cell. 600 uL of LB medium were added and then incubated during~~

-

~~60-90 minutes at 37 Celisus Degres, to do posible the segregation~~

-

~~of the exogenic plasmid during the celular division (3 to 5~~

-

~~generations). Finally, Alicuote of 100-200 uL were cultivated by~~

-

~~dimention in the LB agar medium, which ere incubated at 37 Celsius~~

-

~~Degrees. After 15-20 hours of incuvation colonies could be~~

-

~~observed</p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<h1>Ligations</h1>~~

-

~~<p>1# Grow h mL in overnight</p>~~

-

~~<p>2# Do minipreps</p>~~

-

~~<p>3# EcoRI and PstI restrictions.</p>~~

-

~~<p> --for Fe EcoRIand SpeI</p>~~

-

~~<p>O<sub>2</sub>+ GFP ---1,2,3 - 931</p>~~

-

~~<p>Fe+GTP ---4,5,6 -1,828</p>~~

-

~~<p>O<sub>2</sub>+ RFP ---7,8,9,10,11,12,13 -890</p>~~

-

~~<p>Fe+RFP ---14,15,16,17,18,19 -1,787</p>~~

-

~~<br />~~

-

~~<p>GFP -> 761 bp EcoRI Po+I // 728 bp XbaI SpeI</p>~~

-

~~<p>RFP -> 714 bp Xba - SpeI // 747 bp EcoR - Ps+I</p>~~

-

~~<br />~~

-

~~<p>pFe -> 1,067 bp EcoRI - SpeI </p>~~

-

~~<p><sub>p</sub>O<sub>2</sub> -> 178 bp EcoRi SpeI // 145 bp Xba -~~

-

~~Ps+I </p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<p>O<sub>2</sub> + GFP 1/1 - 1/2 - 3/3</p>~~

