Team:CSWProteens

From 2014hs.igem.org

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===Project===
===Project===
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What are you working on this semester?
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Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of tissue.  Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly,  and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would need to be applied repeatedly, at great cost, and may also leave residues in the environment.
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The CSW ProTeens aim to design a synthetic biology solution to this problem with a nonpathogenic strain of E. coli able to produce and secrete RiAFP (Rhagium inquisitor Antifreeze Protein) using a part that the Yale ‘11 iGEM team developed. RiAFP is an insect antifreeze protein from Rhagium inquisitor and the most efficient AFP known. RiAFP, unlike synthetic chemicals, should not be harmful if ingested. We are not sure yet whether this method is effective on an industrial scale and will need to test this. We wish to control the population of the E.coli by designing a kill switch mechanism that will prevent excessive growth as well as use a type 1 secretion system designed by the Utah State University ‘09 iGEM team to transport the protein directly to the extracellular space.
===Notebook===
===Notebook===

Revision as of 20:52, 6 March 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:CSWProteens/Our_Pets



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
CSWProteens logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:CSWProteens team.png
Your team picture
Team CSWProteens


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project

Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would need to be applied repeatedly, at great cost, and may also leave residues in the environment. The CSW ProTeens aim to design a synthetic biology solution to this problem with a nonpathogenic strain of E. coli able to produce and secrete RiAFP (Rhagium inquisitor Antifreeze Protein) using a part that the Yale ‘11 iGEM team developed. RiAFP is an insect antifreeze protein from Rhagium inquisitor and the most efficient AFP known. RiAFP, unlike synthetic chemicals, should not be harmful if ingested. We are not sure yet whether this method is effective on an industrial scale and will need to test this. We wish to control the population of the E.coli by designing a kill switch mechanism that will prevent excessive growth as well as use a type 1 secretion system designed by the Utah State University ‘09 iGEM team to transport the protein directly to the extracellular space.

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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