Team:CSWProteens

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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<center><a href="https://2014hs.igem.org/Team:CSWProteens/project"><img alt="" class="auto-style11" height="200" src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="200" /><img src="https://static.igem.org/mediawiki/2014hs/7/7e/CSW_Banner.png" width="500">
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<ul id="nav">
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<li><a href="https://2014hs.igem.org/Team:CSWProteens"><b>Home</b></a></li>
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  <li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/team"><b>Team</b></a>
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    <ul>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/team">Members</a></li>
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        <li><a href="http://www.csw.org/">The Cambridge School of Weston</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/advisors">Advisors</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/sponsors">Sponsors</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/Attributions">Attributions</a></li>
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</ul>
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<a href="https://2014hs.igem.org/Team:CSWProteens/project"><b>Project</b></a>
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<ul>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/frostprotection">Frost Protection</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project">Project Description</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/Methods">Methods</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/results">Results</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/discussion">Discussion</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/safety">Safety</a></li>
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</ul>
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</li>
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<li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><b>Notebook</b></a>
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<ul>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/">Lab Journal</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/protocols">Protocols</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/parts">Parts</a></li>
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                                <li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/references">References</a></li>
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</ul>
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<li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/humanpractices"><b>Outreach</b></a>
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<ul>
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You may also wish to add other page such as:
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<a href="https://2014hs.igem.org/Team:CSWProteens/contact"><b>Contact Us</b></a>
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<li>sponsor information</li>
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<ul>
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<li><a href="http://csw-igem.tumblr.com">Tumblr</a></li>
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<li><a href="http://www.facebook.com/cswigem">Facebook</a></li>
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REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:CSWProteens/Our_Pets</span>
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                                <li><a href="mailto:hgoldsweig@csw.org?subject=iGEM">Email</a></li>
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<h3><p>Welcome!<br> We are the CSW Proteens! <br>Scroll down to find more about our project! </center></h3>
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|align="center"|[[Team:CSWProteens | Team CSWProteens]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=CSWProteens Official Team Profile]
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<h3>PlantiFreeze</h3>
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<p>Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.</p>
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===Team===
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===Project===
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Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly,  and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would need to be applied repeatedly, at great cost, and may also leave residues in the environment.
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The CSW ProTeens aim to design a synthetic biology solution to this problem with a nonpathogenic strain of E. coli able to produce and secrete RiAFP (Rhagium inquisitor Antifreeze Protein) using a part that the Yale ‘11 iGEM team developed. RiAFP is an insect antifreeze protein from Rhagium inquisitor and the most efficient AFP known. RiAFP, unlike synthetic chemicals, should not be harmful if ingested. We are not sure yet whether this method is effective on an industrial scale and will need to test this. We wish to control the population of the E.coli by designing a kill switch mechanism that will prevent excessive growth as well as use a type 1 secretion system designed by the Utah State University ‘09 iGEM team to transport the protein directly to the extracellular space.
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===Notebook===
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<img src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="500">
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<h3> Solution</h3>
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<p> The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. <a href="https://2014hs.igem.org/Team:CSWProteens/project">Read more about our project.</a></p>
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===Results/Conclusions===
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===Safety===
 
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
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===Attributions===
 
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===Human Practices===
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===Fun!===
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<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><img src="https://static.igem.org/mediawiki/2014hs/7/7a/Tumblr_myr5o6C8Fj1t12eweo2_1280.jpg" width="500">
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<h3> Follow our work in the lab.</h3></a>
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<h3>Human Practices</h3>
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<p> Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.</p>
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Latest revision as of 02:14, 21 June 2014

Welcome!
We are the CSW Proteens!
Scroll down to find more about our project!

PlantiFreeze

Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.

Solution

The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. Read more about our project.

Follow our work in the lab.

Human Practices

Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.

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