Team:CIDEB-UANL Mexico/project resistance
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<p><b>IrrEparts description :</b></p> | <p><b>IrrEparts description :</b></p> | ||
- | <center><p><img width= | + | <center><div> |
- | align=center hspace=12 alt=" | + | <table class=MsoTable15Grid7ColorfulAccent6 border=1 cellspacing=0 |
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+ | mso-border-themetint:153;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <tr style='mso-yfti-irow:-1;mso-yfti-firstrow:yes;mso-yfti-lastfirstrow:yes; | ||
+ | height:15.75pt'> | ||
+ | <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-bottom: | ||
+ | solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: | ||
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+ | background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:517'><b><span lang=ES-MX | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX;mso-bidi-font-style: | ||
+ | italic'>IMAGE</span></b><b style='mso-bidi-font-weight:normal'><span | ||
+ | lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: | ||
+ | "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language: | ||
+ | ES-MX;mso-bidi-font-style:italic'><o:p></o:p></span></b></p> | ||
+ | </td> | ||
+ | <td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom: | ||
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+ | accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: | ||
+ | background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE</span></b><b | ||
+ | style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> | ||
+ | </td> | ||
+ | <td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom: | ||
+ | solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: | ||
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+ | accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: | ||
+ | background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION</span></b><b | ||
+ | style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> | ||
+ | </td> | ||
+ | </tr> | ||
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+ | solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: | ||
+ | 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; | ||
+ | height:60.75pt'> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
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+ | visibility:visible;mso-wrap-style:square;mso-width-percent:0; | ||
+ | mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0; | ||
+ | mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0; | ||
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+ | mso-height-relative:page'> | ||
+ | <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/> | ||
+ | <w:wrap type="square"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=82 height=65 | ||
+ | src="TablasAromaYResistencia_archivos/image002.png" align=left hspace=12 | ||
+ | v:shapes="_x0031__x0020_Imagen"><![endif]><i><span lang=ES-MX | ||
+ | style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade: | ||
+ | 191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:right;line-height:normal;mso-yfti-cnfc:68'> </p></td> | ||
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+ | background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: | ||
+ | 51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:64'><span lang=ES-MX | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p></span></p> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:64'> </p> | ||
+ | </td> | ||
+ | <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: | ||
+ | none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; | ||
+ | mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: | ||
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+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: | ||
+ | solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: | ||
+ | 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; | ||
+ | mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; | ||
+ | mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> | ||
+ | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: | ||
+ | justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In | ||
+ | the specific case of our bacteria, it helps to continuously transcribing the <span | ||
+ | class=SpellE>IrrE</span> gene in order to make bacteria resist high | ||
+ | concentration of salt. </span><span class=SpellE><span lang=ES-MX | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This</span></span><span | ||
+ | lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: | ||
+ | "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language: | ||
+ | ES-MX'> <span class=SpellE>promoter</span> has a <span class=SpellE>length</span> | ||
+ | of 35 <span class=SpellE>pb</span> (Anderson, 2006).<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:1;height:75.0pt'> | ||
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+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: | ||
+ | solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: | ||
+ | 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; | ||
+ | height:75.0pt'> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape | ||
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+ | height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square; | ||
+ | mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; | ||
+ | mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; | ||
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+ | mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> | ||
+ | <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/> | ||
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+ | </v:shape><![endif]--><![if !vml]><img width=72 height=66 | ||
+ | src="TablasAromaYResistencia_archivos/image004.png" align=left hspace=12 | ||
+ | v:shapes="_x0032__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; | ||
+ | color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: | ||
+ | EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:right;line-height:normal;mso-yfti-cnfc:4'> </p> | ||
+ | </td> | ||
+ | <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; | ||
+ | border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: | ||
+ | accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; | ||
+ | mso-border-right-themecolor:accent6;mso-border-right-themetint:153; | ||
+ | mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; | ||
+ | mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; | ||
+ | mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: | ||
+ | solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal'><span style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language: | ||
+ | ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p></span></p> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal'> </p> | ||
+ | </td> | ||
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+ | accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; | ||
+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: | ||
+ | solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: | ||
+ | 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; | ||
+ | mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> | ||
+ | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: | ||
+ | justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; | ||
+ | mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial; | ||
+ | color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This | ||
+ | specific is <span class=SpellE>RBS</span> based on <span class=SpellE>Elowitz</span> | ||
+ | <span class=SpellE>repressilator</span>. It is very common to see in many <span | ||
+ | class=SpellE>iGEM</span> projects.<span style='mso-spacerun:yes'> </span>It | ||
+ | has a length of 12 <span class=SpellE>pb</span>. (Mahajan, <span | ||
+ | class=SpellE>Marinescu</span>, Chow, <span class=SpellE>Wissner</span>-Gross, | ||
+ | & Carr, 2003).<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
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+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: | ||
+ | solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: | ||
+ | 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; | ||
+ | height:90.35pt'> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
