Team:FHS Frederick MD

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{{:Team:FHS_Frederick_MD/Header}}
{{:Team:FHS_Frederick_MD/Header}}
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=Overview=
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===Team===
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[[File:Tshirts.jpg|right|500px]]
[[File:Tshirts.jpg|right|500px]]
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We are interested in creating a microbial fuel cell that utilizes anaerobic bacteria to produce electricity.  In order to optimize the growth conditions in the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth.  We plan to implement an oxygen-sensitive promoter to induce expression of the glowing gene.  This should ensure that bacteria only grow under anaerobic conditions.  This would lead to the creation of a genetic construct that can be deposited back into the “toolbox” parts repository for iGEM.
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We are interested in creating a [[Team:FHS_Frederick_MD/Microbial_Fuel_Cells|microbial fuel cell]] that utilizes the facultative anaerobic bacteria, ''Shewanella oneidensis'', to produce [[Team:FHS_Frederick_MD/Renewable_Energy|renewable energy]] and [[Team:FHS_Frederick_MD/Clean_Water|clean water]].   
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===Goals===
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===Gene Design===
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(Kyle and Jonathon, briefly summarize how NirB and LOV work together.)
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===Methods===
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(This is Dillon's domain.)
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====3A Assembly====
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In order to optimize the growth conditions in the anaerobic chamber of the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth under different conditions. 
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We used the 3A, or 3 antibody, assembly kit in order to transform E.coli with two genes, the LOV gene and the NirB gene.  These genes  will allow for further work with Schwenella bacteria in the anaerobic microbial fuel cell. We then used the mini-prep components of the kit to purify our plasmid. We verified the plasmid's presence through electrophoresis and further sequence analysis.
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We plan to accomplish this using the [[Team:FHS_Frederick_MD/NirB_Promoter|NirB oxygen-sensitive promoter]] to induce expression of the glowing gene only when oxygen is scarce.  Furthermore, we must engineer a fluorescent [[Team:FHS_Frederick_MD/LOV_Domain|LOV domain]], which is optimized for expression in ''S. oneidensis'' and capable of fluorescence under anaerobic conditions.   
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===Results/Conclusions===
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This genetic construct will help us ensure that the bacteria are actually growing under anaerobic conditions.  This would lead to the creation of a BioBrick that can be deposited back into the “toolbox” parts repository for future iGEM teams.
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What did you achieve over the course of your semester?
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{{:Team:FHS_Frederick_MD/Footer}}

Latest revision as of 01:43, 21 June 2014

FHS Logo.png


Overview

Tshirts.jpg

We are interested in creating a microbial fuel cell that utilizes the facultative anaerobic bacteria, Shewanella oneidensis, to produce renewable energy and clean water.

In order to optimize the growth conditions in the anaerobic chamber of the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth under different conditions.

We plan to accomplish this using the NirB oxygen-sensitive promoter to induce expression of the glowing gene only when oxygen is scarce. Furthermore, we must engineer a fluorescent LOV domain, which is optimized for expression in S. oneidensis and capable of fluorescence under anaerobic conditions.

This genetic construct will help us ensure that the bacteria are actually growing under anaerobic conditions. This would lead to the creation of a BioBrick that can be deposited back into the “toolbox” parts repository for future iGEM teams.

IGEM-logo.png


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