-

~~<p>FO <sub>2</sub> + GFP 4/1 - 5/2 - 6/3</p>~~

-

~~<p>O<sub>2</sub> + RFP 7/1 - 8/2 - 9/3 - 10/4 - 11/5 - 12/6 - 12/13~~

-

~~</p>~~

-

~~<p>Fe + RFP 13/1 - 14/2 - 15*16/3 - 17/4 - 18/5 -19/6</p>~~

-

~~<h1>Digestion</h1>~~

-

~~<p>2 --- BSA --- 1uL</p>~~

-

~~<p>4 --- DNA --- 2uL</p>~~

-

~~<p>3 --- (M) Buffer // Enz (0.3 uL each one)--- 1uL</p>~~

-

~~<p>5 --- EcoRI --- 0.3 uL</p>~~

-

~~<p> --- PstI --- 0.3 uL</p>~~

-

~~<p>1 --- H<sub>2</sub>O --- 5.4 uL</p>~~

-

~~<p> Total == 10 uL rxn</p>~~

-

~~<br />~~

-

~~<p> GFP 761 bp</p>~~

-

~~<p> RFP 742 bp</p>~~

-

~~</div>~~

-

~~<div>~~

-

~~<p> pFe 1,067 bp </p>~~

-

~~<p> pO<sub>2</sub> -15.5 bp</p>~~

-

~~<br />~~

-

~~<p> pO<sub>2</sub> + GFP 931 bp</p>~~

-

~~<p> pFe + GFP 1,828 bp</p>~~

-

~~<p> pO<sub>2</sub>+ RFP 890 bp</p>~~

-

~~<p> pFe + RFP 1,787 bp</p>~~

-

~~<br />~~

-

~~<p> Gel 1 = 8/9/10/Lodder 1kb/11/12/13</p>~~

-

~~<p> Gel 2 = 15/16/17/Lodder 116/18/19</p>~~

-

~~<p> Gel 3 = 1/2/3/4/Lodder 1kb/5/6/7</p>~~

-

~~</div>~~

-

~~</div>~~

-

~~</div>~~

-

~~</div>~~

-

~~<div> <span class="reference"> </span> </div>~~

-

~~</body>~~

-

~~</html>~~

@@ Line 1: / Line 1: @@
-<html>
-<head>
-<style>
-*{
-	margin:0;
-	padding:0;
-}
-body{
-	background:#ccc url(../images/DNA.jpg) repeat top left;
-	font-family: Arial, Helvetica, sans-serif;
-	color:#444;
-	font-size:12px;
-	color: #000;
-}
-h1{
-	color:#000000;
-	font-size:40px;
-	margin:20px 0px 0px 20px;
-}
-span.reference{
-	font-family:Arial;
-	display:block;
-	font-size:12px;
-	text-align:center;
-	margin-bottom:10px;
-}
-span.reference a{
-	color:#000;
-	text-transform:uppercase;
-	text-decoration:none;
-	margin:0px 20px;
-}
-span.reference a:hover{
-	color:#ddd;
-}
-/* Booklet jQuery Plugin Style*/
-.booklet{
-	-moz-box-shadow:0px 0px 1px #fff;
-	-webkit-box-shadow:0px 0px 1px #fff;
-	box-shadow:0px 0px 1px #fff;
-	-moz-border-radius:10px;
-	-webkit-border-radius:10px;
-	border-radius:10px;
-}
-.booklet .b-wrap-left  {
-	background:#fff url(../images/left_bg.jpg) no-repeat top left;
-	-webkit-border-top-left-radius: 10px;
-	-webkit-border-bottom-left-radius: 10px;
-	-moz-border-radius-topleft:10px;
-	-moz-border-radius-bottomleft: 10px;
-	border-top-left-radius: 10px;
-	border-bottom-left-radius: 10px;
-}
-.booklet .b-wrap-right {
-	background:#efefef url(../images/right_bg.jpg) no-repeat top left;
-	-webkit-border-top-right-radius: 10px;
-	-webkit-border-bottom-right-radius: 10px;
-	-moz-border-radius-topright: 10px;
-	-moz-border-radius-bottomright: 10px;
-	border-top-right-radius: 10px;
-	border-bottom-right-radius: 10px;
-}
-.booklet .b-counter {
-	bottom:10px;
-	position:absolute;
-	display:block;
-	width:90%;
-	height:20px;
-	border-top:1px solid #ddd;
-	color:#222;
-	text-align:center;
-	font-size:12px;
-	padding:5px 0 0;
-	background:transparent;
-	-moz-box-shadow:0px -1px 1px #fff;
-	-webkit-box-shadow:0px -1px 1px #fff;
-	box-shadow:0px -1px 1px #fff;
-	opacity:0.8;
-}
-.book_wrapper{
-	margin:0 auto;
-	padding-top:50px;
-	width:905px;
-	height:540px;
-	position:relative;
-	background:transparent url(../images/bg.png) no-repeat 9px 27px;
-}
-.book_wrapper h1{
-	color:#13386a;
-	margin:5px 5px 5px 15px;
-	font-size:26px;
-	background:transparent url(../images/h1.png) no-repeat bottom left;
-	padding-bottom:7px;
-}
-.book_wrapper p{
-	font-size:16px;
-	margin:5px 5px 5px 15px;
-}
-.book_wrapper a.article,
-.book_wrapper a.demo{
-	background:transparent url(../images/circle.png) no-repeat 50% 0px;
-	display:block;
-	width:95px;
-	height:41px;
-	text-decoration:none;
-	outline:none;
-	font-size:16px;
-	color:#555;
-	float:left;
-	line-height:41px;
-	padding-left:47px;
-}
-.book_wrapper a.demo{
-	margin-left:50px;
-}
-.book_wrapper a.article:hover,
-.book_wrapper a.demo:hover{
-	background-position:50% -41px;
-	color:#13386a;
-}
-.book_wrapper img{
-	margin:10px 0px 5px 35px;
-	width:300px;
-	padding:4px;
-	border:1px solid #ddd;
-	-moz-box-shadow:1px 1px 1px #fff;
-	-webkit-box-shadow:1px 1px 1px #fff;
-	box-shadow:1px 1px 1px #fff;
-}
-.booklet .b-wrap-right img{
-	border:1px solid #E6E3C2;
-}
-a#next_page_button,
-a#prev_page_button{
-	display:none;
-	position:absolute;
-	width:41px;
-	height:40px;
-	cursor:pointer;
-	margin-top:-20px;
-	top:50%;
-	background:transparent url(../images/buttons.png) no-repeat 0px -40px;
-}
-a#prev_page_button{
-	left:-30px;
-}
-a#next_page_button{
-	right:-30px;
-	background-position:-41px -40px;
-}
-a#next_page_button:hover{
-	background-position:-41px 0px;
-}
-a#prev_page_button:hover{
-	background-position:0px 0px;
-}
-.loading{
-	width:160px;
-	height:56px;
-	position: absolute;
-	top:50%;
-	margin-top:-28px;
-	right:135px;
-	line-height:56px;
-	color:#fff;
-	padding-left:60px;
-	font-size:15px;
-	background: #000 url(../images/ajax-loader.gif) no-repeat 10px 50%;
-	opacity: 0.7;
-	z-index:9999;
-	-moz-border-radius:20px;
-	-webkit-border-radius:20px;
-	border-radius:20px;
-	filter:progid:DXImageTransform.Microsoft.Alpha(opacity=70);
-}
-</style>
-<script>
-/*
- * jQuery Booklet Plugin
- * Copyright (c) 2010 W. Grauvogel (http://builtbywill.com/)
- *
- * Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)
- * and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.
- *
- * Version : 1.1.0
- *
- * Originally based on the work of:
- *	1) Charles Mangin (http://clickheredammit.com/pageflip/)
- */
-;(function($) {
-$.fn.booklet = function(options){
-	var o = $.extend({}, $.fn.booklet.defaults, options);
-	return $(this).each(function()
-	{
-		var command, config, obj, id, i, target;
-		//option type string - api call
-		if(typeof options == 'string')
-		{
-			//check if booklet has been initialized
-			 if($(this).data('booklet')){
-				command = options.toLowerCase();
-				obj = $.fn.booklet.interfaces[$(this).data('id')];
-				if(command == 'next'){ obj.next() }
-				else if(command == 'prev'){ obj.prev() }
-			 }
-		}
-		//option type number - api call
-		else if(typeof options == 'number')
-		{
-			//check if booklet has been initialized
-			 if($(this).data('booklet')){
-				target = options;
-				obj = $.fn.booklet.interfaces[$(this).data('id')];
-				if(target % 2 != 0) {
-					target-= 1;
-				}
-				obj.gotoPage(target);
-			 }
-		}
-		//else build new booklet
-		else
-		{
-			config = $.extend(true, {}, o);
-			// Determine ID (Reuse array slots if possible)
-			id = $.fn.booklet.interfaces.length;
-			for(i = 0; i < id; i++)
-			{
-			   if(typeof $.fn.booklet.interfaces[i] == 'undefined'){ id = i; break; }
-			}
-			// Instantiate the booklet
-			obj = new booklet($(this), config, id);
-			// Add API references
-			$.fn.booklet.interfaces[id] = obj;
-		}
-	});
-}
-function booklet(target, options, id){
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-//VARS + STRUCTURE
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-	var self, opts, b, src,
-		hash, i, j, p, diff, busy, init, rhover, lhover,
-		titles = new Array(), chapters = new Array(),
-		pN, p0, p1, p2, p3, p4, pNwrap, p0wrap, p1wrap, p2wrap, p3wrap, p4wrap, wraps, sF, sB,
-		overlays, overlayN, overlayP, tabs, tabN, tabP, arrows, arrowN, arrowP, next, prev, ctrlsN, ctrlsP,
-		menu, chapter, dd, ddUL, ddH, ddLI, ddA, ddT, ddC, ddCUL, ddCH, ddCLI, ddCA, ddCT,
-		empty = '<div class="b-page-empty" title="" rel=""></div>', blank = '<div class="b-page-blank" title="" rel=""></div>'
-	;
-	busy         = false;
-	init         = false;
-	rhover = lhover = false;
-	self         = this;
-	self.options = options;
-	self.id      = id;
-	self.hash    = '';
-	opts         = self.options;
-	b            = target.addClass('booklet');
-	src          = b.children('.b-load');
-	//save page titles and chapter names, add page numbers
-	initPages();
-	//store data for api calls
-	b.data('booklet',true);
-	b.data('id', id);
-	b.data('total', src.children().length);
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-// SETUP OPTIONS
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-	//set width + height
-	if(!opts.width){
-		opts.width = b.width();
-	}
-	if(!opts.height){
-		opts.height = b.height();
-	}
-	b.width(opts.width);
-	b.height(opts.height);
-	//save page sizes and other vars
-	opts.pWidth  = opts.width/2;
-	opts.pWidthN = '-'+(opts.width/2)+'px';
-	opts.pWidthH = opts.width/4;
-	opts.pHeight = opts.height;
-	opts.pTotal  = src.children().length;
-	opts.speedH  = opts.speed/2;
-	//set startingPage
-	if(opts.direction == 'LTR'){
-		opts.curr = 0;
-	}else if(opts.direction == 'RTL'){
-		opts.curr = opts.pTotal-2;
-	}
-	if(!isNaN(opts.startingPage) && opts.startingPage <= opts.pTotal && opts.startingPage > 0){
-		if((opts.startingPage % 2) != 0){opts.startingPage--};
-		opts.curr = opts.startingPage;
-	}
-	//set booklet opts.name
-	if(opts.name){
-		document.title = opts.name;
-	}else{
-		opts.name = document.title;
-	}
-	//save shadow widths for anim
-	if(opts.shadows){
-		opts.shadowTopFwdWidth  = '-'+opts.shadowTopFwdWidth+'px';
-		opts.shadowTopBackWidth = '-'+opts.shadowTopBackWidth+'px';
-	}
-	//setup menu
-	if(opts.menu){
-		menu = $(opts.menu).addClass('b-menu');
-		p = opts.curr;
-		//setup page selctor
-		if(opts.pageSelector){
-			//add selector
-			dd = $('<div class="b-selector b-selector-page"><span class="b-current">'+ (p+1) +' - '+ (p+2) +'</span></div>').appendTo(menu);
