Notebook

On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.

On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.

The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.

All of these steps can be seen in the 3A Assembly kit protocols