Team:CSWProteens

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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<center><a href="https://2014hs.igem.org/Team:CSWProteens/project"><img alt="" class="auto-style11" height="200" src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="200" /><img src="https://static.igem.org/mediawiki/2014hs/7/7e/CSW_Banner.png" width="500">
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<ul id="nav">
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<li><a href="https://2014hs.igem.org/Team:CSWProteens"><b>Home</b></a></li>
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  <li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/team"><b>Team</b></a>
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    <ul>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/team">Members</a></li>
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        <li><a href="http://www.csw.org/">The Cambridge School of Weston</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/advisors">Advisors</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/sponsors">Sponsors</a></li>
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      <li><a href="https://2014hs.igem.org/Team:CSWProteens/Attributions">Attributions</a></li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/project"><b>Project</b></a>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/frostprotection">Frost Protection</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project">Project Description</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/Methods">Methods</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/results">Results</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/discussion">Discussion</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/safety">Safety</a></li>
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</ul>
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<li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><b>Notebook</b></a>
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<ul>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/">Lab Journal</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/protocols">Protocols</a></li>
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<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/parts">Parts</a></li>
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                                <li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/references">References</a></li>
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<a href="https://2014hs.igem.org/Team:CSWProteens/humanpractices"><b>Outreach</b></a>
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<a href="https://2014hs.igem.org/Team:CSWProteens/contact"><b>Contact Us</b></a>
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<li>sponsor information</li>
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<li><a href="http://csw-igem.tumblr.com">Tumblr</a></li>
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<li><a href="http://www.facebook.com/cswigem">Facebook</a></li>
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                                <li><a href="mailto:hgoldsweig@csw.org?subject=iGEM">Email</a></li>
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<h3><p>Welcome!<br> We are the CSW Proteens! <br>Scroll down to find more about our project! </center></h3>
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|align="center"|[[Team:CSWProteens | Team CSWProteens]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=CSWProteens Official Team Profile]
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<h3>PlantiFreeze</h3>
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<p>Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.</p>
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===Team===
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The Cambridge School of Weston is located in Weston instead of Cambridge, Massachusetts in America. It is a progressive, co-educational, day and boarding school for students in grades 9 to 12 and post-graduate. Since the school's inception in 1886, it has been at the forefront of educational innovation.
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Our teachers are passionate. And we are bold, thoughtful, and some of the most interesting people you’ll ever meet. We are The Cambridge School of Weston, CSW Proteens. We will rock the world upside down!
 
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The team consists of 14 intelligent students who stand strong with the best instructors on the earth, Howard G. Goldswag and Melodie Knowlton. Oh, not gold swag but Goldsweig. It must have meant the same. The team is led by Joey Booties, sorry, Joseph Boots-Ebenfield actually, a courageous young lad who started the iGem team for the school from zero to infinity. The team members, Aiden Kolodziej, Jenny Woo, Matthew Waters, Ellie Wolf, Nate Williams, Joey Boots-Ebenfield, Noa Machover, Ben Klebe, Summer Ardell, Claire Kerper, Thomas Schaffner, Micah Rickles-Young, and Liam Kaufer. As united as the round table knights (if you ignore Lancelot) and as smart as Albert Einstein. Ah! And please don't forget the honored webpage editor Yixuan Liu. The editor was "honored" because he was too lazy to do anything. (Just kidding) :)
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===Project===
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Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of tissue.  Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would need to be applied repeatedly, at great cost, and may also leave residues in the environment.
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The CSW ProTeens aim to design a synthetic biology solution to this problem with a nonpathogenic strain of E. coli able to produce and secrete RiAFP (Rhagium inquisitor Antifreeze Protein) using a part that the Yale ‘11 iGEM team developed. RiAFP is an insect antifreeze protein from Rhagium inquisitor and the most efficient AFP known. RiAFP, unlike synthetic chemicals, should not be harmful if ingested. We are not sure yet whether this method is effective on an industrial scale and will need to test this. We wish to control the population of the E.coli by designing a kill switch mechanism that will prevent excessive growth as well as use a type 1 secretion system designed by the Utah State University ‘09 iGEM team to transport the protein directly to the extracellular space.
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===Notebook===
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Show us how you spent your days.
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<img src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="500">
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<h3> Solution</h3>
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<p> The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. <a href="https://2014hs.igem.org/Team:CSWProteens/project">Read more about our project.</a></p>
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===Results/Conclusions===
 
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What did you achieve over the course of your semester?
 
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===Safety===
 
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Safety is always an important thing for our team. When doing labs, at least one of our instructors is always with us.  The lab is well equipped to ensure every team members' safety. We have thick gloves specially designed for preventing things from burning our hands. Goggles to prevent bad things spitting into our eyes. Rubber gloves ensure the sanity and our health. We also have a lab shower and an eye washer that will save our lives if we get into trouble with some dangerous chemicals. Moreover, we all have insurance so we won't pay out of our pockets if something bad happens ;)
 
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===Attributions===
 
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===Human Practices===
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Here it is, one of the biggest humanity issue: NO ENOUGH FOOD FOR EVERYONE. Nowadays, as the global population grows continuously, the demand for food is rising. Many good and bad were done to expand agricultural production. Burning down forests and mountains, genetically modify crops and so on, however, expanding the production is the only thing people have to work on. There is a huge annual lost of crops all over the world caused by natural disasters. Frost is one of the big parts. Our project will provide a cheap and doable way of continuously preventing frost to damage the crops for a long period of time. This means our synthetic biology method is easy to apply and effective. This will contribute to the world's agricultural production and help all Human beings to live in a better world.
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===Fun!===
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<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><img src="https://static.igem.org/mediawiki/2014hs/7/7a/Tumblr_myr5o6C8Fj1t12eweo2_1280.jpg" width="500">
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<h3> Follow our work in the lab.</h3></a>
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<p> Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.</p>
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Latest revision as of 02:14, 21 June 2014

Welcome!
We are the CSW Proteens!
Scroll down to find more about our project!

PlantiFreeze

Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.

Solution

The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. Read more about our project.

Follow our work in the lab.

Human Practices

Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.

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