Team:Beijing HDFLS High/Project

From 2014hs.igem.org

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  <title>太空机器人 - www.itccc.org.cn | 青少年国际竞赛与交流中心</title>
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  <meta name="keywords" content="科学夏令营 科学探索 机器人比赛 艺术 国际竞赛 国际交流">
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The protective melittin generator;  
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  <meta name="description" content="青少年国际竞赛与交流中心致力于引入国外优质的教育资源，以提升中国青少年的科学素养，国际视野，培养他们的健全人格。">
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The UV-responsing tandem generators for a wintergreen odor and protective melittin.
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=ABSTRACT=
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The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin  can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as  blue light and UV.
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[[File:The_protective_melittin_generator.png|center|400px]]
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We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes  two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and  the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.
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[[File:Tandem Generators.png|center|800px]]
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=BACKGROUND=
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===Blue light from computer induces eye retina producing free radical===
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The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.
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Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle. The blue light induce that retinal produce free radicals. These radicals can lead to retinal pigment epithelial cell death. Then some light-sensitive cells lacks of nutrient, which results in visual impairment.
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{
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[[File:Blue light.png|center|600px]]
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===Melittin has funcition of scavenging free radical===
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The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.
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[[File:bee3.png|center|700px]]
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Melittin is the main component (50~55%) of bee venom,  which has multiple functions.
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[[File:Melittin3.png|center|600px]]
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[[File:Melittin function.png|center|600px]]
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=PARTS=
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=I: Parts of Protective Melittin Generators.=
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[[image:part.png|center|400px]]
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==Part A: Rec A (SOS) promoter==
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Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in registry part plate.
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[[Image: Part A.jpg|center|400px]]
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==Part B: Fusion melittin ==
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{
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We synthesized fusion melittin gene with enterokinase digestion site. Melittin has the function of scavenging free radicals.
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[[Image:melittin.jpg|center|700px]]
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=II: Parts of Tandem Generators.=
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==Part A: Wintergreen Odor Generator==
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The generator was constructed last year, and  can produce wintergreen odor under extensive UV radiation
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==Part B: Protective Melittin Genertor==
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The generator is constructed this year, and can produce protective melittin under extensive UV radiation
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=METHODS=
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===METHOD I: The Protective Melittin Generator===
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[[Image:method I.jpg|center|800px]]
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===METHOD II: The Tandem Generators===
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We construct tandem Generators, using similar method with protective melittin generator
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=RESULTS=
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===Result I:The Protective Melittin Generator===
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=====Plates=====
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[[Image:Result Plate 2.jpg|center|800px]]
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=====Electrophoresis Map after Restriction Digest=====
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[[Image:Result Map4.jpg|center|900px]]
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=====DNA Sequencing and Submitting Part=====
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http://parts.igem.org/Part:BBa_K1283000
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===Result II:Tandem Generators===
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[[Image:Result IIPlate .jpg|center|400px]]
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=====Electrophoresis Map after Restriction Digest=====
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[[Image:Result electrophoresis .jpg|center|600px]]
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===== DNA Sequencing and Submitting Part=====
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http://parts.igem.org/Part:BBa_K1283002
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===Step1 Transformation and Miniprep===
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We get Part A plasmid from registry, and construct Part B plasmid.
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===Step2 Digestion===
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===Step3 Ligation===
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===Step4 Tansformation, Miniprep and Digestion again===
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[[File:step4.png|center|600px]]
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===Step5 Sequencing===
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<DIV class=nav
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style="MARGIN-TOP: 332px; FONT-SIZE: 12px; FLOAT: right; WIDTH: 200px; COLOR: #fff; LINE-HEIGHT: 30px; HEIGHT: 30px; BACKGROUND-COLOR: #ff6c00; TEXT-ALIGN: center"><A
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href="../../index.html"><STRONG>返回中心首页</STRONG></A>
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| <A href="../../camps.html"><STRONG>所有特训营</STRONG></A></DIV></DIV>
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<div style="width:980px; margin-top:22px;">
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<!--介绍开始-->
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<div style=" height:390px; background:url(images/stf_02.jpg);">
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<div style="width:310px;  padding-top:18px; padding-left:40px; float:left;">
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<p style="font-size:14px; line-height:24px;"><strong style="color:#F00;">主办方：</strong><br>
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中国教育国际交流协会青少年国竞赛与交流中心<br>
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美国太空与火箭中心<br>
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<strong style="color:#F00;">时 间：</strong><br>
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2014年8月6日—8月19日<br>
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<strong style="color:#F00;">招生对象：</strong><br>
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9-14岁青少年，喜爱机器人学习与实践<br>
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9-11岁为一个组别，12-14岁为一个组别<br>
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<strong style="color:#F00;">咨询热线：</strong><br>
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010-66537227，15001081324  <br>
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<strong style="color:#F00;">咨询邮箱：</strong><br>
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zhaoyang@itccc.org.cn<br>
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  </p>
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<div style="width:500px; padding-top:30px; padding-left:60px; float:left;">
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  <p style="font-size:20px; line-height:30px;">