-			ddUL = $('<ul></ul>').appendTo(dd).empty().css('height','auto');
-			//loop through all pages
-			for(i=0; i < opts.pTotal; i+=2){
-				j = i;
-				//nums for normal view
-				nums = (j+1) +'-'+ (j+2);
-				if(opts.closed){
-					//nums for closed book
-					j--;
-					if(i==0){nums='1'}
-					else if(i==opts.pTotal-2){nums=opts.pTotal-2}
-					else {nums = (j+1) +'-'+ (j+2);}
-					//nums for closed book with covers
-					if(opts.covers){
-						j--;
-						if(i==0){nums=''}
-						else if(i==opts.pTotal-2){nums=''}
-						else {nums = (j+1) +'-'+ (j+2);}
-					}
-				}
-				//nums for RTL direction
-				if(opts.direction == 'RTL'){
-					nums = (Math.abs(j - opts.pTotal)-1) +' - '+ ((Math.abs(j - opts.pTotal)));
-					if(opts.closed){
-						if(i==opts.pTotal-2){nums='1'}
-						else if(i==0){nums=opts.pTotal-2}
-						else{nums = (Math.abs(j - opts.pTotal)-3) +' - '+ ((Math.abs(j - opts.pTotal)-2));}
-						if(opts.covers){
-							if(i==opts.pTotal-2){nums=''}
-							else if(i==0){nums=''}
-							else{nums = (Math.abs(j - opts.pTotal)-5) +' - '+ ((Math.abs(j - opts.pTotal)-4));}
-						}
-					}
-					dd.find('.b-current').text(nums);
-					ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i+1] +'</span><span class="b-num">'+ nums +'</span></a></li>').prependTo(ddUL);
-				}else{
-					if(i==0){dd.find('.b-current').text(nums);}
-					ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i] +'</span><span class="b-num">'+ nums +'</span></a></li>').appendTo(ddUL);
-				}
-				ddA = ddLI.find('a');
-				if(!opts.hash){
-					ddA.click(function(){
-						if(opts.direction == 'RTL'){dd.find('.b-current').text($(this).find('.b-num').text());}
-						ddT = parseInt($(this).attr('id').replace('selector-page-',''));
-						self.gotoPage(ddT);
-						return false;
-					});
-				}
-			}
-			//set height
-			ddH = ddUL.height();
-			ddUL.css({'height':0, 'padding-bottom':0});
-			//add hover effects
-			dd.unbind('hover').hover(function(){
-				ddUL.stop().animate({height:ddH, paddingBottom:10}, 500);
-			},function(){
-				ddUL.stop().animate({height:0, paddingBottom:0}, 500);
-			});
-		}
-		//setup chapter selctor
-		if(opts.chapterSelector){
-			chapter = chapters[opts.curr];
-			if(chapter == ""){ chapter = chapters[opts.curr+1]; }
-			ddC = $('<div class="b-selector b-selector-chapter"><span class="b-current">'+chapter+'</span></div>').appendTo(menu);
-			ddCUL = $('<ul></ul>').appendTo(ddC).empty().css('height','auto');
-			for(i=0; i < opts.pTotal; i+=1){
-				if(chapters[i] != "" && typeof chapters[i] != "undefined"){
-					if(opts.direction == 'RTL'){
-						j = i;
-						if(j % 2 != 0){j--;}
-						ddC.find('.b-current').text(chapters[i]);
-						ddCLI = $('<li><a href="#/page/'+ (j+1) +'" id="selector-page-'+(j)+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').prependTo(ddCUL);
-					}else{
-						ddCLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').appendTo(ddCUL);
-					}
-					ddCA = ddCLI.find('a');
-					if(!opts.hash){
-						ddCA.click(function(){
-							if(opts.direction == 'RTL'){ddC.find('.b-current').text($(this).find('.b-text').text());}
-							ddCT = parseInt($(this).attr('id').replace('selector-page-',''));
-							self.gotoPage(ddCT);
-							return false;
-						});
-					}
-				}
-			}
-			ddCH = ddCUL.height();
-			ddCUL.css({'height':0, 'padding-bottom':0});
-			ddC.unbind('hover').hover(function(){
-				ddCUL.stop().animate({height:ddCH, paddingBottom:10}, 500);
-			},function(){
-				ddCUL.stop().animate({height:0, paddingBottom:0}, 500);
-			});
-		}
-	}
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-// API METHODS
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-	$.extend(self,
-	{
-		next : function(){
-			if(!busy){
-				self.gotoPage(opts.curr+2);
-			}
-		},
-		prev : function(){
-			if(!busy){
-				self.gotoPage(opts.curr-2);
-			}
-		},
-		gotoPage : function(num){
-			//moving forward (increasing number)
-			if(num > opts.curr && num < opts.pTotal && num >= 0 && !busy){
-				busy = true;
-				diff = num - opts.curr;
-				opts.curr = num;
-				opts.before.call(self, opts);
-				updatePager();
-				updateCtrls();
-				updateHash(opts.curr+1, opts);
-				initAnim(diff, true, sF);
-				//hide p2 as p3 moves across it
-				p2.stop().animate({width:0}, opts.speedH, opts.easeIn);
-				//animate p3 from right to left (left: movement, width: reveal slide, paddingLeft: shadow underneath)
-				//call setuppages at end of animation to reset pages
-				p3.stop().animate({left:opts.pWidthH, width:opts.pWidthH, paddingLeft: opts.shadowBtmWidth}, opts.speedH, opts.easeIn)
-				         .animate({left:0, width:opts.pWidth, paddingLeft:0}, opts.speedH);
-				p3wrap.animate({left:opts.shadowBtmWidth}, opts.speedH, opts.easeIn)
-					  .animate({left:0}, opts.speedH, opts.easeOut, function(){updateAfter()});
-			//moving backward (decreasing number)
-			}else if(num < opts.curr && num < opts.pTotal && num >= 0 && !busy){
-				busy = true;
-				diff = opts.curr - num;
-				opts.curr = num;
-				opts.before.call(self, opts);
-				updatePager();
-				updateCtrls();
-				updateHash(opts.curr+1, opts);
-				initAnim(diff, false, sB);
-				//hide p1 as p0 moves across it
-				p1.animate({left:opts.pWidth, width:0}, opts.speed, opts.easing);
-				p1wrap.animate({left:opts.pWidthN}, opts.speed, opts.easing);
-				//animate p0 from left to right (right: movement, width: reveal slide, paddingLeft: shadow underneath)
-				p0.animate({left:opts.pWidthH, width:opts.pWidthH}, opts.speedH, opts.easeIn)
-				  .animate({left:opts.pWidth, width:opts.pWidth}, opts.speedH, opts.easeOut);
-				//animate .wrapper content with p0 to keep content right aligned throughout
-				//call setuppages at end of animation to reset pages
-				p0wrap.animate({right:opts.shadowBtmWidth}, opts.speedH,opts. easeIn)
-					  .animate({right:0}, opts.speedH, opts.easeOut, function(){updateAfter()});
-			}
-		}
-	});
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-// SETUP CONTROLS
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-	//add prev next user defined controls
-	if(opts.next){
-		next = $(opts.next);
-		next.click(function(e){e.preventDefault(); self.next();});
-	}
-	if(opts.prev){
-		prev = $(opts.prev);
-		prev.click(function(e){e.preventDefault(); self.prev();});
-	}
-	//add overlays
-	if(opts.overlays){
-		overlayP = $('<div class="b-overlay b-overlay-prev b-prev" title="Previous Page"></div>').appendTo(b);
-		overlayN = $('<div class="b-overlay b-overlay-next b-next" title="Next Page"></div>').appendTo(b);
-		overlays = b.find('.b-overlay');
-		if ($.browser.msie) {
-			overlays.css({'background':'#fff','filter':'progid:DXImageTransform.Microsoft.Alpha(opacity=0) !important'});
-		}
-	}
-	//add tabs
-	if(opts.tabs){
-		tabP = $('<div class="b-tab b-tab-prev b-prev" title="Previous Page">Previous</div>').appendTo(b);
-		tabN = $('<div class="b-tab b-tab-next b-next" title="Next Page">Next</div>').appendTo(b);
-		tabs = b.find('.b-tab');
-		if(opts.tabWidth){
-			tabs.width(opts.tabWidth);
-		}
-		if(opts.tabHeight){
-			tabs.height(opts.tabHeight);
-		}
-		tabs.css({'top': '-'+tabN.outerHeight()+'px'});
-		b.css({'marginTop': tabN.outerHeight()});
-		//update ctrls for RTL direction
-		if(opts.direction == 'RTL'){
-			tabN.html('Previous').attr('title','Previous Page');
-			tabP.html('Next').attr('title','Next Page');
-		}
-	}else{
-		b.css({'marginTop': 0});
-	}
-	//add arrows
-	if(opts.arrows){
-		arrowP = $('<div class="b-arrow b-arrow-prev b-prev" title="Previous Page"><div>Previous</div></div>').appendTo(b);
-		arrowN = $('<div class="b-arrow b-arrow-next b-next" title="Next Page"><div>Next</div></div>').appendTo(b);
-		arrows = b.find('.b-arrow');
-		//update ctrls for RTL direction
-		if(opts.direction == 'RTL'){
-			arrowN.html('<div>Previous</div>').attr('title','Previous Page');
-			arrowP.html('<div>Next</div>').attr('title','Next Page');
-		}
-	}
-	//save all "b-prev" and "b-next" controls
-	ctrlsN = b.find('.b-next');
-	ctrlsP = b.find('.b-prev');
-	//add click actions
-	ctrlsN.click(function(e){e.preventDefault(); self.next();});
-	ctrlsP.click(function(e){e.preventDefault(); self.prev();});
-	//add page hover animations
-	if(opts.hovers){
-		ctrlsN.hover(
-			function(){
-				if(!busy && opts.curr+2 <= opts.pTotal-2){
-					//animate
-					p2.stop().animate({'width':opts.pWidth-40}, 500, opts.easing);
-					p3.stop().animate({'left':opts.width-40, 'width':20, paddingLeft: 10}, 500, opts.easing);
-					rhover = true;
-				}
-			},
-			function(){
-				if(!busy && opts.curr+2 <= opts.pTotal-2){
-					p2.stop().animate({'width':opts.pWidth}, 500, opts.easing);
-					p3.stop().animate({'left':opts.width, 'width':0, paddingLeft: 0}, 500, opts.easing);
-					rhover = false;
-				}
-			}
-		);
-		ctrlsP.hover(
-			function(){
-				if(!busy && opts.curr-2 >= 0){
-					//animate
-					p1.stop().animate({left:10, width:opts.pWidth-10}, 400, opts.easing);
-					p1wrap.stop().animate({left:"-10px"}, 400, opts.easing);
-					p0.stop().animate({left:10, width:40}, 400, opts.easing);
-					p0wrap.stop().animate({right:10}, 400, opts.easing);
-					lhover = true;
-				}
-			},
-			function(){
