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Team:Elan Vital South Korea/n overview
From 2014hs.igem.org
Jan.9 - Jan 11
Ye Eun Lee met with a participant of IGEM 2012 and was informed IGEM. She contacted high school students who were interested in biology to form a team.
Jan.12 - Jan 18
Elan Vital has formed for 2014 IGEM HS competition and started to search a lab and an instructor who will give them directions and guidelines.
Jan.26 - Feb.1
Elan Vital found professor Hwang’s lab in Gacheon university. Elan vital team is organized to participate in the iGem jamboree, and created the Facebook group.
Feb.16 - Feb. 22
Elan vital team had a kick-off meeting at Gacheon University, where the project, experiments and study topics were decided upon, and the general safety guidelines were given. Elan Vital decided to investigate MRSA.
Feb.23 - March 1
The team searched for essays on MRSA, and learned about MRSA by reading and discussing an article: “MRSA – what is it, and how do we deal with the problem?”
March 2 - March 8
The team made some LB plate with antibiotics included to test for resistance in bacterium. We used stock solutions with concentrations of 100mg/μl of ampicillin, 10mg/μl of gentamycin, 50mg/μl of kanamycin, and 5mg/μl of tetracycline. We then made LB plates with 1/1000 concentration of stock solutions. The team made the Elan Vital logo, and designed and made the team T-shirt.
March 9 - March 15
The team studied how the antibiotics concerned (ampicillin, gentamycin, kanamycin, and tetracycline) works. The team tested the MRSA for antibiotic resistance in the LB plates with antibiotics by placing the MRSA on the plate with the antibiotic, then observing its growth.
March 23 - March 29
The team extracted MRSA DNA via miniprep and transformed the competent E. coli with it. We then tested the transformed E. coli for antibiotic resistance by placing the E. coli on a LB plate with antibiotics, then observing tis growth. We also tested the antibiotic resistance of the competent E. coli by the same method. To learn more about the drug resistance of MRSA, we researched by reading the paper “Antimicrobial resistance: the example of Staphylococcus aureus.” The team informed the Bugil international class about MRSA and iGEM.
March30 - April 5
The team researched the dangers of MRSA, and then did a brief presentation on the dangers of MRSA in a nursing home, where MRSA could pose a dangerous threat. The team talked about future plans, and had a party at a local restaurant.
April 6 - April 12
The instructors only let us handle safe experiments only involving the plasmid DNA of MRSA (instead of the actual MRSA), but we wanted to have a first-hand experience of the experimentation, so we investigated the safety regulations of iGEM, WHO, and the Gacheon university safety committee.
April 13 - April 19
The team held a presentation about iGEM, the Elan Vital team, and the dangers of MRSA in seoincheon high school.
April 27 - May 3
The team grew MRSA first hand and tested it for resistance against Ampicillin Gentamycin, Tetracycline, and kanamycin.
May 4 - May 10
The team preformed miniprep on the bacteria that survived the antibiotics, and transformed the competent E. coli with the MRSA DNA. We then tested the transformed E. coli for antibiotic resistance. We also tested the antibiotic resistance of the competent E. coli as control. The team learned about the primers that are used in PCR.
May 18 - May 24
The team preformed miniprep on the transformed E. coli that survived, and preformed PCR using the DNA from the MRSA that survived initially, and the DNA from the transformed E. coli that survived. We then preformed gel electrophoresis with the DNA that amplified sufficiently.
June 1 - June 7
The team held a conference at a restaurant in Cheonan, Cheongmaegol, where the results of the experiment are and ideas for the team video are discussed. The team held public awareness event at Goyang Bus Terminal, where we explained the danger of MRSA and introduced our team: Elan vital.
June 8 - June 14
The team have made a short video introducing the Elan vital team.