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+ | 51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:64'><span | ||
+ | style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p></span></p> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal;mso-yfti-cnfc:64'><span | ||
+ | style='font-size:3.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; | ||
+ | mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ES-MX'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: | ||
+ | none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; | ||
+ | mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: | ||
+ | accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; | ||
+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: | ||
+ | solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: | ||
+ | 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; | ||
+ | mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; | ||
+ | mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> | ||
+ | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: | ||
+ | justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene | ||
+ | that produces <span class=SpellE>IrrE</span>, a substance that changes the | ||
+ | bacteria’s metabolism and allows bacteria to survive to extreme conditions, | ||
+ | some examples could be high UV rays exposition, or high salt concentration | ||
+ | levels in an aquatic environment, oxidative or thermal shock.<span | ||
+ | style='mso-spacerun:yes'> </span>It has a length of 933pb (<span | ||
+ | class=SpellE>Sohrabi</span>, 2012).<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes;height:62.25pt'> | ||
+ | <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; | ||
+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: | ||
+ | none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: | ||
+ | accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; | ||
+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: | ||
+ | solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: | ||
+ | 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; | ||
+ | height:62.25pt'> | ||
+ | <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0034__x0020_Imagen" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute; | ||
+ | left:0;text-align:left;margin-left:22.6pt;margin-top:5.5pt;width:54.75pt; | ||
+ | height:52.5pt;z-index:251657728;visibility:visible;mso-wrap-style:square; | ||
+ | mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; | ||
+ | mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; | ||
+ | mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; | ||
+ | mso-position-horizontal-relative:text;mso-position-vertical:absolute; | ||
+ | mso-position-vertical-relative:text;mso-width-percent:0; | ||
+ | mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> | ||
+ | <v:imagedata src="TablasAromaYResistencia_archivos/image006.png" o:title=""/> | ||
+ | <w:wrap type="square"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=73 height=70 | ||
+ | src="TablasAromaYResistencia_archivos/image006.png" align=left hspace=12 | ||
+ | v:shapes="_x0034__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; | ||
+ | color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: | ||
+ | EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i><i><span | ||
+ | lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: | ||
+ | "Times New Roman";mso-bidi-font-family:"Times New Roman";color:black; | ||
+ | mso-fareast-language:ES-MX'> | ||
+ | <o:p></o:p> | ||
+ | </span></i></p> | ||
+ | </td> | ||
+ | <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; | ||
+ | border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: | ||
+ | accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; | ||
+ | mso-border-right-themecolor:accent6;mso-border-right-themetint:153; | ||
+ | mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; | ||
+ | mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; | ||
+ | mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: | ||
+ | solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal'><span lang=ES-MX style='font-size:12.0pt; | ||
+ | font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: | ||
+ | "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p></span></p> | ||
+ | <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; | ||
+ | text-align:center;line-height:normal'> </p></td> | ||
+ | <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: | ||
+ | none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; | ||
+ | mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: | ||
+ | accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; | ||
+ | mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: | ||
+ | solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: | ||
+ | 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; | ||
+ | mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> | ||
+ | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: | ||
+ | justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; | ||
+ | mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; | ||
+ | color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of | ||
+ | 6pb responsible for transcription stop (Huang, 206).<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div></center> | ||
<p><b>References</b></p> | <p><b>References</b></p> |
Revision as of 18:55, 13 June 2014
Resistance Module
Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need E. Coli to survive a high salinity environment to allow it to capture Na ions and remove the salt concentration of the water.
In order to accomplish our project goal, we have to change E.coli metabolism and make it stronger, more resistant and more efficient than in normal E.coli bacteria.
Last discoveries show IrrE as a protein capable of change the E.coli metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).
¿What does IrrE do?
The protein IrrE originates from Deinococcusradiodurans, and initially, this gene provides resistance to radiation. But when transformed in E. Coli, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in E. Coli with this biobrick.
Image 1. This graph done by UCL iGEM team 2012, shows how IrrE biobrick increased the salt concentration resistance in E. Coli compared with the results from Tu Delf iGEM team 2010.
How it works?
IrrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, IrrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, E. Coli becomes more salt tolerant (UCL, 2012).
Image 2. This diagram shows the effect of IrrE protein on E. Coli metabolism.
Other teams that use it:
London 2012: They propose to confer salt tolerance on E. Coli by linking the salt tolerance gene encoding the protein IrrE (BBa_K729001) to a constitutive promoter (BBa_J23119).
Image 3. Part designed by UCL iGEM team 2012 for the irrE protein.
IrrEparts description :
IMAGE |
CODE |
DESCRIPTION |
|
|
In
the specific case of our bacteria, it helps to continuously transcribing the IrrE gene in order to make bacteria resist high
concentration of salt. This promoter has a length
of 35 pb (Anderson, 2006). |
|
|
This
specific is RBS based on Elowitz
repressilator. It is very common to see in many iGEM projects. It
has a length of 12 pb. (Mahajan, Marinescu, Chow, Wissner-Gross,
& Carr, 2003). |
|
Gene
that produces IrrE, a substance that changes the
bacteria’s metabolism and allows bacteria to survive to extreme conditions,
some examples could be high UV rays exposition, or high salt concentration
levels in an aquatic environment, oxidative or thermal shock. It has a length of 933pb (Sohrabi, 2012). |
|
|
|
Part made of
6pb responsible for transcription stop (Huang, 206). |
References
Huang, H. (2006, August 30). Part:BBa_B1002. . Retrieved August 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002
Part:BBa_B0034. (2013, January 31). . Retrieved , from http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034
Part:BBa_J23119. (2006, August 24). . Retrieved April 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119
Sohrabi, B. (2012, June 27). Part:BBa_K729001. . Retrieved , from http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001
UCLiGEM Team. (2012). irre module. Retrieved the 31, March of 2014 from https://2012.igem.org/Team:University_College_London/Module_5