-				if(!busy && opts.curr-2 >= 0){
-					p1.stop().animate({left:0, width:opts.pWidth}, 400, opts.easing);
-					p1wrap.stop().animate({left:0}, 400, opts.easing);
-					p0.stop().animate({left:0, width:0}, 400, opts.easing);
-					p0wrap.stop().animate({right:0}, 400, opts.easing);
-					lhover = false;
-				}
-			}
-		);
-	}
-	//arrow animations
-	if(opts.arrows){
-		if($.support.opacity){
-			ctrlsN.hover(
-				function(){arrowN.find('div').stop().fadeTo('fast', 1);},
-				function(){arrowN.find('div').stop().fadeTo('fast', 0);
-			});
-			ctrlsP.hover(
-				function(){arrowP.find('div').stop().fadeTo('fast', 1);},
-				function(){arrowP.find('div').stop().fadeTo('fast', 0);
-			});
-		}else{
-			ctrlsN.hover(
-				function(){arrowN.find('div').show();},
-				function(){arrowN.find('div').hide();
-			});
-			ctrlsP.hover(
-				function(){arrowP.find('div').show();},
-				function(){arrowP.find('div').hide();
-			});
-		}
-	}
-	//keyboard ctrls
-	if(opts.keyboard){
-		//keyboard ctrls
-		$(document).keyup(function(event){
-			if(event.keyCode == 37){self.prev();}
-			else if(event.keyCode == 39){self.next();}
-		});
-	}
-	//hash ctrls
-	if(opts.hash){
-		setupHash();
-		clearInterval();
-		setInterval(function(){pollHash()}, 250);
-	}
-	//first time setup
-	resetPages();
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-//General Functions
-///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
-	function initPages(){
-		//fix for odd number of pages
-		if((src.children().length % 2) != 0){
-			//if book is closed and using covers, add page before back cover, else after last page
-			if(opts.closed && opts.covers){
-				src.children().last().before(blank);
-			}else{
-				src.children().last().after(blank);
-			}
-		}
-		//if closed book, add empty pages to start and end
-		if(opts.closed){
-			$(empty).attr({'title':opts.closedFrontTitle || "Beginning", 'rel':opts.closedFrontChapter || "Beginning of Book"}).prependTo(src);
-			src.children().last().attr({'title':opts.closedBackTitle || "End", 'rel':opts.closedBackChapter || "End of Book"});
-			src.append(empty);
-		}
-		if(opts.direction == 'LTR'){
-			j = 0;
-		}else{
-			j = src.children().length;
-			if(opts.closed){j-=2;}
-			if(opts.covers){j-=2;}
-			$(src.children().get().reverse()).each(function(){
-				$(this).appendTo(src);
-			});
-		}
-		//save titles and chapters
-		src.children().each(function(i){
-			//save chapter title
-			if($(this).attr('rel')){
-				chapters[i] = $(this).attr('rel');
-			}else{
-				chapters[i] = "";
-			}
-			//save page title
-			titles[i] = $(this).attr('title');
-			//give content the correct wrapper and page wrapper
-			if($(this).hasClass('b-page-empty')){
-				$(this).wrap('<div class="b-page"><div class="b-wrap"></div></div>');
-			}else if(opts.closed && opts.covers && (i == 1 || i == src.children().length-2)){
-				$(this).wrap('<div class="b-page"><div class="b-wrap b-page-cover"></div></div>');
-			}else if(i % 2 != 0){
-				$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-right"></div></div>');
-			}else{
-				$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-left"></div></div>');
-			}
-			$(this).parents('.b-page').addClass('b-page-'+i);
-			//add page numbers
-			if(opts.pageNumbers && !$(this).hasClass('b-page-empty') && (!opts.closed || (opts.closed && !opts.covers) || (opts.closed && opts.covers && i != 1 && i != src.children().length-2))){
-				if(opts.direction == 'LTR'){j++;}
-				$(this).parent().append('<div class="b-counter">'+(j)+'</div>');
-				if(opts.direction == 'RTL'){j--;}
-			}
-		});
-	}
-	function resetPages(){
-		//reset all content
-		b.find('.b-page').removeClass('b-pN b-p0 b-p1 b-p2 b-p3 b-p4').hide();
-		if(init){
-			j = opts.pTotal-1;
-			for(i=0;i<opts.pTotal;i++){
-				b.find('.b-page-'+i).detach().appendTo(src);
-			}
-		}
-		//add page classes
-		if(opts.curr-2 >= 0){
-			b.find('.b-page-'+(opts.curr-2)).addClass('b-pN').show();
-			b.find('.b-page-'+(opts.curr-1)).addClass('b-p0').show();
-		}
-		b.find('.b-page-'+(opts.curr)).addClass('b-p1').show();
-		b.find('.b-page-'+(opts.curr+1)).addClass('b-p2').show();
-		if(opts.curr+3 <= opts.pTotal){
-			b.find('.b-page-'+(opts.curr+2)).addClass('b-p3').show();
-			b.find('.b-page-'+(opts.curr+3)).addClass('b-p4').show();
-		}
-		//save structure elems to vars
-		pN     = b.find('.b-pN');
-		p0     = b.find('.b-p0');
-		p1     = b.find('.b-p1');
-		p2     = b.find('.b-p2');
-		p3     = b.find('.b-p3');
-		p4     = b.find('.b-p4');
-		pNwrap = b.find('.b-pN .b-wrap');
-		p0wrap = b.find('.b-p0 .b-wrap');
-		p1wrap = b.find('.b-p1 .b-wrap');
-		p2wrap = b.find('.b-p2 .b-wrap');
-		p3wrap = b.find('.b-p3 .b-wrap');
-		p4wrap = b.find('.b-p4 .b-wrap');
-		wraps  = b.find('.b-wrap');
-		//update css
-		wraps.attr('style','');
-		wraps.css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
-		p1.css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});
-		p2.css({'left':opts.pWidth, 'width':opts.pWidth, 'opacity':1, 'height':opts.pHeight});
-		pN.css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});
-		p0.css({'left':0, 'width':0, 'height':opts.pHeight});
-		p3.stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});
-		p3wrap.stop().css({'left':0});
-		p4.css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});
-		//update page order for animations
-		if(opts.curr+3 <= opts.pTotal){
-			p3.after(p0.detach());
-			p1.after(p4.detach());
-		}else{
-			p0.detach().appendTo(src);
-		}
-		init = true;
-		//add shadows
-		sF = sB = null;
-		b.find('.b-shadow-b, .b-shadow-f').remove();
-		if(opts.shadows){
-			sF = $('<div class="b-shadow-f"></div>').appendTo(p3).css({'right':0,'width':opts.pWidth, 'height':opts.pHeight});
-			sB = $('<div class="b-shadow-b"></div>').appendTo(p0).css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});
-		}
-	}
-	function initAnim(diff, inc, shadow){
-		//setup content
-		if(inc && diff > 2){
-			b.find('.b-p3, .b-p4').removeClass('b-p3 b-p4').hide();
-			b.find('.b-page-'+opts.curr).addClass('b-p3').show().stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});
-			b.find('.b-page-'+(opts.curr+1)).addClass('b-p4').show().css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});
-			b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
-			b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
-			p3     = b.find('.b-p3');
-			p4     = b.find('.b-p4');
-			p3wrap = b.find('.b-p3 .b-wrap');
-			p4wrap = b.find('.b-p4 .b-wrap');
-			if(rhover){
-				p3.css({'left':opts.width-40, 'width':20, 'padding-left': 10});
-			}
-			shadow.appendTo(p3);
-			p1.after(p4.detach());
-			p2.after(p3.detach());
-		}else if(!inc && diff > 2){
-			b.find('.b-pN, .b-p0').removeClass('b-pN b-p0').hide();
-			b.find('.b-page-'+opts.curr).addClass('b-pN').show().css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});
-			b.find('.b-page-'+(opts.curr+1)).addClass('b-p0').show().css({'left':0, 'width':0, 'height':opts.pHeight});
-			b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
-			b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
-			pN     = b.find('.b-pN');
-			p0     = b.find('.b-p0');
-			pNwrap = b.find('.b-pN .b-wrap');
-			p0wrap = b.find('.b-p0 .b-wrap');
-			if(lhover){
-				p0.css({left:10, width:40});
-				p0wrap.css({right:10});
-			}
-			shadow.appendTo(p0);
-			p0.detach().appendTo(src);
-		}
-		//updates if moving to start and end of book
-		if(opts.closed){
-			if(!inc && opts.curr == 0){
-				pN.hide();
-			}else if(!inc){
-				pN.show();
-			}
-			if(inc && opts.curr >= opts.pTotal-2){
-				p4.hide();
-			}else if(inc){
-				p4.show();
-			}
-		}
-		//init shadows
-		if(opts.shadows){
-			//check for opacity support -> animate shadow overlay on moving slide
-			if($.support.opacity){
-				shadow.animate({opacity:1}, opts.speedH, opts.easeIn)
-					  .animate({opacity:0}, opts.speedH, opts.easeOut);
-			}else{
-				if(inc){
-					shadow.animate({right:opts.shadowTopFwdWidth}, opts.speed, opts.easeIn);
-				}else{
-					shadow.animate({left:opts.shadowTopBackWidth}, opts.speed, opts.easeIn);
-				}
-			}
-		}
-	}
-	function updateAfter(){
-		resetPages();
-		updatePager();
-		updateCtrls();
-		opts.after.call(self, opts);
-		busy = false;
-	}
-	function updateCtrls(){
-		//update ctrls, cursors and visibility
-		if(opts.overlays || opts.tabs || opts.arrows){
-			if(opts.curr < opts.pTotal-2){
-				ctrlsN.fadeIn('fast').css('cursor',opts.cursor);
-			}else{
-				ctrlsN.fadeOut('fast').css('cursor','default');
-			}
-			if(opts.curr >= 2 && opts.curr != 0){
-				ctrlsP.fadeIn('fast').css('cursor',opts.cursor);
-			}else{
-				ctrlsP.fadeOut('fast').css('cursor','default');
-			}
-		}
-	}
-	function updatePager(){
-		if(opts.pageSelector){
-			if(opts.direction == 'RTL'){
-				nums = (Math.abs(opts.curr - opts.pTotal)-1) +' - '+ ((Math.abs(opts.curr - opts.pTotal)));
-				if(opts.closed){
-					if(opts.curr==opts.pTotal-2){nums='1'}
-					else if(opts.curr==0){nums=opts.pTotal-2}
-					else{nums = (Math.abs(opts.curr - opts.pTotal)-2) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-1));}
-					if(opts.covers){
-						if(opts.curr==opts.pTotal-2){nums=''}
-						else if(opts.curr==0){nums=''}