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美国太空火箭中心是由美国宇航局(NASA)所创立，这里是一个关于太空、工程、物理、天文和机器人的综合教育基地。欢迎同学们来到这里，加入实现太空梦想的行列。自从1982年中心建立，到今天为止，已经有超过500,000人在太空火箭中心中参加学习。你还可以在这里接受正式的培训，优秀的学生甚至可以得到太空中心出具的正式大学推荐信。太空营全部培训设备为美国航天员所用模拟设备，这里也是世界上最大的太空和火箭博物馆，为学生提供太空旅行的体验，提高学习高科技的兴趣。
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  </p>
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</div></div>
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<!--介绍结束-->
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<div class="xinzeng">
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<img src="./ts.jpg">
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<strong>特色一：由美国宇航局（NASA）创立</strong><br>
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这里是由NASA设立的世界最大的一个综合教育基地，你将在最专业的设备和最有经验的教师的帮助下，学习用机器人探索宇宙的奥秘，实现从小就怀有的太空梦想。<br />
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<div class="mokuai-2">
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<div class="mokuai-zuo"><img src="./ts_02.jpg"></div>
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<div class="mokuai-you"><strong>特色二：同来自各国的小伙伴共同学习</strong><br>
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团队合作是太空特训营的特色之一，你将与来自世界各地的小伙伴一起组成小组，共同克服困难，完成任务。<br />
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<div class="mokuai-zuo"><img src="./ts_03.jpg"></div>
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<div class="mokuai-you"><strong>特色三：模拟宇航员的生活</strong><br>
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学生们将身着宇航服，住宿太空舱，锻炼独立生活能力，团队互助精神。<br>
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教官通宵在宿舍外值班守护，营地配备24小时诊断服务。<br>
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</div>
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<div class="mokuai-zuo"><img src="./ts_04.jpg"></div>
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<div class="mokuai-you"><strong>特色四：获得证书和推荐信
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</strong><br>
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参加太空特训营的学员都将获得正式证书，优秀学员还将得到额外的奖励。<br />
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<!--行程开始-->
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<p style="font-size:24px; font-weight:bold; text-align:center;">行程安排</p>
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<p><img src="./10.jpg"></p>
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<div style="width:900px;line-height:26px; margin-left:30px;">
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<strong style="color:#F00; font-size:16px;">Day1</strong><br>
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北京出发，到达纽约。<br />
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</div>
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<div style="width:920px; height:300px; margin-left:30px; margin-top:20px; background:#eeeeee;">
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<img src="./0-1.jpg" width="405" height="269"><br>
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<div style="width:405px; height:269px; float:left; margin-left:30px; padding-top:15px;">
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<div style=" height:150px; width:950px; margin-left:15px; margin-top:10px; float:left;">
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<div style="width:450px; height:140px; padding-left:20px; background:#FFF;  float:left;">
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00;font-weight:bold;padding-top:10px;">Day2</p>
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<p style="font-size:14px;line-height:24px;width:440px;"><strong>全天：</strong>参观时代广场、第五大道、自由女神像； 参观联合国总部、华尔街。<br />
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<div style="width:470px; height:140px; float:left; background:#FFF;  margin-left:10px;"> 
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00;padding-left:20px; font-weight:bold;padding-top:10px;">Day3</p>
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<p style="font-size:14px;padding-left:20px; line-height:24px;width:440px;"><strong>全天：</strong>前往波士顿，参观世界名校哈佛大学、麻省理工大学。<br>
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晚上返回纽约。<br  />
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00;font-weight:bold;padding-top:10px;">Day4</p>
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<p style="font-size:14px;line-height:24px;width:440px;"><strong>全天：</strong>参观纽约大都会博物馆、中央公园。<br />
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<div style="width:470px; height:140px; float:left; background:#FFF;  margin-left:10px;"> 
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00;padding-left:20px; font-weight:bold;padding-top:10px;">Day5</p>
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<p style="font-size:14px;padding-left:20px; line-height:24px;width:440px;"><strong>上午：</strong>前往太空火箭中心。
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<strong>下午：</strong>太空机器人学习体验营欢迎礼。<br>
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<strong>晚间：</strong>课程准备。<br  />
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<div style="width:950px; height:180px; float:left; margin-left:15px; background:#FFF;">
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<div style="width:900px; height:150px; float:left; margin-left:40px; margin-top:15px; display:inline;">
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<div style="width:200px; height:150px; float:left;"><img src="./1-1.jpg"></div>
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<div style="width:200px; height:150px; float:left; margin-left:20px;"><img src="./1-2.jpg"></div>
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<div style="width:200px; height:150px; float:left; margin-left:20px;"><img src="./1-3.jpg"></div>
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<div style="width:200px; height:150px; float:left; margin-left:20px;"><img src="./1-4.jpg"></div>
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<div style=" height:auto; width:980px; float:left;background:#efefef;">
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<div style=" height:260px; width:950px; margin-left:15px; margin-top:10px;">
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  <div style="width:930px; height:250px; padding-left:20px; background:#FFF;  float:left;">
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00; font-weight:bold;padding-top:10px;">Day6 - Day9&nbsp;&nbsp;宇航科学家的生活</p>
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<p style="font-size:14px; line-height:24px;"><strong>太空机器人特训营中充实的一天</strong><br />
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<table>
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<td width=350px>
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7:00am—8:30am&nbsp;&nbsp;起床，早餐
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<td>
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8:30am—10:00am&nbsp;&nbsp;太空机器人知识学习
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10:00am—11:30am&nbsp;&nbsp;团队建设活动 
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11:30am—noon&nbsp;&nbsp;宇航餐厅午餐
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12:30pm—1:30pm&nbsp;&nbsp;宇航员模拟训练 
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</td>
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1:30pm—3:00pm&nbsp;&nbsp;火箭建造原理学习
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3:00pm—4:00pm&nbsp;&nbsp;3D太空展示
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</td>
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4:00pm—5:00pm&nbsp;&nbsp;机器人模拟任务训练
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5:30pm—6:00pm&nbsp;&nbsp;晚餐
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6:30pm—7:30pm&nbsp;&nbsp;回顾太空火箭中心的发展史
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7:30pm—8:30pm&nbsp;&nbsp;工程设计实践
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8:30pm—10:00pm&nbsp;&nbsp;回到休息舱，准备休息
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<div style="width:200px; height:150px; float:left;"><img src="./3-1.jpg"></div>
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00; font-weight:bold;padding-top:10px;">Day10</p>
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<p style="font-size:14px; line-height:24px;">机器人实战比拼，展示学习成果，评出获胜团队，颁发奖品和结业证书。<br />
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<div style="width:980px; height:300px; float:left; background:#eee;">
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<div style="width:423px; height:260px; float:left;"><img src="./2-1.jpg"></div>
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<div style="width:387px; height:260px; float:left; margin-left:20px;"><img src="./2-2.jpg"></div>
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<p style="font-size:18px; font-family:&#39;黑体&#39;; color:#F00; font-weight:bold;padding-top:10px;">Day13</p>
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<p style="font-size:14px; line-height:24px; width:450px;">返程归国。<br>
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<p style="font-size:14px;padding-left:20px; line-height:24px;width:430px;">抵达北京。
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Latest revision as of 12:45, 20 June 2014

