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All of these steps can be seen in the 3A Assembly kit protocols | All of these steps can be seen in the 3A Assembly kit protocols | ||
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On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.
On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.
The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.
All of these steps can be seen in the 3A Assembly kit protocols
Vik Was Here
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