-						else{nums = (Math.abs(opts.curr - opts.pTotal)-3) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-2));}
-					}
-				}
-				$(opts.menu+' .b-selector-page .b-current').text(nums);
-			}else{
-				nums = (opts.curr+1) +' - '+ (opts.curr+2);
-				if(opts.closed){
-					if(opts.curr==0){nums='1'}
-					else if(opts.curr==opts.pTotal-2){nums=opts.pTotal-2}
-					else {nums = (opts.curr) +'-'+ (opts.curr+1);}
-					if(opts.covers){
-						if(opts.curr==0){nums=''}
-						else if(opts.curr==opts.pTotal-2){nums=''}
-						else {nums = (opts.curr-1) +'-'+ (opts.curr);}
-					}
-				}
-				$(opts.menu+' .b-selector-page .b-current').text(nums);
-			}
-		}
-		if(opts.chapterSelector){
-			if(chapters[opts.curr]!=""){
-				$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);
-			}else if(chapters[opts.curr+1]!=""){
-				$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);
-			}
-			if(opts.direction == 'RTL' && chapters[opts.curr+1]!=""){
-				$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);
-			}else if(chapters[opts.curr]!=""){
-				$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);
-			}
-		}
-	}
-	function setupHash(){
-		hash = getHashNum();
-		if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0 && hash != ''){
-			if((hash % 2) != 0){
-				hash--;
-			}
-			opts.curr = hash;
-		}else{
-			updateHash(opts.curr+1, opts);
-		}
-		self.hash = hash;
-	}
-	function pollHash(){
-		hash = getHashNum();
-		//check page num
-		if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0){
-			if(hash != opts.curr && hash.toString()!=self.hash){
-				if((hash % 2) != 0){hash--};
-				document.title = opts.name + " - Page "+ (hash+1);
-				if(!busy){
-					self.gotoPage(hash);
-					self.hash = hash;
-				}
-			}
-		}
-	}
-	//get page number from hash tag, last element
-	function getHashNum(){
-		var hash = window.location.hash.split('/');
-		if(hash.length > 1){
-			return parseInt(hash[2])-1;
-		}else{
-			return '';
-		}
-	}
-	//set the hash
-	function updateHash(hash, opts){
-		if(opts.hash){
-			window.location.hash = "/page/" + hash;
-		}
-	}
-}
-//define empty array to hold API references
-$.fn.booklet.interfaces = [];
-//define default options
-$.fn.booklet.defaults = {
-	name:               null,                            // name of the booklet to display in the document title bar
-	width:              600,                             // container width
-	height:             400,                             // container height
-	speed:              1000,                            // speed of the transition between pages
-	direction:          'LTR',                           // direction of the overall content organization, default LTR, left to right, can be RTL for languages which read right to left
-	startingPage:       0,                               // index of the first page to be displayed
-	easing:             'easeInOutQuad',                 // easing method for complete transition
-	easeIn:             'easeInQuad',                    // easing method for first half of transition
-	easeOut:            'easeOutQuad',                   // easing method for second half of transition
-	closed:             false,                           // start with the book "closed", will add empty pages to beginning and end of book
-	closedFrontTitle:   null,                            // used with "closed", "menu" and "pageSelector", determines title of blank starting page
-	closedFrontChapter: null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank starting page
-	closedBackTitle:    null,                            // used with "closed", "menu" and "pageSelector", determines chapter name of blank ending page
-	closedBackChapter:  null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank ending page
-	covers:             false,                           // used with  "closed", makes first and last pages into covers, without page numbers (if enabled)
-	pagePadding:        10,                              // padding for each page wrapper
-	pageNumbers:        true,                            // display page numbers on each page
-	hovers:             true,                            // enables preview pageturn hover animation, shows a small preview of previous or next page on hover
-	overlays:           true,                            // enables navigation using a page sized overlay, when enabled links inside the content will not be clickable
-	tabs:               false,                           // adds tabs along the top of the pages
-	tabWidth:           60,                              // set the width of the tabs
-	tabHeight:          20,                              // set the height of the tabs
-	arrows:             false,                           // adds arrows overlayed over the book edges
-	cursor:             'pointer',                       // cursor css setting for side bar areas
-	hash:               false,                           // enables navigation using a hash string, ex: #/page/1 for page 1, will affect all booklets with 'hash' enabled
-	keyboard:           true,                            // enables navigation with arrow keys (left: previous, right: next)
-	next:               null,                            // selector for element to use as click trigger for next page
-	prev:               null,                            // selector for element to use as click trigger for previous page
-	menu:               null,                            // selector for element to use as the menu area, required for 'pageSelector'
-	pageSelector:       false,                           // enables navigation with a dropdown menu of pages, requires 'menu'
-	chapterSelector:    false,                           // enables navigation with a dropdown menu of chapters, determined by the "rel" attribute, requires 'menu'
-	shadows:            true,                            // display shadows on page animations
-	shadowTopFwdWidth:  166,                             // shadow width for top forward anim
-	shadowTopBackWidth: 166,                             // shadow width for top back anim
-	shadowBtmWidth:     50,                              // shadow width for bottom shadow
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- * jQuery Booklet Plugin
- * Copyright (c) 2010 W. Grauvogel (http://builtbywill.com/)
- *
- * Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)
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- *
- * Version : 1.1.0
- *
- * Originally based on the work of:
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- */
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-/*
- * jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
- *
- * Uses the built in easing capabilities added In jQuery 1.1
- * to offer multiple easing options
- *
- * TERMS OF USE - jQuery Easing
- *
- * Open source under the BSD License.
- *
- * Copyright Â© 2008 George McGinley Smith
- * All rights reserved.
- *
- * Redistribution and use in source and binary forms, with or without modification,
- * are permitted provided that the following conditions are met:
- *
- * Redistributions of source code must retain the above copyright notice, this list of
- * conditions and the following disclaimer.
- * Redistributions in binary form must reproduce the above copyright notice, this list
- * of conditions and the following disclaimer in the documentation and/or other materials
- * provided with the distribution.
- *
- * Neither the name of the author nor the names of contributors may be used to endorse
- * or promote products derived from this software without specific prior written permission.
- *
- * THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
- * EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
- * MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
- *  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
- *  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
- *  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
- * AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
- *  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
- * OF THE POSSIBILITY OF SUCH DAMAGE.
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-c.css(a,"position")==="static";)a=a.offsetParent;return a})}});c.each(["Left","Top"],function(a,b){var d="scroll"+b;c.fn[d]=function(e){var f=this[0],h;if(!f)return null;if(e!==B)return this.each(function(){if(h=fa(this))h.scrollTo(!a?e:c(h).scrollLeft(),a?e:c(h).scrollTop());else this[d]=e});else return(h=fa(f))?"pageXOffset"in h?h[a?"pageYOffset":"pageXOffset"]:c.support.boxModel&&h.document.documentElement[d]||h.document.body[d]:f[d]}});c.each(["Height","Width"],function(a,b){var d=b.toLowerCase();
-c.fn["inner"+b]=function(){return this[0]?parseFloat(c.css(this[0],d,"padding")):null};c.fn["outer"+b]=function(e){return this[0]?parseFloat(c.css(this[0],d,e?"margin":"border")):null};c.fn[d]=function(e){var f=this[0];if(!f)return e==null?null:this;if(c.isFunction(e))return this.each(function(l){var k=c(this);k[d](e.call(this,l,k[d]()))});if(c.isWindow(f))return f.document.compatMode==="CSS1Compat"&&f.document.documentElement["client"+b]||f.document.body["client"+b];else if(f.nodeType===9)return Math.max(f.documentElement["client"+
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-/* @Booklet