Header wiki.png

Beijing HDFLS iGEM 2013

 



Contents

TITLE

The protective melittin generator; The UV-responsing tandem generators for a wintergreen odor and protective melittin.

ABSTRACT

The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.

The protective melittin generator.png

We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.

Tandem Generators.png

BACKGROUND

Blue light from computer induces eye retina producing free radical

The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.

2000px-EM spectrum.png

Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle. The blue light induce that retinal produce free radicals. These radicals can lead to retinal pigment epithelial cell death. Then some light-sensitive cells lacks of nutrient, which results in visual impairment.

Blue light.png

Melittin has funcition of scavenging free radical

The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.

Bee3.png

Melittin is the main component (50~55%) of bee venom, which has multiple functions.

Melittin3.png


Melittin function.png

PARTS

I: Parts of Protective Melittin Generators.

Part.png

Part A: Rec A (SOS) promoter

Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in registry part plate.

Part A.jpg

Part B: Fusion melittin

We synthesized fusion melittin gene with enterokinase digestion site. Melittin has the function of scavenging free radicals.

Melittin.jpg

II: Parts of Tandem Generators.

Part A: Wintergreen Odor Generator

The generator was constructed last year, and can produce wintergreen odor under extensive UV radiation

Part B: Protective Melittin Genertor

The generator is constructed this year, and can produce protective melittin under extensive UV radiation

METHODS

METHOD I: The Protective Melittin Generator

Method I.jpg

METHOD II: The Tandem Generators

We construct tandem Generators, using similar method with protective melittin generator

RESULTS

Result I:The Protective Melittin Generator

Plates
Result Plate 2.jpg
Electrophoresis Map after Restriction Digest
Result Map4.jpg
DNA Sequencing and Submitting Part

http://parts.igem.org/Part:BBa_K1283000

1283000.jpg

Result II:Tandem Generators

Plates
Result IIPlate .jpg
Electrophoresis Map after Restriction Digest
Result electrophoresis .jpg
DNA Sequencing and Submitting Part

http://parts.igem.org/Part:BBa_K1283002

1283002.jpg

STEPS

Step1 Transformation and Miniprep

We get Part A plasmid from registry, and construct Part B plasmid.

Step1.png

Step2 Digestion

Step2.png

Step3 Ligation

Step3.png

Step4 Tansformation, Miniprep and Digestion again

Step4.png

Step5 Sequencing

Retrieved from "http://2014hs.igem.org/Team:Beijing_HDFLS_High/Project"