Team:CIDEB-UANL Mexico/labwork results

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<p><b><h2>Viability in salt (experiment 1)</h2></b></p>
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<p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p>
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<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Rojas_NhaS_experiment_1.jpg"
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align=center hspace=12 alt="IMG_0317"></p>
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<p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center>
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<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Blancas_NhaS_experimen_1.jpg"
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<p><center><b>Image 2. </b>All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.</p></center>
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<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Control_Expriment_1_NhaS_.jpg"
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align=center hspace=12 alt="IMG_0317"></p>
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<p><center><b>Image 3. </b>All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.</p></center>
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<p>All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.</p>
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<p>None of the control group lived in any concentration of salt.</p>
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<center><p><img width=262 height=403 src="https://2014hs.igem.org/File:Experiment_1_Maximum_concentration_of_salt..jpg"
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<p><center><b>Image 4. </b>Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria. </p></center>
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Revision as of 03:46, 15 June 2014

Methods Notebook Results Interpretations Conclusions Future Plans
iGEM CIDEB 2014 - Project

Results

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

IMG_0317

Image 1. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 2. All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 3. All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.

All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.

None of the control group lived in any concentration of salt.


IMG_0317

Image 4. Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.


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