-----------------------------------------*/
-	.booklet           {width:800px; height:600px; position:relative; /*background:#fff;*/ margin:0 auto 10px;}
-	.booklet .b-page  {left:0; top:0; position:absolute; overflow:hidden; padding:0;}
-	.booklet .b-pN  {}
-	.booklet .b-p0  {}
-	.booklet .b-p1  {}
-	.booklet .b-p2  {}
-	.booklet .b-p3  {}
-	.booklet .b-p4  {}
-	/*.booklet .b-load  {display:none;}*/
-   	/* Page Wrappers */
-	.booklet .b-wrap       {top:0; position:absolute;}
-	.booklet .b-wrap-left  {background:#fff;}
-	.booklet .b-wrap-right {background:#efefef;}
-	.booklet .b-pN .b-wrap,
-	.booklet .b-p1 .b-wrap,
-	.booklet .b-p2 .b-wrap,
-	.booklet .b-p3 .b-wrap,
-	.booklet .b-p4 .b-wrap  {left:0;}
-	.booklet .b-p0 .b-wrap  {right:0;}
-   	/* Custom Page Types */
-	.booklet .b-page-blank  {padding:0; width:100%; height:100%;}
-	.booklet .b-page-cover  {padding:0; width:100%; height:100%; background:#925C0E;}
-	.booklet .b-page-cover h3  {color:#fff; text-shadow:0px 1px 3px #222;}
-   	/* Page Numbers */
-	.booklet .b-counter {bottom:10px; position:absolute; display:block; width:25px; height:20px; background:#ccc; color:#444; text-align:center; font-family:Georgia, "Times New Roman", Times, serif; font-size:10px; padding:5px 0 0;}
-	.booklet .b-wrap-left  .b-counter  {left:10px;}
-	.booklet .b-wrap-right .b-counter {right:10px;}
-   	/* Page Shadows */
-	.booklet .b-shadow-f  {right:0; top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-forward.png"); background-repeat:repeat-y; background-position:100% 0;}
-	.booklet .b-shadow-b  {left:0;  top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-back.png");    background-repeat:repeat-y; background-position:0 0;}
-	.booklet .b-p0 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:100% 10px;}
-	.booklet .b-p3 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:0 10px;}
-   	/* Overlay Controls */
-	.booklet .b-overlay      {top:0; position:absolute; height:100%; width:50%;}
-	.booklet .b-overlay-prev {left:0;}
-	.booklet .b-overlay-next {right:0;}
-   	/* Tab Controls */
-	.booklet .b-tab        {background:#000; height:40px; width:100%; padding:10px 0 0; text-align:center; color:#ccc; position:absolute; font-size:11px;}
-	.booklet .b-tab-prev   {left:0;}
-	.booklet .b-tab-next   {right:0;}
-	.booklet .b-tab:hover  {background:#aaa; color:#000;}
-   	/* Arrow Controls */
-	.booklet .b-arrow          {display:block; position:absolute; text-indent:-9999px; top:0; height:100%; width:74px;}
-	.booklet .b-arrow div      {display:none; text-indent:-9999px; height:170px; width:74px; position:absolute; left:0; top:25%;}
-	.booklet .b-arrow-next     {right:-80px;}
-	.booklet .b-arrow-prev     {left:-80px;}
-	.booklet .b-arrow-next div {background:url("images/arrow-next.png") no-repeat left top;}
-	.booklet .b-arrow-prev div {background:url("images/arrow-prev.png") no-repeat left top;}
-/* @z-index fix (needed for older IE browsers)
-----------------------------------------*/
-	body              {z-index:0;}
-    .b-menu           {z-index:100;}
-    .b-selector       {z-index:100;}
-	.booklet          {z-index:10;}
-    .b-pN             {z-index:10;}
-    .b-p0             {z-index:10;}
-    .b-p1             {z-index:10;}
-    .b-p2             {z-index:10;}
-    .b-p3             {z-index:10;}
-    .b-p4             {z-index:10;}
-    .b-prev           {z-index:40;}
-    .b-next           {z-index:40;}
-    .b-counter        {z-index:40;}
-/* @Menu Items
-----------------------------------------*/
-	.b-menu {height:40px; padding:0 0 10px;}
-	.b-selector             {height:40px; position:relative; float:right; border:none; color:#cecece; cursor:pointer;}
-	.b-selector .b-current  {padding:8px 15px 12px; line-height:20px; min-width:18px; height:20px; display:block; background:#000; text-align:center;}
-	.b-selector-page        {width:auto; margin-left:15px;}
-	.b-selector-chapter     {width:auto;}
-	.b-selector:hover            {color:#fff; background-position:left 0px;}
-	.b-selector:hover .b-current {background-position:right 0px;}
-	.b-selector ul               {overflow:hidden; margin:0; list-style:none !important; position:absolute; top:40px; right:0; padding:0 0 10px; background:#000; width:240px; font:normal 12px "Myriad Pro", Myriad, "DejaVu Sans Condensed","Liberation Sans", "Nimbus Sans L", Tahoma, Geneva, "Helvetica Neue", Helvetica, Arial, sans-serif;}
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-<body>
-<h1 class="title">              Protocols //Notebook </h1>
-    <div class="book_wrapper"> <a id="next_page_button"></a> <a id="prev_page_button"></a>
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-          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/8/88/FotoiGEM1.jpg" />
-            <h1>Protocol 1 </h1>
-            <p>Preparation of culture media (1L).<br />
-.- 5 g of yeast<br />
-.- 10 g of sodium chloride.<br />
-.- 10 g of triptone.<br />
-.- Add water till 1 uL volume.</p>
-          </div>
-          <div>
-            <h1>Protocol 2</h1>
-            <p>Competent cell´s preparation<br />
-              <br />
-.- Centrifug your media culture tube (25 mL) at 4,000 rpm during
-min.<br />
-.- Pour the supernatant and add 10 mL of cold MgCl2 solution.
-              Centrifuge them at 4,000 rpm at 4 °C during 15 min.<br />
-.- Pour the supernatant and resuspend the pellet in 40 mL of cold
-              CaCl2 solution. Keep it in ice during 20 min and place them in
-              eppendorf tubes. Centrifuge them at 4,000 rpm at 4°C during 15
-              min.<br />
-.- Pour the supernatant and resuspend the pellet in 40 mL of cold
-mM CaCl2/15% glycerlol v/v solution. Centrifuge again at 2,100
-              rpm at 4°C during 15 min.<br />
-.- Pour the supernatant and resuspend in 2 mL of cold 85bmM
-              CaCl2/15% glycerol v/v solution.<br />
-.- Aliquote in tubes of 100 uL previously frozen and store at
-              -80°C.<br />
-            </p>
-          </div>
-          <div>
-            <h1>Protocol 3</h1>
-            <p>DNA extraction from Buffy coat:<br />
-              <br />
-              <font size="1"> 1.- Centrifuge 5 mL of culture medium at 1,500 rpm
-                for 10 min at 4°C.</font><br />
-              <font size="1">2.- Carefully transfer 600 uL of buffy coat to a
-                microtube.</font><br />
-              <font size="1">3.- Add 1 mL of RBC buffer and mix throughly by
-                vortex.</font><br />
-              <font size="1">4.- Centrifuge at 4,500 rpm during 30 s and pour
-                carefully so your pellet is not wasted.</font><br />
-              <font size="1">5.- Repeat steps 2 and 3 adding just 500 uL from
-                RBC buffer.</font><br />
-              <font size="1">6.- Once separated, add 300 uL of lysis buffer to
-                the pellet and mix by vortex.</font><br />
-              <font size="1">7.- Add 150 uL of protein precipitation solution.</font><br />
-              <font size="1">8.- Centrifuge at 11,000 rpm during 5 min; then,
-                transfer the supernatant to a new microtube.</font><br />
-              <font size="1">9.- Add 700 uL of cold isopropanol and mix by
-                inverting until you see a white formation within the tube.</font><br />
-              <font size="1">10.- Centrifuge at 11,000 rpm during 4 min and pour
-                carefully taking care of the pellet at the bottom.</font><br />
-              <font size="1">11.- Add 500 uL of cold ethanol 70% to wash and
-                then centrifuge at 11,000 rpm during 4 min.</font><br />
-              <font size="1">12.- Pour and let the ethanol evaporate at room
-                temperature.</font><br />
-              <font size="1"> 13.- Resuspend the DNA pellet with 12 uL of TE
-                buffer; incubate all night at 4 °C.</font><br />
-            </p>
-          </div>
-          <div>
-            <h1>Protocol 4</h1>
-            <p>Electrophoresis:<br />
-              <br />
-              <font size="1">1.- Prepare 1L of TAE 10X (48.4g of Tris base[ tris
-                (hidroxymethyl) amminomethane], 11.4 mL of glacial acetic acid
-                (17.4 M) and 3.7 g of EDTA then fill with deionized water to 1
-                L.</font><br />
-              <font size="1">2.- Prepare a 50 mL solution of 3% agarose using
-                TAE buffer (without using water).</font><br />
-              <font size="1">3.- Heat the gel in the microwave in intervals of
-s, 20 s and 10 s until the agarose is completely dissolved,
-                taking care that it does not boil so much.</font><br />
-              <font size="1">4.- Let cool down the gel (55-60°C) and pour in a
-                base with the comb already on its place to form the charging
-                wells.</font><br />
-              <font size="1">5.- Wait until the gel solidifies.</font><br />
-              <font size="1">6.- Remove the comb and place the base with the gel
-                inside the electrophoretic chamber.</font><br />
-              <font size="1">7.- Add TAE buffer until the gel is covered.</font><br />
-              <font size="1">8.- Take 5 uL of each sample from the PCR and 1 uL
