Team:Penn Mishawaka IN

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{|align="justify"
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
|[[Image:Penn_Mishawaka_IN_logo.png|200px|right|frame]]
|-
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
|[[Image:Penn_Mishawaka_IN_team.png|right|frame|Your team picture]]
|-
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|align="center"|[[Team:Penn_Mishawaka_IN | Team Penn_Mishawaka_IN]]
|}

<!--- Team Information Link --->

{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Penn_Mishawaka_IN Official Team Profile]
|}

===Team===
Tell us about your team, your school!

===Project===

== The presence of arsenic in drinking water provides a complicated obstacle for 

developing countries. Some 100 million people are affected by arsenic poisoning 

worldwide, and the problem is exasperated by the unpredictable patterns of contamination. 

This necessitates frequent and comprehensive testing of well water; Bangladesh alone has 

10 million water wells that must be checked twice a year. Effective arsenic tests have been 

created in the past, yet they often require expensive reagents, advanced technology and 

specialized technicians – not feasible for large-scale operations in the developing world. 

Our team endeavors to create a simple, biologically-based test for arsenic. Using 

existing BioBricks and building off the ideas of previous iGEM projects, we have designed 

two systems that work within E.coli to detect the presence of arsenic in a water supply and 

generate a strong, noticeable response. The first links an arsR promoter (BBa_J33201) 

turned on in the presence of arsenic to a firefly luciferase reporter gene (BBa_K325909) 

that emits a bioluminescent glow. Additionally in the system is a second luciferase reporter 

gene, (BBa_K325209) attached to a constitutive gene that will constantly emit 

bioluminescence of a different color to serve as an indicator of normal cell function. Ideally, 

the end result would be cells that will glow in a dark room, change colors (yellow to green) 

in the presence of arsenic, and stop glowing entirely if they are killed by arsenic or any 

other toxin. 

Additionally, we are in the process of designing a cascade sequence to detect and 

react to the presence of arsenic. A cascade sequence provides the benefit of 

customizability: we can control the reaction at many levels, both by ensuring when the 

sequence is turned on or off and by controlling the amount of output created by the system 

at different levels of arsenic.
 ==

== [[Headline text]] ==

===Notebook===
Show us how you spent your days.


===Results/Conclusions===
What did you achieve over the course of your semester?


===Safety===
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab? 


===Attributions===
Who worked on what?


===Human Practices===
What impact does/will your project have on the public?  


===Fun!===
What was your favorite team snack?? Have a picture of your team mascot? 



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