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Team:PEA Exeter NH/Notebook Raw
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Samples from Miniprep</br> | Samples from Miniprep</br> | ||
Results: DNA Concentrations</br> | Results: DNA Concentrations</br> | ||
| - | NAP1:</br> | + | NAP1:26.25 ng/ul</br> |
| - | NAP2:</br> | + | NAP2:33.50 ng/ul</br> |
| - | CAT1:</br> | + | CAT1:39.50 ng/ul</br> |
| - | CAT2:</br> | + | CAT2:21.00 ng/ul</br> |
| - | SOS1:</br> | + | SOS1:29.75 ng/ul</br> |
| - | SOS2 (from mini prep on 5.21.14):</br> | + | SOS2 (from mini prep on 5.21.14):30.50 ng/ul</br> |
| - | BLUE1:</br> | + | BLUE1:70.25 ng/ul</br> |
| - | BLUE2 | + | BLUE2:20.50 ng/ul</br> |
</br> | </br> | ||
</br> | </br> | ||
| Line 166: | Line 166: | ||
</br> | </br> | ||
Loading Order of Gel:</br> | Loading Order of Gel:</br> | ||
| - | 100 base pair ladder | + | 100 base pair ladder</br> |
| - | Ampicillin Backbone Digest 1 | + | Ampicillin Backbone Digest 1</br> |
| - | Ampicillin Backbone Digest 2 | + | Ampicillin Backbone Digest 2</br> |
| - | Kanamycin Backbone Digest | + | Kanamycin Backbone Digest</br> |
| - | NAP1 | + | NAP1</br> |
| - | NAP2 | + | NAP2</br> |
| - | CAT1 | + | CAT1</br> |
| - | CAT2 | + | CAT2</br> |
| - | Blue | + | Blue</br> |
| - | SOS | + | SOS</br> |
</br> | </br> | ||
</br> | </br> | ||
Revision as of 05:33, 20 June 2014
Starting in the 2013 Winter term, our team learned laboratory techniques and protocols. We began research on the effects of fracking and the chemical characteristics of toxic water contaminants: Catechol, naphthalene, and lead. Over the spring semester of 2014, six full time team members were working tirelessly on the project. Here, we present a summary of our daily progress and experiments.
GROWING CELL CULTURES
Names: Christina S, Janice C, Melody N
Date: April 29th, 2014
Species: E. Coli
Part number: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009
Results: TBA
PICKING CELL CULTURES
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 30th, 2014
Species: E. Coli
Part number and plasmid: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009, pSB1C3
Results: TBA
RESTRICTION DIGEST
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: May 18th, 2014
Species: E. Coli
Restricted: BBa_J61051, BBa_K118021
Results: TBA
Incubate 20 hours at 37°C
GROWING CELL CULTURE
Names: Christina S
Date: May 20th, 2014
Species: E. Coli
PREPARATION OF ANTIBIOTIC SOLUTION
Names: Christina S
Date: May 20th, 2014
Antibiotic: Chloramphenicol
MINIPREP
Names: Alex S, Janice C, Melody N
Date: May 21st, 2014
Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3
Species: E. Coli
Results: Worked Well
LIGATION
Names: Charis E, Reginald L, Christina S, Cat O
Date: May 21st, 2014
Species: E. Coli
Results: TBA
Tube 1- BBa_J61051_BBa_K118021_pSB1C3
MINIPREP:
Names: Christina S.
Date: May 22nd, 2014
Cultures for Miniprep: BBa_K518010_pSB1C3 (SOS), BBa_J61051_pSB1C3 (NAP), BBa_K118021 _pSB1C3 (CAT), BBa_K592009_pSB1C3 (BLUE) [samples prepped in duplicate]
Results: TBA
MEASURING DNA CONCENTRATION FROM MINIPREP
Names: Alex S, Christina S
Date: May 23rd, 2014
Samples from Miniprep
Results: DNA Concentrations
NAP1:26.25 ng/ul
NAP2:33.50 ng/ul
CAT1:39.50 ng/ul
CAT2:21.00 ng/ul
SOS1:29.75 ng/ul
SOS2 (from mini prep on 5.21.14):30.50 ng/ul
BLUE1:70.25 ng/ul
BLUE2:20.50 ng/ul
RESTRICITION DIGEST
Names: Christina S, Reginald L., Alex S.
Date: May 23rd, 2014
Samples from Miniprep
GEL ELECTROPHORESIS ON DIGEST PRODUCT
Names: Christina S
Date: May 25th, 2014
Samples from restriction digest
Loading Order of Gel: 100 base pair ladder Ampicillin Backbone Digest 1 Ampicillin Backbone Digest 2 Kanamycin Backbone Digest NAP1 NAP2 CAT1 CAT2 Blue SOS LIGATION Names: Alex S, Cat O, Connie C, Reginald L, Janice C Date: May 25th, 2014 Samples from restriction digest MAKING AMPICILLIN/KANAMYCIN PLATES Name: Christina S Date: May 26th, 2014 TRANSFORMATION Names: Cat O, Christina S Date: May27th, 2014 Samples from Ligation GROWING UP CELLS Names: Christina S Date: May 30th, 2014 Species: E. Coli Results: TBA SETTING VARIABLE ENVIRONMENTS FOR CELLS Names: Christina S, Cat O Date: June 1st, 2014 Species: E. Coli Results: TBA EXPERIMENTATION Date: June 2nd to 5th, 2014 Species: E. Coli Results: TBA
Experiment Result to Be Shown At Jamboree!