-                of charging gel in a new PCR tube, mix well.</font><br />
-              <font size="1">9.- Charge the samples inside the wells of the gel,
-                adding a weight marcker.</font><br />
-              <font size="1">10.- Close the electrophoretic chamber and run at
-V during 30 min. Follow the displacement ofthe samples.</font><br />
-              <font size="1">11.- Take out the gel from the chamber very
-                carefully and place in the UV light to see the DNA strands.</font><br />
-            </p>
-          </div>
-          <div>
-            <h1>Protocol 5</h1>
-            <p>Competent cell transformation:<br />
-              <br />
-.- Place 1 uL of the plasmid in a microtube, gently add 100 uL of
-              your competent cells. Take another tube and place only 100 uL of
-              competent cells, so you have a control group.<br />
-.- Mix gently with a pipette and incubate during 30 min in ice.<br />
-.- Ab ruptly place your cells in 42°C dry bath during 2 min and
-              replace in ice whwn finished.<br />
-.- Add 900 uL of LB medium and incubate at 37 °C for 30 min.<br />
-.- Inoculate with 200 uL of your transformed cells in LB-Amp.<br />
-.- Incubate overnight and store at 4 °C of freeze in 15-25%
-              glycerol.<br />
-            </p>
-          </div>
-          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/e/e4/LabArchives.jpg" />
-            <h1>Lab archives// Mar 12, 2014 </h1>
-            <p>In order to grow our bacteria, we inoculated with 100 uL of E.
-              Coli DH5-alpha in 50 mL of LB medium, and 100 uL of E. Coli TOP in
-mL of LB medium. We left both samples incubating overnight </p>
-            <h1>Lab archives// Mar 20, 2014 </h1>
-            <p>We inoculated E.Coli NEB10-beta in 50 ml of LB medium. </p>
-          </div>
-          <div>
-            <h1>Lab archives// Mar 27, 2014 </h1>
-            <p>We prepared the medium in which our bacteria will grow, for that
-              we dissolved solid LB with agarose, then we add it into 10 plates,
-              we waited a few minutes for it to solidify and finally we added
-uL of our antibiotic that is chloranphenicol to each of those
-              plates. </p>
-            <h1>Lab archives// Apr 01, 2014 </h1>
-            <p>we transformed luciferaze from DNA to cells by incerting them
-              with change of temperature </p>
-            <h1>Lab archives// Apr 02, 2014 </h1>
-            <p>We made a litter of agar and 500 L of growth medium, and we
-              sterilized them along with crystal media disperssion balls </p>
-            <h1>Lab archives// Apr 03, 2014 </h1>
-            <p>Today we prepared buffers for home made mini preps and did a cell
-              re culture. </p>
-          </div>
-          <div>
-            <h1><font size="1">Lab archives// Apr 07, 2014 </font> </h1>
-            <p> <font size="1">We added 700 mL of water in a beaker and then we
-                prepared 23 grams of powdered agar that we added slowly while we
-                moved the beaker. This in order to help the dissolve the powder.
-                After that, we added other 300 mL to the solution and finally we
-                passed it to an Erlenmeyer matrass. Also we prepared 500 mL of
-                growth medium. First, we measured and added 5 grams of tryptone,
-grams of yeast and 10 grams of sodium chloride. Those
-                ingredients were added slowly to a graduated cylinder with 350
-                mL of wáter while the flask was moved. After finishing the
-                addition we added other 150 mL of water. The solution was then
-                transfered to another Erlenmeyer flask. Both Erlenmeyer flasks
-                and a glass bottle with crystal media diperssion balls were
-                sterilized with temperature and pressure.</font> </p>
-            <h1><font size="1">Lab archives// Apr 02, 2014 </font> </h1>
-            <p><font size="1">300 ul of ampicillin at 100mg/ml chloramphenicol </font><br />
-              <font size="1"> 3 Plates with agar </font><br />
-              <font size="1">Promoters: BBa_K258005 (pO2), BBa_176500 (pFe)
-                BBa_J52008 (luc-3) </font> <br />
-              <font size="1">The plates were impregnated with ampicillin
-                promoting bacteria in the following manner </font><br />
-              <font size="1"> |pO2 | Plate | 150 ul Ampiciline | K258005 | </font><br />
-              <font size="1">
-                ------------------------------------------------------ </font><br />
-              <font size="1"> |pFe | Plate | 150 ul Ampiciline | 1765000 | </font><br />
-              <font size="1">
-                ------------------------------------------------------ </font><br />
-              <font size="1"> |Luc-3 | Plate | Chloranphenicol | J52008 | </font><br />
-            </p>
-            <p></p>
-          </div>
-          <div>
-            <h1><font size="1">Apr 08, 2014 </font></h1>
-            <p> <font size="1"> **/Today we realized the mini prep for the
-                oxygen promoter. /** </font><br />
-              <font size="1"> --500 ul of Ampiciline //this is for the oxygen
-                promoter </font><br />
-              <font size="1"> --50 ul of oxygen promoter </font><br />
-              <font size="1"> --45 ml of LB medium </font><br />
-              <font size="1"> --at room temperature 15 minutes at 1500 rpm in
-                the centrifuge. </font><br />
-              <font size="1"> --500 ul of chloramphenicol // this is for the red
-                liciferase </font><br />
-              <font size="1"> --50 ul of red luciferase </font><br />
-              <font size="1"> --45 ml of LB medium </font><br />
-              <font size="1"> --The new LB medium was prepared with //LB medium
-              </font><br />
-              <font size="1"> --10 g yeast </font><br />
-              <font size="1"> --5 g salt </font><br />
-              <font size="1"> --15 g Tryptone </font> <font size="1"> MINI
-                PREP First 400 ul of Genomic Lysis Buffer to the 40 ml overnight
-                [bacteriaand oxygen promoter] Then the mixture is transferred to
-                a Zyne-Spine Collumn in a Collection Tube. Centrifuge 10,000 rpm
-                /1 min Transfer the Zyno Spin Column to a new Collection Tube.
-                Add 200 micro liters of Pre Wash Buffer. Centrifuge 10,000 rpm
-                /1 min Add 500 uL of of g-DNA Wash buffer to the spin column.
-                Centrifuge 10,000 rpm /1 min Transfer the spin column to a clear
-                micro centrifuge tube. Add 50 uL of DNA Elution Buffer .
-                Incubate 2-5 minutes to at room temperature. Centrifuge at top
-                speed per 30 seconds. Then store at -80 °C </font></p>
-          </div>
-          <div>
-            <h1><font size="1"> Apr 22, 2014</font> </h1>
-            <p><font size="1"> We prepared 2 overnight cultures (45ml of LB
-                medium): GFP E0040 pSB1A2 with 100 uL of ampicillin. M-cherry
-                (RFP) BBa_J04450 pSB1C3 with 500 uL of chloramphenicol.</font></p>
-            <h1><font size="1"> Apr 24, 2014 </font></h1>
-            <p><font size="1"> 2 Transformations: Bacteria NEB 10 Beta, DH5alpha
-Mini Preps: GFP, RFP //Transformation and preps:--
-                Centrifugation at 2500 rpm for 15 min.--1ul DNA in 100 uL of
-                bacterial culture (luciferase). --Filtering GFP, RFP bacteria.
-                --Added 400 ml of Lysis Buffer Genomyc each crop by columns.
-                --Centrifugation at 10,000 * g for 1 min. --Last column to
-                another tube and DNA Prewash + 200 uL Buffer. --Centrifuged at
-,000 * g for 1 min. --Add 500ul of DNA Wash Buffer,
-                centrifuged at 10,000 * g for 1 min. --Transfer to clean tube,
-                add 50ul of DNA Elution Buffer. --Incubate for 2-5 min at room
-                temperature, centrifuged at maximum speed for 30 sec.</font></p>
-            <h1><font size="1"> Apr 28, 2014 </font></h1>
-            <p><font size="1"> Today was prepared LB media. It also was done the
-                sterilization of 1 mL and 200 uL tips, water and ependorfs.
-                After the sterilization of the LB media was done, we cultivated
-                in it the bacteria carrying the oxygen promoter, RFP, and GFP.
-                Then we left the bacteria growing in the incubator at 37°C
-                overnight.</font></p>
-          </div>
-          <div>
-            <h1><font size="1"> May 05, 2014 </font></h1>
-            <p><font size="1"> Plating bacteria with GFP, RFP, promoter Oxygen
-                and iron promoter plasmids. Everyone in their respective
-                environments and specific antibiotics (ampicillin and
-                chloramphenicol). Tubes with bacteria was added and placed them
-                in LB medium incubator to grow more product if necessary.</font></p>
-            <h1><font size="1"> May 08, 2014 </font></h1>
-            <p><font size="1"> We did a centrifuge at 4800 rpm for 20 min for
