Team:AUC TURKEY

From 2014hs.igem.org

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===Project Description===
===Project Description===
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This year we are working on the project 'BacteCooler 2.0'.
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Our project this year will be the continuation of our last year's project. In 'BactoCooler 2.0' we shall make major improvements to the project itself to allow it to reach the state in which it can be effectively applied in real life as a product.
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In this project our aim is improve our last year project BacteCooler. Last year, in the beginning of the BacteCooler project we decided to use our project in real life as a product. This year we analyzed our project in terms of adaption BacteCooler to reality and found three main problems. Our solutions to these problems are;
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After the analysis of the functions of the project last year, we discovered three main problems. The problems with their solutions are as follows:
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1) For safety of our project we should develop Kill-switch mechanisms. This mechanism activate on case of our bacteria contact with air. So we are working on the promoters that sensible to molecules in the air.
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1)There are safety issues with BactoCooler being released into the environment and specifically into the air.
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2) In our BacteCooler project our enzyme Urease catalyzes the urea in the environment. After this catalyzation reaction NH3 come out and accumulate in the environment. This accumulation of NH3 causes the increase of pH and alkalization of environment. After this increase of pH our bacteria will be dead. For the solving this problem we designed a circuit that activated with pH sensible promoter and then produce an acidic buffer protein that buffering this pH increase.
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Through installing Kill-Switch mechanisms designed to activate on air contact, we can remove concerns of contamination.
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3) Final and the biggest problem is over-night incubation time problem. For the solving this problem we should develop a system that secrete our urease enzyme quickly after the increase of temperature. We examined some of the iGEM projects and find some solutions that suit our project.  
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2)BactoCooler synthesizes the urease enzyme which catalyzes the breakdown of the urea in the environment eventually leading to the accumulation of ammonia. Ammonia, being a basic compound, raises the pH of the environment after accumulating and causes alkalization. The highly basic environment results in the death of BactoCooler.  
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This year for get a more efficient and exact results in our project we started to learn protein expressions experiment like Western-Blot, SDS-Page and ELISA. Also we working on modelling for get more information about how our project will work on practice.
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To counteract the issue, a pH sensitive system which will detect the alkaline conditions will allow the secretion of an acidic buffer protein will be integrated in 'BactoCooler 2.0'.
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Finally we can say that in this iGEM HS 2014 adventure, we will try to solve these three problems with successful and much fun.
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3)The time required for over-night incubation is much too long making immediate effect impossible to reach. Aside from the long term effect, due to the nature of our project,  we the switch system regulated by the RNA Thermometer cannot be actively used if a short term response is not achieved.
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We have conducted research to find possible solutions on the matter and found some possible solutions that suit our project from the work of previous iGEM projects. Different protein expression experiments such as the Western-Blot, SDS-Page and ELISA can serve our purpose.
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Alongside the application of the possible solutions to 'BactoCooler 2.0', we are planning to do modelling to inspect the real-life application of our project.
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As AUC_TURKEY we would like to state our wishes of an enjoyable and enthusiastic year of iGEM to every team and iGEM HQ.
===Notebook===
===Notebook===

Revision as of 19:03, 12 March 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:AUC_TURKEY/Our_Pets



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
File:AUC TURKEY logo.png
200px

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:AUC TURKEY team.png
Your team picture
Team AUC_TURKEY


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project Description

Our project this year will be the continuation of our last year's project. In 'BactoCooler 2.0' we shall make major improvements to the project itself to allow it to reach the state in which it can be effectively applied in real life as a product. After the analysis of the functions of the project last year, we discovered three main problems. The problems with their solutions are as follows: 1)There are safety issues with BactoCooler being released into the environment and specifically into the air. 

Through installing Kill-Switch mechanisms designed to activate on air contact, we can remove concerns of contamination.

2)BactoCooler synthesizes the urease enzyme which catalyzes the breakdown of the urea in the environment eventually leading to the accumulation of ammonia. Ammonia, being a basic compound, raises the pH of the environment after accumulating and causes alkalization. The highly basic environment results in the death of BactoCooler. To counteract the issue, a pH sensitive system which will detect the alkaline conditions will allow the secretion of an acidic buffer protein will be integrated in 'BactoCooler 2.0'. 3)The time required for over-night incubation is much too long making immediate effect impossible to reach. Aside from the long term effect, due to the nature of our project, we the switch system regulated by the RNA Thermometer cannot be actively used if a short term response is not achieved. We have conducted research to find possible solutions on the matter and found some possible solutions that suit our project from the work of previous iGEM projects. Different protein expression experiments such as the Western-Blot, SDS-Page and ELISA can serve our purpose. Alongside the application of the possible solutions to 'BactoCooler 2.0', we are planning to do modelling to inspect the real-life application of our project. As AUC_TURKEY we would like to state our wishes of an enjoyable and enthusiastic year of iGEM to every team and iGEM HQ.

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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