-                the plasmids had been in overnight which had oxygen promoter,
-                GFP and RFP to rush after we separate the aqueous phase.</font></p>
-            <h1><font size="1"> May 19, 2014 </font></h1>
-            <p><font size="1"> We did inoculation of GFP, RFP and Oxigen Prmoter</font></p>
-            <h1><font size="1"> May 20, 2014 </font></h1>
-            <p><font size="1"> We started minipreps preparation of GFP, RFP and
-                Oxigen Promoter. We added 1.5 ml of culture to each Eppendorff
-                (4 for each promoter) and we centrifugated it at 13.2 rpm for 10
-                minutes, we did the same procedure 2 times. Also, we sterilized
-                two Erlenmeyer flasks and 40 Eppendorffs. </font></p>
-          </div>
-          <div>
-            <h1><font size="1"> May 20, 2014 //Mini prep preparation </font></h1>
-            <p><font size="1"> 1. We added 1.5 mL of the cultures to their
-                corresponding Eppendorffs and centrifugated them at 3400 rpm
-                during 10 minutes other three times.</font></p>
-            <p><font size="1">2. Once we had a pellet we discarded the
-                supernatant and we added 300 uL of TEG buffer (made of 100 mM of
-                Tris-HCl pH8, 2 mM EDTA and 20% glucose)</font></p>
-            <p><font size="1">3. We added 700 μL of NS solution (0.2 M NaOH, 1%
-                w/v SDs) and we mixed by inversion.</font></p>
-            <p><font size="1">4. We added 400 μL of 3 M sodium acetate pH 5.3,
-                and we mixed by inversion.</font></p>
-            <p><font size="1">5. We centrifugated the Eppendorffs at 3400 rpm
-                during 15 minutes.</font></p>
-            <p><font size="1">6. We transfered the supernatant to other
-                Eppendorffs and we added 50 uL of cold isopropanol and mixed by
-                inversion.</font></p>
-            <p><font size="1">7. We centrifugated the Eppendorffs at 3400 rpm
-                during 15 minutes.</font></p>
-            <p><font size="1">8. We discarded the supernatant and washed each
-                pellet with 70% ethanol and we let it dry for 15 minutes.</font></p>
-            <p><font size="1">9. We resuspended the pellet in 500 uL of TE
-                buffer and then we added 10 mg/mL RNAseA.</font></p>
-            <p><font size="1">11. We precipitated the DNA with 100 uL of ethanol
-                in each Eppendorff.</font></p>
-            <p><font size="1">12. We centrifugated for 1 minute at 3400 rpm.</font></p>
-            <p><font size="1">13. We got rid of the supernatant.</font></p>
-            <p><font size="1">14. Finally, we resuspended the pellet in 50 uL of
-                TE buffer</font></p>
-          </div>
-          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/f/f4/1%281%29.jpg" />
-            <h1>UNAM procedures// Digestion</h1>
-            <p>|BSA | 1 uL | </p>
-            <p>|DNA | 2 uL | </p>
-            <p>|Buffer (2) | 1 uL |</p>
-            <p>|Buffer Xba I+Pstl | 3.3 uL |</p>
-            <p>|fe Xba I+Spef | 3.3 uL | </p>
-            <p>|H<sub>2</sub>0 | 5.4 uL | </p>
-          </div>
-          <div>
-            <h1><font size="1">Gel agarose</font></h1>
-            <p><font size="1"> 1 kb NEB Ampyciline -&gt; Pentneryl </font></p>
-            <p><font size="1">| ---------------Bristol - Myers Squibb</font> </p>
-            <p><font size="1">| ---------------1 gram/ 3 mL</font> </p>
-            <p><font size="1">| ---------------inyectable</font></p>
-            <p><font size="1">|<sub>p</sub>O <sub>2</sub> | 3.3 uL</font> </p>
-            <p><font size="1">|1 kb marker 5.4 uL </font></p>
-            <h1><font size="1">Homemade Mini prep Preparation </font></h1>
-            <p><font size="1"> 1.- Overnight bacterial culture of 5 mL in
-                falkons of 50 mL </font></p>
-            <p><font size="1"> 2.- Centrifugate in an eppendor of 1.5 mL and
-                throw supernadant</font></p>
-            <p><font size="1"> 3.- Add 152 uL of Buffer 1 + RNAse (11
-                micro-liters /1 buffer micro-liter ) **Buffer 20% Glucouse </font></p>
-            <p><font size="1"> 4.- Mix via Buffer and add 150 uL of Buffer 2
-                *Wait 4-5 minutes*</font> </p>
-            <p><font size="1"> (1.5mL)Buffer 2 -&gt; 880 uL H<sub>2</sub>O,20uL
-                NaOH 10 N </font></p>
-            <p><font size="1"> ---------------------------------------100 uL SDS
-%</font> </p>
-            <p><font size="1"> 5.- Mix via inversion and add150 uL Buffer 3 </font>
-            </p>
-            <p><font size="1"> ------------------------------Sodium Acetate 3M
-                pH 5</font> </p>
-          </div>
-          <div>
-            <p><font size="1"> 6.-Centrifuge 5 minutes.</font> </p>
-            <p><font size="1"> 7.-Take the supernadant to antoher tube. Add 1 mL
-                ETOH Absolute </font></p>
-            <p><font size="1"> 8.-Centrifuge by 5 minutes and decant </font> </p>
-            <p><font size="1"> 9.-Wash with 500uL ETOH 70 %</font> </p>
-            <p><font size="1"> 10.-Centrifugate 1 minute </font></p>
-            <p><font size="1"> 11.-Decant and dry at 37 celsius centigrades (30
-                minutes)</font> </p>
-            <p><font size="1"> 12.-Resuspend in 15 uL of H<sub>2</sub>O </font></p>
-            <h1><font size="1">Ligation</font></h1>
-            <p><font size="1"> Inserto 7mL</font></p>
-            <p><font size="1"> Plasmid 3mL</font> </p>
-            <p><font size="1"> Buffer 10x 1.5mL </font> </p>
-            <p><font size="1"> Ligase 0.5 mL </font></p>
-            <p><font size="1"> H<sub>2</sub>O 3 mL </font> </p>
-            <p><font size="1"> Total = 15 ml </font></p>
-          </div>
-          <div>
-            <h1>E.Coli cells transformation. </h1>
-            <p>To transform E.Coli cells with an exogen DNA, 200 uL of competent
-              cells were taken they were mixed with 1-20 ug(Micrograms) of DNA
-              that was incubated on ice for 30 minutes. This makes posible the
-              absorbtion of the exogen DNA to the cells surface, after that, the
-              cells are incubated at 42Celsius Degrees during 90 seconds, for
-              then incubate them during 1 minute again in ice. THis termal shock
-              makes posible the entrance of the plasmid to the inside of the
-              cell. 600 uL of LB medium were added and then incubated during
--90 minutes at 37 Celisus Degres, to do posible the segregation
-              of the exogenic plasmid during the celular division (3 to 5
-              generations). Finally, Alicuote of 100-200 uL were cultivated by
-              dimention in the LB agar medium, which ere incubated at 37 Celsius
-              Degrees. After 15-20 hours of incuvation colonies could be
-              observed</p>
-          </div>
-          <div>
-            <h1>Ligations</h1>
-            <p>1# Grow h mL in overnight</p>
-            <p>2# Do minipreps</p>
-            <p>3# EcoRI and PstI restrictions.</p>
-            <p> --for Fe EcoRIand SpeI</p>
-            <p>O<sub>2</sub>+ GFP ---1,2,3 - 931</p>
-            <p>Fe+GTP ---4,5,6 -1,828</p>
-            <p>O<sub>2</sub>+ RFP ---7,8,9,10,11,12,13 -890</p>
-            <p>Fe+RFP ---14,15,16,17,18,19 -1,787</p>
-            <br />
-            <p>GFP -&gt; 761 bp EcoRI Po+I // 728 bp XbaI SpeI</p>
-            <p>RFP -&gt; 714 bp Xba - SpeI // 747 bp EcoR - Ps+I</p>
-            <br />
-            <p>pFe -&gt; 1,067 bp EcoRI - SpeI </p>
-            <p><sub>p</sub>O<sub>2</sub> -&gt; 178 bp EcoRi SpeI // 145 bp Xba -
-              Ps+I </p>
-          </div>
-          <div>
-            <p>O<sub>2</sub> + GFP 1/1 - 1/2 - 3/3</p>
-            <p>FO <sub>2</sub> + GFP 4/1 - 5/2 - 6/3</p>
-            <p>O<sub>2</sub> + RFP 7/1 - 8/2 - 9/3 - 10/4 - 11/5 - 12/6 - 12/13
-            </p>
-            <p>Fe + RFP 13/1 - 14/2 - 15*16/3 - 17/4 - 18/5 -19/6</p>
-            <h1>Digestion</h1>
-            <p>2 --- BSA --- 1uL</p>
-            <p>4 --- DNA --- 2uL</p>
-            <p>3 --- (M) Buffer // Enz (0.3 uL each one)--- 1uL</p>
-            <p>5 --- EcoRI --- 0.3 uL</p>
-            <p> --- PstI --- 0.3 uL</p>
-            <p>1 --- H<sub>2</sub>O --- 5.4 uL</p>
-            <p> Total == 10 uL rxn</p>
-            <br />
-            <p> GFP 761 bp</p>
-            <p> RFP 742 bp</p>
-          </div>
-          <div>
-            <p> pFe 1,067 bp </p>
-            <p> pO<sub>2</sub> -15.5 bp</p>
-            <br />
-            <p> pO<sub>2</sub> + GFP 931 bp</p>
-            <p> pFe + GFP 1,828 bp</p>
-            <p> pO<sub>2</sub>+ RFP 890 bp</p>
-            <p> pFe + RFP 1,787 bp</p>
-            <br />
-            <p> Gel 1 = 8/9/10/Lodder 1kb/11/12/13</p>
-            <p> Gel 2 = 15/16/17/Lodder 116/18/19</p>
-            <p> Gel 3 = 1/2/3/4/Lodder 1kb/5/6/7</p>
-          </div>
-        </div>
-      </div>
-    </div>
-    <div>  <span class="reference"> </span> </div>
-</body>
-</html>

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Latest revision as of 03:20, 21 June 2014